Young Goji Fruit Volatiles Regulate the Oviposition Behavior and Chemosensory Gene Expression of Gravid Female .

The goji fruit fly, , is a major pest on the well-known medicinal plant . Dissecting the molecular mechanisms of the oviposition selection of regarding the host plant will help to identify new strategies for pest fly control. However, the molecular mechanism of chemical communication between the goji fruit fly and the host goji remains unclear.

Prediction of Potential Distribution of in China under the Current and Future Climate Change.

is an important pest of , distributed in China, Korea, and Japan. In recent years, its damage to has become increasingly serious, causing enormous economic losses in China. This study and prediction of current and future suitable habitats for in China can provide an important reference for the monitoring, early warning, prevention, and control of the pest.

Selection and Validation of Reference Genes for Gene Expression in Loginova under Different Insecticide Stresses.

Insecticide resistance has long been a problem in crop pest control. is a major pest on the well-known medicinal plants L. Investigating insecticide resistance mechanisms of will help to identify pesticide reduction strategies to control the pest.

Feeding-induced plant metabolite responses to a phoretic gall mite, its carrier psyllid and both, after detachment.

Phoresy is one of the most distinctive relationships between mites and insects, and the off-host interaction between phoretic mites and their carriers is the most critical factor sustaining the phoretic association. As phoretic associations commonly occur in temporary habitats, little is known about off-host interactions between phoronts and carriers. However, an off-host interaction has been reported, in which the plant-mediated competition between a phoretic gall mite, Aceria pallida, and its psyllid vector, Bactericera gobica, after detachment decreases leaf abscission caused by B.

Expression Pattern and Ligand Binding Characteristics Analysis of Chemosensory Protein SnitCSP2 from .

is an important wood-boring wasp to conifers native to Asia, causing considerable economic and ecological damage. However, the current control means cannot achieve better efficiency, and it is expected to clarify the molecular mechanism of protein-ligand binding for effective pest control. This study analyzed the expression pattern of CSP2 in (SnitCSP2) and its features of binding to the screened ligands using molecular docking and dynamic simulations.

Investigating SnocCSP4 expression and key compound interactions with SnocOBP4 in Sirex noctilio Fabricius (Hymenoptera: Siricidae).

Sirex noctilio, a significant pest impacting Pinus sylvestris var. mongolica, presents control difficulties due to its wood-boring behavior, paucity of natural antagonists, and wide-ranging habitats. Our research aims to elucidate the functionality and operational mechanisms of chemosensory proteins 4 (SnocCSP4), providing strategic insights for pest management and fostering further exploration in CSPs.

Integrated Transcriptome and Metabolome Dynamic Analysis of Galls Induced by the Gall Mite on Reveals the Molecular Mechanism Underlying Gall Formation and Development.

Galls have become the best model for exploring plant-gall inducer relationships, with most studies focusing on gall-inducing insects but few on gall mites. The gall mite is a major pest of wolfberry, usually inducing galls on its leaves. For a better understanding of gall mite growth and development, the dynamics of the morphological and molecular characteristics and phytohormones of galls induced by were studied by histological observation, transcriptomics and metabolomics.

Secondary Rhinaria Contribute to Major Sexual Dimorphism of Antennae in the Aphid (Takahashi).

Sexual generation is an important generation in the life cycle of host-alternating aphids, and its population size determines the intensity of the peak in the next spring. Although male trapping techniques based on olfactory stimuli have been successfully established in the field, the biological basis of olfactory perception in males is unclear. In this study, we compared the morphology of antennae and the types, sizes, numbers, and distribution of sensilla between males and sexual females in the host-alternating aphid (Hemiptera: Aphididae).

Combined Metabolome and Transcriptome Analysis Highlights the Host's Influence on Metabolite Accumulation.

The medicinal plant Ma (Orobanchaceae) is a holoparasitic angiosperm that takes life-essential materials from (C. A. Mey.

Morphology and location of sensilla in the antennae and ovipositor of Sirex noctilio (Hymenoptera: Siricidae).

The woodwasp Sirex noctilio Fabricius is a major quarantine pest that was reported in China in 2013 and mostly damages Pinus sylvestris var. mongolica. Reverse chemical ecology, which uses chemical lures to catch or block insects from mating is the classic way to control forestry pests.

Transcriptome-Based Selection and Validation of Reference Genes for Gene Expression in Goji Fruit Fly ( Becker) under Developmental Stages and Five Abiotic Stresses.

Goji fruit fly, , is a major pest on the well-known medicinal plant . Dissecting molecular mechanisms of infestation and host selection of will contribute to the determination of best management practices for pest fly control. Gene expression normalization by Real-time quantitative PCR (qPCR) requires the selection and validation of appropriate reference genes (RGs).

Mating Behavior and Identification of Male-Produced Pheromone Components in Two Woodwasps, and , in China.

To protect vulnerable trees from native and invasive wood wasps, the mating behavior of these two woodwasp species ( and , respectively) and factors influencing this behavior were investigated in cages outdoors. Male-produced pheromones were identified in both woodwasp species. Compared with the native species the invasive species showed stronger mating ability, including mating frequency, time, and duration.

Key Residues Affecting Binding Affinity of Fabricius Odorant-Binding Protein (SnocOBP9) to Aggregation Pheromone.

Fabricius (Hymenoptera Siricidae) is a major quarantine pest responsible for substantial economic losses in the pine industry. To achieve better pest control, ()-3-decen-ol was identified as the male pheromone and used as a field chemical trapping agent. However, the interactions between odorant-binding proteins (OBPs) and pheromones are poorly described.

Antennal transcriptome analysis of olfactory genes and tissue expression profiling of odorant binding proteins in Semanotus bifasciatus (cerambycidae: coleoptera).

Background: Insect olfactory proteins can transmit chemical signals in the environment that serve as the basis for foraging, mate searching, predator avoidance and oviposition selection. Semanotus bifasciatus is an important destructive borer pest, but its olfactory mechanism is not clear. We identified the chemosensory genes of S.

Morphology and distribution of antennal sensilla in the gall midge Gephyraulus lycantha (Diptera: Cecidomyiidae).

The gall midge Gephyraulus lycantha (Diptera: Cecidomyiidae) is a serious gall-forming pest that causes devastating damage in the wolfberry, Lycium barbarum (Solanaceae) in Northwest China. In the present study, the external morphology and ultrastructure of the antennae and the antennal sensilla of G. lycantha were examined by scanning electron microscopy.

Antennal transcriptome analysis of olfactory genes and characterizations of odorant binding proteins in two woodwasps, Sirex noctilio and Sirex nitobei (Hymenoptera: Siricidae).

Background: The woodwasp Sirex noctilio Fabricius is a major quarantine pest worldwide that was first discovered in China in 2013 and mainly harms Pinus sylvestris var. mongolica Litv..

Morphology and distribution of sensilla on the antennae and ovipositor of the Hymenopteran parasitoid Pseudotorymus jaapiellae Yang et Chen.

Pseudotorymus jaapiellae is an important ectoparasitoid of the larvae of Gephyraulus lycantha, a serious gall-forming pest that devastates wolfberry, Lycium barbarum, in Northwest China. To provide requisite background for our ongoing research on the mechanisms of P. jaapiellae's host location and subsequent oviposition, we used scanning electron microscopy to describe the external morphologies and distributions of sensilla on their antennae and ovipositors.

Peach volatile emission and attractiveness of different host plant volatiles blends to Cydia molesta in adjacent peach and pear orchards.

The oriental fruit moth (OFM), Cydia (= Grapholitha) molesta, is a highly damaging pest; peaches are its primary host, and pears serve as post-peach secondary hosts during the late season in China. We collected volatiles from detached peach shoots and fruits, and identified them with gas chromatography-mass spectrometry (GC-MS). Antennally active compounds were identified by gas chromatography-electroantennogram detection (GC-EAD), and these were further tested in the laboratory and field.

[Hormone response of to gall mite and effects of exogenous salicylic acid on the infestation and development of gall mite].

To investigate the hormone responses of to infestation and the effects of exogenous salicylic acid on the infestation and development of gall mite, four phytohormones (salicylic acid (SA), jasmonic acid (JA), Auxin, and abscisic acid (ABA)) of leaves infested with were determined by LC-MS/MS. Moreover, the effects of exogenous SA on the growth of gall and mite population were investigated. The results showed that contents of SA and JA in leaves infested with were significantly higher (4.

[Electrophysiological and behavioral responses of gall midge, sp. to volatiles of host plant ].

Plant-derived volatiles are important for guiding herbivorous insects to their host plants. sp. (Diptera: Cecidomyiidae) is the most serious pest of .

[Effects of root extract of host plants on seed germination and haustorium formation of ].

We examined the effects of root extracts of and in Chenopodiaceae extracted by water and ethanol on seed germination and haustorium formation of by filter paper culture dish method. The results showed that only adding root extract had no effect on seed germination and haustorium formation of . The germination rate of seeds treated by adding 10 mg·kg gibberellin to the root extracted by ethanol was not significantly different from that of the control (GA), whereas those treated by adding gibberellin to the ethanol extract of two kinds of host root was increased by more than 10 times.

[Metabolomics analysis of Lycium barbarum leaves after Aceria pallida infestation].

Aceria pallida is one of the most common pests in the main production areas of Lycium barbarum in China. The mite mainly feeds on foliage, leading to local tissue deformation and formation of massive galls, which seriously affects the growth and yield of L. barbarum.

Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle.

This report described sample preparation methods that scanning and transmission electron microscope observations, demonstrated by preparing appendages of the woodboring beetle, Chlorophorus caragana Xie & Wang (2012), for both types of electron microscopy. The scanning electron microscopy (SEM) sample preparation protocol was based on sample chemical fixation, dehydration in a series of ethanol baths, drying, and sputter-coating. By adding Tween 20 (Polyoxyethylene sorbitan laurate) to the fixative and the wash solution, the insect body surface of woodboring beetle was washed more cleanly in SEM.

[Overwintering status and species identification of gall mite in major wolfberry production areas].

To identify the wolfberry gall mite species and their overwintering situation in different wolfberry production areas and to provide a scientific basis for their prevention and control. We investigate the phoresy overwintering situation of gall mite, and identify the species of gall mite. Our results showed that the phoretic gall mite was Aceria pallida in all the production areas.

[Ultrastructure observations on antennal sensilla of Heortia vitessoides the most serious pest of Aquilaria sinensis].

Heortia vitessoides is the most serious pest of Aquilaria sinensis,which is an economically important evergreen tree native to China and is the principal source of Chinese agarwood. In severe infestations,the insects completely eat up the leaves of A. sinensis,causing severe economic losses.