UHPLC-MS/MS-based untargeted lipidomics analysis of septic patients.

Background: Lipids take part in many pathophysiological processes of sepsis, thus, the variation of lipid composition may have clue on the severity and pathogen to sepsis. The objective of our study is to expand the profile of lipid compositions and screen potential biomarkers in intensive care unit (ICU) patients with sepsis.

Methods: Patients admitted to the ICU clearly diagnosed with celiac sepsis were included in this prospective study.

Machine learning for infection risk prediction in postoperative patients with non-mechanical ventilation and intravenous neurotargeted drugs.

Drug efficacy can be improved by understanding the effects of anesthesia on the neurovascular system. In this study, we used machine learning algorithms to predict the risk of infection in postoperative intensive care unit (ICU) patients who are on non-mechanical ventilation and are receiving hydromorphone analgesia. In this retrospective study, 130 patients were divided into high and low dose groups of hydromorphone analgesic pump patients admitted after surgery.

[Nontargeted lipidomic analysis of sera from sepsis patients based on ultra-high performance liquid chromatography-mass spectrometry/mass spectrometry].

Objective: To analyze the changes of serum lipidomics in patients with sepsis and healthy controls, search for the differences of lipid metabolites, and reveal the changes of lipidomics in the process of sepsis.

Methods: A prospective observational study was conducted. From September 2019 to April 2020, morning blood samples of upper extremity superficial veins were collected from 30 patients with definite sepsis diagnosed in intensive care unit (ICU) of Shanxi Bethune Hospital and 30 age-matched healthy subjects during the same period.

Metal-doped carbon dots as peroxidase mimic for hydrogen peroxide and glucose detection.

Carbon dots (CDs) have several superior characteristics including sufficient carbon sources, easy preparation, no toxicity, and high catalytic efficiency as a new kind of nanozyme. Herein, Ce-doped carbon dots (Ce-CDs), Cr-doped carbon dots (Cr-CDs), Cu-doped carbon dots (Cu-CDs), Fe-doped carbon dots (Fe-CDs), Mn-doped carbon dots (Mn-CDs), and non-metal-doped carbon dots (0-CDs) were synthesized to explore the detection of hydrogen peroxide (HO) and glucose as peroxidase mimic. The prepared CDs could efficiently oxidize the colorless 3,3',5,5'-tetramethylbenzidine (TMB) into blue oxTMB in the presence of HO.

Effects of blue-green LED lights with two perceived illuminance (human and poultry) on immune performance and skeletal development of layer chickens.

Light is one of the essential environmental factors in the production process of laying hens, which can directly affect their behavior, growth and development, and production performance. The spectral sensitivity of humans is different from that of poultry, and the perceived illuminance units of human and poultry are lux and clux, respectively. If the light management of laying hen production is carried out according to human perceived illuminance, the growth and development of laying hens during pullet rearing may be adversely affected due to the discomfort of the perceived illuminance.

Rehabilitation of Sepsis Patients with Acute Kidney Injury Based on Intelligent Medical Big Data.

The objective of this study was to explore rehabilitation of patients with acute kidney injury (AKI) treated with Xuebijing injection by using intelligent medical big data analysis system. Based on Hadoop distributed processing technology, this study designed a medical big data analysis system and tested its performance. Then, this analysis system was used to systematically analyze rehabilitation of sepsis patients with AKI treated with Xuebijing injection.

Nodular malignant melanoma in vulvar skin without pigmentation: a case report.

Background: Polypoid nodule growing without apparent pigmentation on the vulvar skin usually reminds us of the diagnostic pitfall, which is commonly and mistakenly diagnosed as other types of tumors. Although there are several manifestations of amelanotic melanoma are known, these malignancies are usually pigmented because they are derived from melanocytes containing melanin. However, amelanotic melanomas are easily misdiagnosed or their diagnoses were commonly delayed due to lack of pigmentation.

Imaging presentation and postoperative recurrence of peliosis hepatis: A case report.

Background: Peliosis hepatis (PH) is a rare benign lesion of vascular origin with a pathological characteristic of multiple blood-filled cavities in the liver parenchyma. It is commonly misdiagnosed due to its lack of specificity in clinical presentation and laboratory test results. Herein, a case of a patient with PH who was misdiagnosed with hepatic echinococcosis before operation to remove the lesions was analyzed, with an emphasis on the computed tomography and magnetic resonance imaging characteristics of PH.

A stable and sensitive enzyme-linked immunosorbent assay (ELISA) for the determination of metsulfuron-methyl residues in foods.

A hapten of metsulfuron-methyl was successfully designed and synthesized from 2-methylester-phenylsulfonamide and succinic anhydride, and the polyclonal antibody against metsulfuron-methyl was prepared by immunization procedure with the hapten-bovine serum albumin conjugate. A stable and sensitive direct competitive enzyme-linked immunosorbent assay (dcELISA) method had been developed under the optimal conditions. The sensitivity (IC ) was 37.

Predicting the Risk of Acute Kidney Injury in Patients After Percutaneous Coronary Intervention (PCI) or Cardiopulmonary Bypass (CPB) Surgery: Development and Assessment of a Nomogram Prediction Model.

BACKGROUND We sought to create a model that incorporated ultrasound examinations to predict the risk of acute kidney injury (AKI) after percutaneous coronary intervention (PCI) or cardiopulmonary bypass (CPB) surgery. MATERIAL AND METHODS A total of 292 patients with AKI after PCI or CPB surgery were enrolled for the study. Afterwards, treatment-related information, including data pertaining to ultrasound examination, was collected.

The Infective Endocarditis with Recurrent Epistaxis in a Young Patient: A Case Report.

Epistaxis is a common emergency, and its main causes are hypertensive crisis and trauma. Nasal packing is the primary treatment. After active symptomatic treatment, the symptoms of epistaxis effectively can be controlled.

Efficient Multiple Sequences Alignment Algorithm Generation Components Assembly Under PAR Framework.

As a key algorithm in bioinformatics, sequence alignment algorithm is widely used in sequence similarity analysis and genome sequence database search. Existing research focuses mainly on the specific steps of the algorithm or is for specific problems, lack of high-level abstract domain algorithm framework. Multiple sequence alignment algorithms are more complex, redundant, and difficult to understand, and it is not easy for users to select the appropriate algorithm; some computing errors may occur.

Research on Components Assembly Platform of Biological Sequences Alignment Algorithm.

After years of development, the complexity of the biological sequence alignment algorithm is gradually increasing, and the lack of high abstract level domain research leads to the complexity of its algorithm development and improvement. By applying the idea of software components to the design and development of algorithms, the development efficiency and reliability of biological sequence alignment algorithms can be effectively improved. The component assembly platform applies related assembly technology, which simplifies the operation difficulty of component assembly and facilitates the maintenance and optimization of the algorithm.

Effects of a two-phase mixed color lighting program using light-emitting diode lights on layer chickens during brooding and rearing periods.

The behavior, growth and development, and production performance of poultry are affected by the light environment. The influence of light results from a combination of light sources, light intensity, light color, and the photoperiod regimen. With light-emitting diode (LED) lamps applied in poultry housing systems, specific light colors are desired for each time period for layer chickens.

Synthesis of anionic ionic liquids@TpBd-(SO) for the selective adsorption of cationic dyes with superior capacity.

The discharge of industrial printing and dyeing wastewater is one of the main reasons for the increasing water shortage and deterioration. The treatment of dyestuff wastewater is an issue and needs to be urgently solved. In this work, anionic ionic liquid functional covalent organic materials (COMs) were firstly synthesized and used for the selective adsorption of cationic dyes.

Effects of B-Wave Ultraviolet Supplementation Using Light-Emitting Diodes on Caged Laying Hens during the Later Phase of the Laying Cycle.

Caged laying hens are prone to calcium deficiencies, resulting in osteoporosis and egg quality deterioration during the later phase of the laying cycle. Fluorescent light and light-emitting diodes (LEDs), which are widely used in poultry houses now, are both deficient in ultraviolet (UV) light, the lack of which is detrimental to chickens' welfare and health. This study was conducted to investigate the effects of UVB light supplementation using LEDs on the bone traits, blood parameters, laying performance, and egg quality for caged laying hens at 68-75 weeks.

Effects of LED Light Color and Intensity on Feather Pecking and Fear Responses of Layer Breeders in Natural Mating Colony Cages.

Natural mating colony cages for layer breeders have become commonplace for layer breeders in China. However, feather pecking (FP) and cannibalism are prominent in this system. The objective of this study was to investigate the effects of four light-emitting diode (LED) light colors (white: WL, red: RL, yellow-orange: YO, blue-green: BG) with two light intensities for each color, on FP, plumage condition, cannibalism, fear, and stress.

Effects of nest boxes in natural mating colony cages on fear, stress, and feather damage for layer breeders1,2,3.

Colony cages are commonly used in China for the natural mating of layer breeders. However, feather pecking (FP) is a major problem in this system, and feather damage mainly due to FP needs to be alleviated. The objective of this study was to investigate the effects of nest boxes provided in colony cages.

Effects of different claw-shortening devices on claw condition, fear, stress, and feather coverage of layer breeders.

Natural mating colony cage systems for parent-stock layer breeders have been widely adopted by many commercial farms in China. However, the environment is still relatively barren, so enrichment devices such as claw abrasives can be provided. Hens living in wire cages lack the opportunity to abrade their claws, resulting in uncontrolled claw growth.

Electrochemiluminescence sensor based on upconversion nanoparticles and oligoaniline-crosslinked gold nanoparticles imprinting recognition sites for the determination of dopamine.

For the determination of dopamine (DA) in serum samples, a quenching-type electrochemiluminescence sensor (MIECLS) was constructed in this study. Upconversion nanoparticles (UCNPs) enhanced by covalent organic frameworks (COFs)-based hybrid and oligoaniline-crosslinked gold nanoparticles (AuNPs) imprinting recognition sites were introduced in electrochemiluminescence (ECL) system at the first time. The porous COFs-based hybrid with large specific surface area was modified on the electrode firstly to hold more UCNPs and imprinting recognition sites.

Effects of shRNA targeting maspin on invasion of gastric carcinoma SGC7901 cell line.

Maspin is a member of the serpin (serine protease inhibitor) family of protease inhibitors known to have tumor suppressor activity in diverse human cancers. However, maspin gene function and the molecular aspects in gastric carcinoma remain largely unclear. To investigate the effects of maspin on invasion of gastric carcinoma SGC7901 cell line and the underlying molecular mechanism involved in this process, we cloned short hairpin oligoes (shRNA) targeting maspin into plasmid pGenesil-1.

Prognostic indicators of patients with acute kidney injury in intensive care unit.

Background: Acute kidney injury (AKI) is associated with a high mortality. This study was undertaken to detect the factors associated with the prognosis of AKI.

Methods: We retrospectively reviewed 98 patients with AKI treated from March 2008 to August 2009 at this hospital.