A high-throughput multiplex genetic detection system for Helicobacter pylori identification, virulence and resistance analysis.

Aim: We established a high-throughput multiplex genetic detection system (HMGS) for identification of Helicobacter pylori with concomitant analysis of virulence and drug resistance.

Materials & Methods: Confirmed 132 H. pylori cultures from gastric biopsies were screened by 20-gene site-HMGS, sequencing and E-test.