Random Sanitization in DNA Information Storage Using CRISPR-Cas12a.

DNA information storage provides an excellent solution for metadata storage due to its high density, programmability, and long-term stability. However, current research primarily focuses on the processes of storing and reading data, lacking comprehensive solutions for secure metadata wiping. Herein, we present a method of random sanitization in DNA information storage using CRISPR-Cas12a (RSDISC) based on precise control of the thermodynamic energy of primer-template hybridization.

DNA-framework-based multidimensional molecular classifiers for cancer diagnosis.

A molecular classification of diseases that accurately reflects clinical behaviour lays the foundation of precision medicine. The development of in silico classifiers coupled with molecular implementation based on DNA reactions marks a key advance in more powerful molecular classification, but it nevertheless remains a challenge to process multiple molecular datatypes. Here we introduce a DNA-encoded molecular classifier that can physically implement the computational classification of multidimensional molecular clinical data.

Programming Super DNA-Enzyme Molecules for On-Demand Enzyme Activity Modulation.

Dynamic interactions of enzymes, including programmable configuration and cycling of enzymes, play important roles in the regulation of cellular metabolism. Here, we constructed a super DNA-enzymes molecule (SDEM) that comprises at least two cascade enzymes and multiple linked DNA strands to control and detect metabolism. We found that the programmable SDEM, which comprises glucose oxidase (GOx) and horseradish peroxidase (HRP), has a 20-fold lower detection limit and a 1.

The enhancement of enzyme cascading tetrahedral DNA framework modification.

Enzyme clustering is widely used in many organisms to increase the catalytic efficiency of cascade reactions. Inspired by nature, organizing enzymes within a cascade reaction also draws much attention in both basic research and industrial processes. An important step for organizing enzymes precisely is enzyme modification.

DNA Framework-Programmed Micronano Hierarchy Sensor Interface for Metabolite Analysis in Whole Blood.

A microsubstrate is of great value for constructing a microscale interface with highly improved sensing capabilities. What has been lacking, however, is the precisely nanoscale regulation of the probes that anchored on the interface of the microsubstrate. Here we employed tetrahedron DNA framework (TDF) to program the microscale biosensing interface for metabolite analysis.

Stepping gating of ion channels on nanoelectrode via DNA hybridization for label-free DNA detection.

Natural ion channels on cell membrane can gate the transport of ions and molecules by the conformational alteration of transmembrane proteins to regulate the normal physiological activities of cells. Inspired by the similarity of the conformation change under specific stimuli, here we introduce an ion channel gating model on a single nanoelectrode by anchoring DNA-gated switches on the very nanotip of gold nanoelectrode to mimic the response-to-stimulus behaviors of ion channels on bio-membranes. The surface-tethered DNA ion channels can be switched on by the Watson-Crick base pairing, which can alter the conformation of the tethered DNA from lying state to upright state.