Publications by authors named "Hailian Zang"

Blue honeysuckle (Lonicera caerulea L.) has been widely used in food, medicine, health products, cosmetics, materials, and other products. Between September 2022 and September 2023, a leaf spot disease was observed on approximately 20% of blue honeysuckle plants of the 'Lanjingling' cultivar grown in a 0.

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  • The symptoms of the disease start with small brown leaf spots that enlarge over time, and researchers collected samples to analyze the causative agent, revealing the presence of Fusarium species.
  • The study utilized molecular verification techniques, including PCR amplification, and confirmed the identity of the isolated fungi as Fusarium asiaticum, based on high sequence similarity in multiple genes across samples.
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  • Symptoms included black spots on leaves, prompting a study to identify the cause through fungal isolation from infected samples.
  • Fungal colonies consistent with Cladosporium species were found on all symptomatic leaves, confirmed via PCR analysis, indicating a specific fungal infection responsible for the leaf spots.
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Triclocarban (TCC) and its metabolite, 3,4-dichloroaniline (DCA), are classified as emerging organic contaminants (EOCs). Significant concerns arise from water and soil contamination with TCC and its metabolites. These concerns are especially pronounced at high concentrations of up to approximately 20 mg/kg dry weight, as observed in wastewater treatment plants (WWTPs).

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  • Interest in blue honeysuckle (Lonicera caerulea L.) for horticulture and medicinal purposes has increased recently, with observations of a leaf spot disease affecting about 20% of 'Lanjingling' plants in Heilongjiang Province, China, between September 2022 and September 2023.
  • The disease caused black leaf spots that eventually covered entire leaves, and infected tissue samples were treated and cultured to isolate the fungus, resulting in the identification of Neopestalotiopsis rosae through both morphological and molecular methods.
  • The study used specific PCR primers for genomic analysis, confirming high sequence identity with known N. rosae sequences, indicating a strong link between the observed symptoms and the identified pathogen.
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  • Blue honeysuckle cultivation in China is growing, but leaf spot disease is affecting around 30% of 'Lanjingling' plants in a specific field, leading to significant leaf damage and loss.
  • Researchers isolated five fungal pathogens from the infected plants, identified as Pseudopithomyces species based on their unique morphological characteristics observed in the lab.
  • Molecular analysis of one isolate confirmed its identity with high genetic similarity to known Pseudopithomyces chartarum sequences, aiding in the understanding of the disease's causative agents.
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Triclocarban (TCC), an emerging organic contaminant, poses a potential threat to human health with long-term exposure. Here, Rhodococcus rhodochrous BX2 and Pseudomonas sp. LY-1 were utilized to degrade TCC at environmental related concentrations for enhancing TCC biodegradation and investigating whether the toxicity of intermediate metabolites is lower than that of the parent compound.

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Blue honeysuckle (Lonicera caerulea L.) fruit is growing in popularity as a natural, functional 'super fruit', but its storage is challenged by pathogen infection. In June 2022, approximately 30% of 100 kg of blue honeysuckle fruits (cv.

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  • China leads the world in cultivating blue honeysuckle (Lonicera caerulea L.), with a notable case of leaf spots affecting 10% of plants in Harbin, Heilongjiang Province observed in June 2022.
  • The affected leaves showed symptoms like chlorosis and brown margins, prompting a study where common laboratory techniques were applied to isolate the responsible pathogen from infected leaves.
  • Genetic analysis revealed that the pathogen, identified as Didymella glomerata, had a high genetic similarity to previously recorded strains based on multiple gene sequences, confirming its identity through both morphological and molecular methods.
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Blue honeysuckle (Lonicera caerulea L.) is a perennial plant of the Caprifoliaceae family and Lonicera genus, the largest genus in the plant kingdom. Between September 2021 and September 2022, a leaf spot disease was observed on ~20% of blue honeysuckles of the 'Lanjingling' cultivar grown in a 3.

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The simultaneous bioremediation and bioconversion of papermaking wastewater by psychrotrophic microorganisms holds great promise for developing sustainable environments and economies in cold regions. Here, the psychrotrophic bacterium Raoultella terrigena HC6 presented high endoglucanase (26.3 U/mL), xylosidase (732 U/mL), and laccase (8.

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  • Triclocarban (TCC) is a harmful organic contaminant that poses a threat to soil health and microbial communities, prompting the need for effective remediation strategies.
  • Researchers focused on a TCC-degrading strain (BX2 and LY-1, called TC1) immobilized on biochar to enhance TCC removal from contaminated soil and found a significant improvement in degradation efficiency.
  • High-throughput sequencing revealed important microbial interactions and enzymes involved in TCC degradation, suggesting that this approach could be a valuable method for sustainable soil bioremediation.
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Global-scale estrone (E1) contamination of soil and aquatic environments results from the widespread use of animal manure as fertilizer, threatening both human health and environmental security. A detailed understanding of the degradation of E1 by microorganisms and the associated catabolic mechanism remains a key challenge for the bioremediation of E1-contaminated soil. Here, Microbacterium oxydans ML-6, isolated from estrogen-contaminated soil, was shown to efficiently degrade E1.

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The key to the efficient removal of pulping wastewater lies in the effective degradation of lignin at high temperature. There is thus an urgent need to seek effective eco-environmental techniques to overcome this environmental limit for lignin degradation. The soil isolate thermophilic Serratia sp.

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In this study, accumulated fermentable sugars from biosaccharified corn straw were used to generate methane through anaerobic digestion (AD). The results showed that reducing sugars from biosaccharification expanded corn straw (BECS) treated with Clostridium thermocellum XF811 accumulated with yields of 94.9 mg/g.

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In the cold regions of China, lignin-rich corn straw accumulates at high levels due to low temperatures. The application of psychrotrophic lignin-degrading bacteria should be an effective means of overcoming the low-temperature limit for lignin degradation and promoting the utilization of corn straw. However, this application is limited by the lack of suitable strains for decomposition of lignin; furthermore, the metabolic mechanism of psychrotrophic lignin-degrading bacteria is unclear.

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The microbial biofilm adheres to the surface of the carrier, which protects the pollutant-degrading bacteria and resists harsh environments; thus, research on biofilm-forming patterns will help promote the application of biofilms in wastewater treatment. Herein, univariate analysis and response surface methodology (RSM) confirmed that glucose and mannose at 3-5 g/L promoted biofilm formation. Notably, the microplate method demonstrated that compared to trivalent cations, divalent cations could more greatly enhance the activity (especially magnesium) of the biofilm matrix, and the period of biofilm formation in the three strains was divided into the following stages: initial attachment (0-10 h), microcolony (10-24 h), maturation (24-48 h), and dispersion (36-72 h).

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  • A psychrotrophic bacterium, Arthrobacter sp. C2, was used for the first time to effectively treat black liquor from pulp and paper mills at low temperatures.
  • The strain demonstrated a lignin degradation rate of 65.5% and significant removal of color and chemical pollutants within 15 days at 15 °C.
  • Cold shock protein 1 (Csp1) was found to regulate both cold adaptation and the activity of dye-decolorizing peroxidase (DyP), contributing to lignin breakdown and providing a potential method for bioremediation in cold climates.
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Low-temperature biorefineries inhibit the multiplication of undesired microorganisms, improve product purity and reduce economic costs. Herein, to improve the 2,3-butanediol (2,3-BD) bioconversion efficiency from hemicellulose, a psychrotrophic hemicellulose-degrading strain Raoultella terrigena HC6 with high β-xylosidase activity 1520 U/mL was isolated and genetically modified. Xylan (hemicellulose replacement) was depolymerized into xylooligosaccharides (XOS) and xylose by HC6, which were further converted into 2,3-BD.

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  • - In September 2021, a leaf spot disease affecting about 10% of blue honeysuckle 'Lanjingling' was observed in a field in Dandong City, China, with symptoms including black mildew spots and yellow halos on leaves.
  • - Small leaf samples were cultured, resulting in six purified strains of the fungus, identified as Alternaria alternata based on morphological characteristics and measurements.
  • - Molecular analysis confirmed the identity of the fungus through gene amplification and sequencing, leading to a greenhouse experiment that replicated the disease symptoms on healthy plants when exposed to the fungal spores.
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Triclocarban (TCC) is an emerging and intractable environmental contaminant due to its hydrophobicity and chemical stability. However, the antibacterial property of TCC limits its biodegradation, and only the functional enzyme TccA involved in TCC degradation has been characterized to date. In this study, we report a highly efficient TCC-degrading bacterium, Rhodococcus rhodochrous BX2, that could degrade and mineralize TCC (10 mg/L) by 76.

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Vanillin is a natural flavoring agent that is widely used in the bioengineering industry. To enable sustainable development, joint consideration of bacterial performance and negative environmental impacts are critical to vanillin biosynthesis. In this study, a cold shock protein (csp) gene was upregulated for maintaining stable growth in Arthrobacter sp.

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To investigate the efficiency of psychrotrophic cellulose-degrading fungal strains (PCDFSs) and estrogen-degrading bacteria (EDBs) combined with humic acid (HA) on estrone (E1) and 17-β-estradiol (E2) degradation, five compost groups (T, HA, EDB, PCDFS, and CK) were prepared and composted for 32 days at 11-14°C. The results indicated that inoculation increased the temperature to 62.2°C and promoted E1 degradation to the lowest level of 100.

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In recent years, special attention has been devoted to biodemulsifiers as a new type of environment-friendly demulsifiers. A novel biodemulsifying oxalate decarboxylase (OxdC) secreted by Bacillus mojavensis XH1 is reported in the present study. A genome-wide comparison showed that strains with high demulsification efficiencies all possess alkane degradation genes.

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  • The study looked at how certain cold-loving fungi helped break down pig manure compost to make it better at low temperatures.
  • Three types of fungi were found and used, making the compost heat up quickly and stay warm longer than normal compost.
  • The compost ended up being a good fertilizer because of these fungi, which improved the overall quality of the compost while it was decomposing.
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