Glabridin exhibits potent inhibitory effects against Toxoplasma gondii in vitro and in vivo.

Background: Toxoplasma gondii is an obligate protozoan parasite capable of infecting a wide range of warm-blooded animals and humans. Current treatment options, primarily pyrimethamine and sulfadiazine, have limitations, such as high recurrence rates, long treatment durations, and limited effectiveness against T. gondii.

Provenance and sedimentation of Pu and Np in the northern Taiwan Strait suffering intensive land-ocean interaction.

The China Sea is faced with a heightened risk of anthropogenic radionuclide contamination, whose provenance, scavenging and migration are imperative to investigate to provide the background and nuclear safety emergency assessment. This study pioneers the measurement of anthropogenic plutonium and neptunium (Pu and Np) concentrations and atom ratios (Pu/Pu and Np/Pu) in sediment cores from the northern Taiwan Strait and the adjacent East China Sea using SF-ICP-MS, exploring their applications and characteristics. Typical vertical profiles confirm that Pu and Np serve as geochronological tools, with the Pu/Pu atom ratio as a fingerprint refining the chronology.

Immunization of BALB/c Mice with Killed Tachyzoites of against Acute Toxoplasmosis.

Background: with widespread distribution infects over one third of human populations in the world and can cause serious life-threatening diseases especially for the immunodeficient patients in acute toxoplasmosis. As the clinical pharmaceutical drugs with severe side effects for treatment and non-ideal extant vaccines for prevention, more work starves to be done for keeping advantages in the athletics.

Methods: Aluminum adjuvant and hybrid formaldehyde-killed tachyzoites of RH and GT1 isolates were prepared to intramuscularly immunize BALB/c mice for five times at 0, 3, 7, 14 and 21 days post first injection.

In Vitro Virulence Contrast of Seven Genetically Distinct Toxoplasma gondii Isolates After Rejuvenation In Vivo.

Background: In the past for more than 100 years at least 300 genotypes of Toxoplasma gondii were recorded and several traditional isolates such as RH, GT1, ME49, PRU and VEG were used repeatedly to clarify the pathogenic mechanisms and the epidemiological significance to human, but for if their virulence was mutative post-iterative passages it remains confused.

Objective: Therefore, in the study, seven genetically distinct T. gondii including C7 and PYS previously discovered in China were reidentified by sequencing the head of hsp40 locus to distinguish their virulence in vitro post-rejuvenation in vivo.

Ultrasound features affecting the sample adequacy after fine-needle aspiration of thyroid nodules with different risk stratification.

Background: The inadequacy samples caused by the internal characteristic structure of thyroid nodules are difficult to be solved.

Objective: To evaluate the ultrasound features affecting the sample adequacy after fine-needle aspiration (FNA) of thyroid nodules with different risk stratification.

Methods: 592 thyroid nodules that underwent ultrasound-guided FNA were included in this retrospective study.

Distinctive distribution and migration of global fallout plutonium isotopes in an alpine lake and its implications for sediment dating.

This work investigated plutonium (Pu) isotopes in sediment cores collected from an alpine lake (Lake Heinongpo with 3779 m above sea level) in Southwestern China. Pu/Pu atom ratios in all sediment samples showed the typical global fallout values of ∼0.18 without any influences from other Pu contaminant sources.

Toxoplasma invasion delayed by TgERK7 eradication.

Toxoplasma gondii causes serious clinical toxoplasmosis in humans mostly due to its asexual life cycles, which can be artificially divided into five tightly coterminous stages. Any radical or delay for the stage will result in tremendous changes immediately behind. We previously demonstrated that TgERK7 is associated with the intracellular proliferation of T.

Immune Responses Induced by pVAX/ERK7 against Infection in BALB/c Mice.

Background: can infect all the warm-blooded vertebrates and cause serious toxoplasmosis. Extracellular signal-regulated kinase 7 in (ERK7) mediated the proliferation of this parasite may be a potential vaccine candidate. Thus, immune responses induced by ERK7 were investigated in this study using a DNA vaccine strategy.

Devitalization of the immune mapped protein 1 undermines the intracellular proliferation of Toxoplasma gondii.

The intracellular protozoan Toxoplasma gondii infects approximately one-third of the world's population as well as various animals, causing toxoplasmosis. However, there remains a need to define the functions of newly identified genes of T. gondii.

Immunization with plasmid DNA expressing Heat Shock Protein 40 confers prophylactic protection against chronic Toxoplasma gondii infection in Kunming mice.

Toxoplasma gondii causes one of the most common protozoal diseases of humans and animals worldwide. With the aim of designing an effective vaccine against T. gondii infection, we examined the immunogenicity of a DNA vaccine expressing heat shock protein 40 (HSP40) against challenge with T.

Sources of plutonium isotopes and Cs in coastal seawaters of Liaodong Bay and Bohai Strait, China and its environmental implications.

In order to investigate the sources of plutonium in seawaters of Liaodong Bay and Bohai Strait, China, surface seawater samples were collected and analyzed for Pu and Cs by radiochemical separation combined with ICP-MS and γ-spectrometry, respectively. A large variation of Pu activities was observed, ranging from 1.993 to 29.

[Analysis of the storage time in paraformaldehyde-fixed T cells and their subsets in Kunming mice].

Objective To determine the expiration dates of CD3eCD4 T and CD3eCD8a T lymphocytes of Kunming mice. Methods The sterile spleen and anticoagulant blood of Kunming mice were collected and used to prepare the single-cell suspensions. After CD3e, CD4 and CD8a of T cells were stained with fluorophore-conjugated monoclonal antibodies, the samples were fixed with paraformaldehyde (PFA) and stored at 4DegreesCelsius in dark followed by detection using three-color flow cytometry at several specific time points.

Intervention of childhood and adolescents obesity in Shantou city.

Objectives: The purpose of this study was to evaluate the effects of multicomponent school based intervention constituted of diet modification, regular exercise and psychosocial consultation on body status in overweight and obese children and adolescents. And to come up with an appropriate intervention protocol for controlling children and adolescents obesity in Shantou city.

Methods: Two schools were randomised to intervention group and control group respectively.