Construction and regulation of aqueous-based Cerberus droplets by vortex mixing.

Hypothesis: The emerging aqueous-based Cerberus emulsion droplets with multi-domains behave as an excellent platform to design cyto-mimetic compartmentalization for fabrication of anisotropic biomimetic materials and microreactors. However, the ultralow water/water interfacial tension impedes fabrication of aqueous Cerberus droplets in batch-scale and precisely topology regulation especially under lack of deep understanding of w/w interface properties.

Experiments: Aqueous-based ternary phase diagram composed by salt, hydrophilic polymer and fluorocarbon compound is determined.

Destabilization mechanism of (W+W)/O reverse Janus emulsions.

Hypothesis: Reverse Janus emulsion, with droplets composed by "two rooms" of water phases, is a novel multiple emulsion attributed to excellent integration capability and biocompatibility. However, significant instability compared with normal Janus emulsions renders the stability issue of great importance. Moreover, the ultra-low aqueous-aqueous inner interfacial tension, the anisotropic nature of the droplets with distinct lobe composition, and the random orientation in the continuous phase endow the complicated and various demulsification mechanisms.

Batch-Scale Preparation of Reverse Janus Emulsions.

A strategy is proposed to produce novel (W1 + W2)/O reverse Janus emulsions in batch scale simply by one-step vortex mixing. Aqueous two-phase systems (ATPSs), i.e.