Publications by authors named "Hailey N C Sedam"
Article Synopsis
- The strain JAY270/PE2 is a powerful yeast used for bioethanol production from sugarcane, known for its non-aggregation property which aids in cell recycling during fermentation.
- The strain faces potential genomic instability over time, leading to issues such as cell aggregation and defects in mother-daughter cell separation, resulting in observable rough colonies.
- Investigating these changes revealed that the instability rates in JAY270 are similar to those in conventional lab strains, highlighting that genomic dynamics in this industrial yeast strain are typical rather than excessively unstable.
Cells carrying deletions of genes encoding H-class ribonucleases display elevated rates of chromosome instability. The role of these enzymes is to remove RNA-DNA associations including persistent mRNA-DNA hybrids (R-loops) formed during transcription, and ribonucleotides incorporated into DNA during replication. RNases H1 and H2 can degrade the RNA component of R-loops, but only RNase H2 can initiate accurate ribonucleotide excision repair (RER).
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