The relationship between childhood psychological abuse and social media addiction among college students: the mediating role of fear of missing out and the moderating role of left-behind experience.

Introduction: Mobile social media is a new type of online media that is participatory, open, and communicative, among other characteristics. Due to the increasing popularity of social media, this technology has become an indispensable part of people's social lives. This study aims to examine the relationship between childhood psychological maltreatment and social media addiction among university students as well as the mediating role of fear of missing out and the moderating effect of left-behind experience in this context.

Establishment and application of Agrobacterium-delivered CRISPR/Cas9 system for wild tobacco () genome editing.

Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR-Cas9) system has been widely applied in cultivated crops, but limited in their wild relatives. is a typical wild species of genus that is globally distributed as a horticultural plant and well-studied as a self-incompatibility model. It also has valuable genes for disease resistance and ornamental traits.

Characterization of the CMS genetic regulation through comparative complete mitochondrial genome sequencing in Nicotiana tabacum.

Mitochondrial genomes (mitogenomes) of flowering plants vary greatly in structure and size, which can lead to frequent gene mutation, rearrangement, or recombination, then result in the cytoplasmic male sterile (CMS) mutants. In tobacco (Nicotiana tabacum), suaCMS lines are widely used in heterosis breeding; however, the related genetic regulations are not very clear. In this study, the cytological observation indicated that the pollen abortion of tobacco suaCMS(HD) occurred at the very early stage of the stamen primordia differentiation.

CRISPR/Cas9-mediated seamless gene replacement in protoplasts expands the resistance spectrum to TMV-U1 strain in regenerated Nicotiana tabacum.

CRISPR/Cas-based genome editing is now extensively used in plant breeding and continues to evolve. Most CRISPR/Cas current applications in plants focus on gene knock-outs; however, there is a pressing need for new methods to achieve more efficient delivery of CRISPR components and gene knock-ins to improve agronomic traits of crop cultivars. We report here a genome editing system that combines the advantages of protoplast technologies with recent CRISPR/Cas advances to achieve seamless large fragment insertions in the model Solanaceae plant Nicotiana tabacum.

Teacher Online Informal Learning as a Means to Innovative Teaching During Home Quarantine in the COVID-19 Pandemic.

The home quarantine in the COVID-19 pandemic has created challenges for teaching across the world and called for innovative teaching, as well as teachers' learning. Given the rapid development of teachers' online learning and teaching, identifying effective ways to facilitate innovative teaching under such challenging conditions is a critical issue. Although researchers have realized that workplace informal learning (IL) increasingly reveals its potential value to individual development, the relationship between IL and innovation has been under-explored.

A Motivational Mechanism Framework for Teachers' Online Informal Learning and Innovation During the COVID-19 Pandemic.

Online informal learning (IL) spreads quickly in the COVID-19 Pandemic. Studies have predicted that both online and workplace IL have potential value to individual and organization development, whereas the study on its link with innovation remains scarce. IL is an individualized learning pattern different from formal learning, and its functioning mechanism on innovation will deepen our understanding of the relationship between learning and innovation.

The complete chloroplast genome sequence of (Solanaceae).

In present study, we report the complete chloroplast genome of , a species endemic to eastern coast of Australia. The total genome size of is 156,073 bp in length, containing a large single-copy region of 86,672 bp, a small single-copy region of 18,581 bp, and two inverted repeat regions of 25,410 bp. The all GC content of chloroplast genome is 38.

Phosphate adsorption performance and mechanisms by nanoporous biochar-iron oxides from aqueous solutions.

To evaluate the adsorption mechanism and performance of phosphate onto the composite of low-cost biochar and iron oxide, four biochar-iron oxides, namely biochar-magnetite (BC-M), biochar-ferrihydrite (BC-F), biochar-goethite (BC-G), and biochar-hematite (BC-H), were prepared by fabricating iron oxide to porous biochar. The biochar-iron oxides had huge surface areas of 691-864 m/g and average pore diameters of 3.4-4.

Adsorption of heavy metals by l-cysteine intercalated layered double hydroxide: Kinetic, isothermal and mechanistic studies.

To understand the interaction mechanisms between heavy metals and functional groups modified nanomaterials, the l-cysteine (Cys) intercalated MgAl-layered double hydroxide (MgAl-Cys-LDH) was designed by a facile co-precipitation method and used to remove Cu(II), Pb(II) and Cd(II) in water solutions. The XRD, FTIR, SEM, TEM, EDS and XPS characterization analyses proved the carboxyl, thio and amido groups were successfully introduced into MgAl-LDH. The possible mechanisms were analyzed by the XPS and XRD spectra and involved the precipitation of metal hydroxides or sulfides, surface complexation with abundant surface groups, and the isomorphic substitution of Mg(II).

Identification of two additional plasmodesmata localization domains in the tobacco mosaic virus cell-to-cell-movement protein.

Despite decades of intensive studies, the failure to identify plasmodesmata (PD) localization sequences has constrained our understanding of Tobacco mosaic virus (TMV) movement. Recently, we identified the first PD localization signal (major PLS) in the TMV movement protein (MP), which encompasses the first 50 amino acid residues of the MP. Although the major PLS is sufficient for PD targeting, the efficiency is lower than the full-length TMV MP.

Adsorption of phosphate from aqueous solution by vegetable biochar/layered double oxides: Fast removal and mechanistic studies.

Two biochars, from Chinese cabbage (Cc, Brassica rapa pekinensis) and rape (Ra, Brassia campestris L.), were used to prepare biochar/Mg-Al layered double oxides (LDOs) as adsorbents for phosphate removal from aqueous solution. The biochar/LDOs were horizontally alternated lamellar particles and had abundant groups of oxides and biochars.

Efficient and fast removal of Pb and Cd from an aqueous solution using a chitosan/Mg-Al-layered double hydroxide nanocomposite.

A new nanocomposite based on chitosan (CS) and Mg-Al-layered double hydroxide (Mg-Al-LDH) was prepared using an emulsion-crosslinking method. CS was immobilized within the Mg-Al-LDH matrix to form the CS-LDH after crosslinking by epichlorohydrin. The characterization of XPS, SEM, TEM, FTIR, and BET analysis showed that the CS-LDH had a high specific surface area and contained many different functional groups.

Non-Structural Protein NSm of Is an Avirulence Factor Recognized by Resistance Genes of Tobacco and Tomato via Different Elicitor Active Sites.

(TSWV) is one of the most destructive viral pathogens of plants. Recently, a single dominant gene conferring complete resistance to TSWV (RTSW) was identified in and introgressed into cultivated tobacco (). However, whether the TSWV carries an avirulence (Avr) factor directed against RTSW remains obscure.

Removal of Cu, Cd and Pb from aqueous solutions by magnetic alginate microsphere based on FeO/MgAl-layered double hydroxide.

In this work, the magnetic alginate microsphere of FeO/MgAl-LDH (FeO/LDH-AM) was prepared by immobilizing the FeO/LDH with calcium alginate (CA) and was used to remove Cd, Pb, and Cu from aqueous solutions. The obtained FeO/LDH-AM was characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, energy dispersive spectroscopy, X-ray photoelectron spectroscopy, and Brunauer-Emmett-Teller surface area determination. The results indicated that the surface groups of the alginate and LDH were retained and so was the crystal structure in the alginate microsphere.

Transcriptomic profile of tobacco in response to Tomato zonate spot orthotospovirus infection.

Background: Tomato zonate spot virus (TZSV), a dominant species of thrips-transmitted orthotospoviruses in Yunnan and Guangxi provinces in China, causes significant loss of yield in lots of crops and is a major threat to incomes of rural families. However, the detailed molecular mechanism of crop disease caused by TZSV remains obscure.

Methods: Next-generation sequencing (NGS)-based transcriptome analysis (RNA-seq) was performed to investigate and compare the gene expression changes in systemic leaves of tobacco upon infection with TZSV and mock-inoculated plants as a control.

Genomes and virulence difference between two physiological races of Phytophthora nicotianae.

Background: Black shank is a severe plant disease caused by the soil-borne pathogen Phytophthora nicotianae. Two physiological races of P. nicotianae, races 0 and 1, are predominantly observed in cultivated tobacco fields around the world.

Kinetic, isotherm and thermodynamic investigations of phosphate adsorption onto core-shell Fe₃O₄@LDHs composites with easy magnetic separation assistance.

In this study, three different magnetic core-shell Fe3O4@LDHs composites, Fe3O4@Zn-Al-, Fe3O4@Mg-Al-, and Fe3O4@Ni-Al-LDH were prepared via a rapid coprecipitation method for phosphate adsorptive removal. The composites were characterized by XRD, FTIR, TEM, VSM and BET analyses. Characterization results proved the successful synthesis of core-shell Fe3O4@LDHs composites with good superparamagnetisms.

Diethanolamine-modified magnetic fluorescent Fe3O4@ZnS nanoparticles for ultrasensitive detection and removal of Cu2+.

Currently, growing attention has been paid to the sensitive determination and removal of Cu2+ because excessive levels of Cu2+ could do harm to organisms. Herein, a novel diethanolamine-modified magnetic fluorescent Fe3O4@ZnS nanoparticle (MFNP) for simultaneous detection and removal of Cu2+ was designed and synthesized through dithiocarbamate linkage strategy. The characterization of MFNP was confirmed by transmission electron microscope (TEM), infrared (IR) and emission spectra.

Development of a novel water-soluble magnetic fluorescent nanoparticle for the selective detection and removal of Cu2+.

Recently, much attention has been paid to the selective detection and removal of Cu2+ because an excess of Cu2+ can harm the environment and living systems. Herein, we developed a novel water-soluble di-2-picolylamine/proline co-modified Fe3O4@ZnS magnetic fluorescent nanoparticle (MFNP-Cu) for the selective detection and removal of Cu2+ through a dithiocarbamate linkage strategy. The characterization of MFNP-Cu was confirmed by x-ray diffraction (XRD), transmission electron microscope (TEM), magnetization hysteresis loops, infrared (IR) and emission spectra.

Ultrasensitive electrochemical immunosensor for zeranol detection based on signal amplification strategy of nanoporous gold films and nano-montmorillonite as labels.

Nano-montmorillonites belong to aluminosilicate clay minerals with innocuity, high specific surface area, ion exchange, and favorable adsorption property. Due to the excellent properties, montmorillonites can be used as labels for the electrochemical immunosensors. In this study, nano-montmorillonites were converted to sodium montmorillonites (Na-Mont) and further utilized for the immobilization of thionine (TH), horseradish peroxidase (HRP) and the secondary anti-zeranol antibody (Ab(2)).

Nanoporous PtCo-based ultrasensitive enzyme-free immunosensor for zeranol detection.

Nanoporous PtCo alloy was designed as an antibody carrier for preparation of a highly sensitive immunosensor. The immunosensor was constructed by assembling the capture zeranol antibody on thionine decorated graphene nanosheets modified glassy carbon electrode. With an enzyme-free immunosensor mode, the nanoporous PtCo alloy, synthesized by dealloying method, had shown strong electrocatalytic activity toward antigen-antibody reaction.

Label-free electrochemical immunosensor for prostate-specific antigen based on silver hybridized mesoporous silica nanoparticles.

Due to high sensitivity and good selectivity, electrochemical immunosensor is often used to detect tumor markers. Prostate-specific antigen (PSA) is the most validated tumor marker for prostate cancer. In this work, mesoporous silica nanoparticles (MSNs) were used to increase the fixation capacity of primary antibody, and silver nanoparticles (Ag NPs) were used to enhance the electron transfer rates.

A reusable naphthalimide-functionalized magnetic fluorescent nanosensor for the simultaneous determination and removal of trace Hg2+ in aqueous solution.

On the basis of high selective and sensitive interaction of 1,8-naphthalimide with Hg(2+) and the formation of stable neutral imide-Hg-imide complexes, we designed and synthesized a novel magnetic fluorescent sensor (S1) employed Fe(3)O(4) magnetic nanoparticles and 1,8-naphthalimide fluorescent sensor. Under optimum conditions, S1 exhibits the high selectivity toward Hg(2+) over other metal ions, with the detection limit of 1.03×10(-8)M.

Label-free electrochemical immunosensor based on graphene/methylene blue nanocomposite.

For the specificity of prostate cancer markers, prostate specific antigen (PSA) has been widely used in prostate cancer screening, diagnosis, and treatment after monitoring. In normal male serum, PSA can only be detected in traces of 0-4 ng mL(-1). In this paper, we constructed an electrochemical immunosensor for PSA detection using a nanocomposite film of graphene sheets-methylene blue-chitosan (GS-MB-CS) as electrode material.