Enzymatic production and physicochemical and functional properties of sorghum protein isolates.

Grain sorghum has emerged as a promising source for producing alternative proteins, yet current extraction methods lack efficiency. In this study, a novel enzymatic approach using α-amylase and cellulase on sorghum materials was developed to address this challenge. Comparisons were made among the proteins isolated from dry-milled sorghum flours, wet-milled sorghum gluten meals, and sorghum dried distiller's grains (DDG).

Optimizing the degradation of aflatoxin B in corn by Trametes versicolor and improving the nutritional composition of corn.

Background: Corn, being an important grain, is prone to contamination by aflatoxin B (AFB ), and AFB -contaminated corn severely endangers the health of humans and livestock. Trametes versicolor, a fungus that can grow in corn, possesses the ability to directly degrade AFB through its laccase. This study aimed to optimize the fermentation conditions for T.

Edible fungi efficiently degrade aflatoxin B in cereals and improve their nutritional composition by solid-state fermentation.

Aflatoxin B (AFB) is extremely harmful to human and livestock. Laccase, a green catalyst, has been shown to effectively degrade AFB and can be obtained from edible fungi. The objective of this study was to screen edible fungi with high laccase activity and determine their effects on the degradation of AFB in cereals and the nutritional composition of the cereals through solid-state fermentation.

Cloning and expression of the catalase gene (KatA) from Pseudomonas aeruginosa and the degradation of AFB by recombinant catalase.

Background: Aflatoxin B (AFB ) poses a severe threat to human and animal health. Countries worldwide have invested considerable manpower and material resources in degrading aflatoxins. Enzyme degradation is the most efficient and environmentally friendly approach for modifying aflatoxin into less toxic molecules.

Transcriptome Analysis of Reveals Genes Associated With Carotenoid Synthesis and Identification of the Function of the Gene.

, a valuable edible and medicinal fungus, has attracted increasing attention because of its various bioactive ingredients. However, the biosynthetic pathway of carotenoids is still unknown due to lack of transcriptome information. To uncover genes related to the biosynthesis of carotenoids, the transcriptomes of mycelia CM10_D cultured under dark conditions and mycelia CM10_L cultured under light exposure conditions were sequenced.

Purification and structural characterization of a novel natural pigment: cordycepene from edible and medicinal mushroom Cordyceps militaris.

In the present work, a novel cordycepic pigment was successfully isolated and identified from Cordyceps militaris, as well as named as cordycepene (CHNO), according to the long unsaturated conjugated polyene structural characteristic. Cordycepene is sensitive to light, high temperature (≥ 60 °C), and acidic condition (pH ≤ 3), but possesses high stability against metal ions, and under alkaline and neutral conditions. Cordycepene shows a comparable DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging activity at higher concentration (≥ 2 mg/mL) to vitamin C.

Advances in research on Cordyceps militaris degeneration.

As a highly valued fungus, Cordyceps militaris has been widely used all over the world. Although the wild resources of C. militaris are limited, the fruiting bodies of C.

Optimization of Cultivation Conditions of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes) for the Highest Antioxidant Activity and Antioxidant Content.

Ganoderma lucidum is a famous medicinal mushroom that is rich in antioxidants. The content of antioxidant components of grains can be effectively improved by G. lucidum as the fermenting strain.

Targeted Gene Deletion in Cordyceps militaris Using the Split-Marker Approach.

The macrofungus Cordyceps militaris contains many kinds of bioactive ingredients that are regulated by functional genes, but the functions of many genes in C. militaris are still unknown. In this study, to improve the frequency of homologous integration, a genetic transformation system based on a split-marker approach was developed for the first time in C.