Integrated analysis of lncRNA, miRNA and mRNA expression profiles reveals regulatory pathways associated with pig testis function.

Long noncoding RNA (lncRNA) and microRNA (miRNA) are known to play pivotal roles in mammalian testicular function and spermatogenesis. However, their impact on porcine male reproduction has yet to be well unraveled. Here, we sequenced and identified lncRNA and miRNA expressed in the testes of Chinese indigenous Banna mini-pig inbred line (BMI) and introduced Western Duroc (DU) and Large White (LW) pigs.

Late Magmatic Activity in the Tarim Large Igneous Province: Evidence from the Puchang Granites.

The magmatic evolution sequence and tectonic activity background of the Permian Tarim large igneous province were discussed based on the research of petrogeochemistry, chronology, and zircon Lu-Hf isotope of two types of granite distributed in the Puchang V-Ti-magnetite deposit in the northwestern margin of Tarim. The results show that the two types are characterized by high silica, alkali, and potassium content. Both types of granites are enriched with light rare earth elements, including porphyritic granites with a negative anomaly of Eu and biotite monzogranites with a weak positive anomaly of Eu.

Long- and short-read RNA sequencing from five reproductive organs of boar.

The production of semen in boars involves multiple reproductive glands, including the testis (Tes), epididymis (Epi), vesicular gland (VG), prostate gland (PG), and bulbourethral gland (BG). However, previous studies on boar reproduction primarily focused on the testis, with little attention paid to the other glands. Here, we integrated single-molecule long-read sequencing with short-read sequencing to characterize the RNA landscape from five glands of Banna mini-pig inbred line (BMI) and Diannan small-ear pigs (DSE).

Molecular characteristics and transcriptional regulatory of spermatogenesis-related gene in adult Banna mini-pig inbred line (BMI).

plays critical roles in mammalian spermatogenesis and cilium maturation. Here, the testes of 12-month-old adult boars of Banna mini-pig inbred line (BMI) were subjected to whole-transcriptome sequencing. The results indicated that the average expression (raw count) of gene in BMI testes was 16138.

Transcriptomic analysis of testis and epididymis tissues from Banna mini-pig inbred line boars with single-molecule long-read sequencing†.

In mammals, testis and epididymis are critical components of the male reproductive system for androgen production, spermatogenesis, sperm transportation, as well as sperm maturation. Here, we report single-molecule real-time sequencing data from the testis and epididymis of the Banna mini-pig inbred line (BMI), a promising laboratory animal for medical research. We obtained high-quality full-length transcriptomes and identified 9879 isoforms and 8761 isoforms in the BMI testis and epididymis, respectively.

, a gene specifically expressed in the testis and seminal vesicle of the Banna mini-pig inbred line (BMI).

Septin14 is an important spermatogenesis related gene involved in the pathogenesis of male infertility that has not been well studied. Here, full-length Septin14 cDNA of the Banna mini-pig inbred line (BMI) was cloned using the RACE method and expressed in pig kidney epithelial cells (PK15) and Rosetta (DE3) cells. Septin14 expression was identified in somatic tissues and testis in different developmental stages.