Ubiquity and ecological risks of conjugated steroids cannot be overlooked: First evidence from estuarine sediments.

Steroids, renowned for endocrine-disrupting capabilities, have garnered significant research interest, predominantly centered on their parent forms. This study was the first to explore the composition, spatiotemporal characteristics, sources, mass inventories, and ecological risks of steroids in free and conjugated forms in estuarine sediments. Seventeen steroids were identified in sediments with the total levels of 1.

Dynamic impacts of short-term bath administration of enrofloxacin on juvenile black seabream Acanthopagrus schlegelii.

Dynamic impacts of short-term enrofloxacin (ENR) exposure on juvenile marine fish are not well understood, and the underlying mechanisms remain unclear. We therefore investigated the accumulation and elimination of ENR in the liver of juvenile black seabream Acanthopagrus schlegelii. Meanwhile, the dynamic alterations of biochemical parameters and liver transcriptomes after short-term bath immersion and withdrawal treatment were explored.

Distribution and typologies of anthropogenic seafloor litter in the Pearl River Estuary and adjacent coastal waters, China.

Marine litter pollution poses a significant threat to offshore ecosystems, eliciting widespread concern. We investigated seafloor litter patterns in the Pearl River Estuary and adjacent coastal waters of China in 2023 via bottom trawl survey. Average number and weight densities were found to be 154.

Whole-Genome Identification and Characterization of the DKK Gene Family and Its Transcription Profiles: An Analysis of the Chinese Soft-Shell Turtle ().

The DKK family is a canonical small family of WNT antagonists. Though recent studies have suggested that the DKK gene family may be involved in sex differentiation in , there are still a lot of things about the DKK gene family that we do not know. In this study, we used bioinformatics methods to identify members of the DKK gene family in and analyzed their phylogeny, covariance, gene structure, structural domains, promoter conserved sites, signal peptides, gonadal transcription factors, transcriptional profiles, and tissue expression profiles.

Identification of Sex-Specific Markers and Candidate Genes Using WGS Sequencing Reveals a ZW-Type Sex-Determination System in the Chinese Soft-Shell Turtle ().

Male and female Chinese soft-shelled turtles () have sex-dimorphic growth patterns, and males have higher commercial value because of their larger size and thicker calipash. Thus, developing sex-specific markers is beneficial to studies on all-male breeding in . Here, we developed an accurate and efficient workflow for the screening of sex-specific sequences with ZW or XY sex determination systems.

Comparative genomic survey and functional analysis of DKKL1 during spermatogenesis in the Chinese soft-shelled turtle (Pelodiscus sinensis).

A feature of the Chinese soft-shelled turtle (Pelodiscus sinensis) is seasonal spermatogenesis; however, the underlying molecular mechanism is not well clarified. Here, we firstly cloned and characterized P. sinensis DKKL1, and then performed comparative genomic studies, expression analysis, and functional validation.

Sex-specific metabolic dysregulation in digestive glands of green mussels following exposure to triazophos.

As a ubiquitous environmental pollutant in China, triazophos (TP) is known to have neurotoxicity, oxidative stress, and reproductive toxicity to mussels. To investigate the molecular mechanisms of TP toxicity, metabolic changes in the digestive glands of Perna viridis in different sexes were examined after treated with 35 μg/L TP. Notably, 158 significant different metabolites (SDMs) were detected in TP-treated mussels and more than half of the SDMs were lipids and lipid-like molecules, which suggested that TP disturbed the lipid metabolism of P.

Source apportionment of nitrate in the Pearl River Estuary using δN and δO values and isotope mixing model.

The mitigation of eutrophication in the Pearl River Estuary (PRE) has encountered numerous challenges in regards to source control. Herein, the isotope mixing model (SIAR) was used to quantify the primary nitrate sources in the PRE. The results showed that the nitrate levels were significantly higher in the high-flow season than in the low-flow season.

Genome-wide identification, evolution and expression analysis of bone morphogenetic protein (BMP) gene family in chinese soft-shell turtle ().

Bone morphogenetic proteins (BMPs) play a crucial role in bone formation and differentiation. Recent RNA-Seq results suggest that BMPs may be involved in the sex differentiation of , yet more relevant studies about BMPs in are lacking. Herein, we identified BMP gene family members, analyzed the phylogeny, collinear relationship, scaffold localization, gene structures, protein structures, transcription factors and dimorphic expression by using bioinformatic methods based on genomic and transcriptomic data of .

Expression and Characterization of the Gene and Its Response to E2/MT Treatment in the Chinese Soft-Shelled Turtle ().

Spats1 (spermatogenesis-associated, serinerich 1) has been characterized as a male-biased gene which acts an important role in the germ cell differentiation of mammals. Nevertheless, the function of Spats1 in the Chinese soft-shelled turtle (P. sinensis) has not yet been reported.

Whole-Transcriptome Analysis Identifies Gender Dimorphic Expressions of Mrnas and Non-Coding Rnas in Chinese Soft-Shell Turtle ().

In aquaculture, the Chinese soft-shelled turtle () is an economically important species with remarkable gender dimorphism in its growth patterns. However, the underlying molecular mechanisms of this phenomenon have not been elucidated well. Here, we conducted a whole-transcriptome analysis of the female and male gonads of .

[Population characteristics of in the Pearl River Estuary during 2017 and 2020].

To understand the population characteristics of , an important economic fish in the Pearl River Estuary, the biological characteristics and resource density distribution characteristics of were preliminarily analyzed using bottom trawling by cruises conducted in each spring and autumn during 2017 and 2020. The results showed that the body length and weight of ranged between 22-168 mm and 0.23-103.

Chromosome-level genome assembly of Asian yellow pond turtle (Mauremys mutica) with temperature-dependent sex determination system.

Knowledge of sex determination has important implications in physiology, ecology and genetics, but the evolutionary mechanisms of sex determination systems in turtles have not been fully elucidated, due to a lack of reference genomes. Here, we generate a high-quality genome assembly of Asian yellow pond turtle (Mauremys mutica) using continuous long-read (PacBio platform), Illumina, and high-throughput chromatin conformation capture (Hi-C) technologies. The M.

Tetrabromobisphenol A and hexabromocyclododecanes in sediments from fishing ports along the coast of South China: Occurrence, distribution and ecological risk.

Tetrabromobisphenol A (TBBPA) and hexabromocyclododecanes (HBCDDs) have attracted extensive attention due to their strong persistence and toxicity. However, little has been known about their pollution status in fishing ports, which are typical sinks of land-sourced pollutants. In this study, we investigated the occurrence, distribution and ecological risk of TBBPA and HBCDDs in sediments from fishing ports along the coast of South China.

Identification and functional analysis of miRNAs in skeletal muscle of juvenile and adult largemouth bass, Micropterus salmoides.

MicroRNAs (miRNAs) are considered key regulators to post-transcriptionally regulate gene expression affecting multiple biological activities. However, the developmental process of fish skeletal muscles is regulated by complicated molecular mechanism that has not been completely well-described. In this study, two small RNAs libraries from skeletal muscle of juvenile as well as adult largemouth bass (LMB) were obtained and sequenced using deep sequencing to investigate the development-related miRNAs.

Transcriptome analysis reveals sex-specific alterations in gonads of green mussel exposed to organophosphorus insecticide triazophos.

Triazophos (TP) is a widespread pollutant in aquatic environments. A sex-specific metabolic response in green-lipped mussel Perna viridis to TP exposure was observed in our previous study, and this led us to investigate the mechanisms associated with its toxicity. P.

Oyster arsenic, cadmium, copper, mercury, lead and zinc levels in the northern South China Sea: long-term spatiotemporal distributions, combined effects, and risk assessment to human health.

Estuarine and coastal ecosystems are often considered vulnerable due to the complex biogeochemical processes and the human disturbances through a variety of pollution. Among environmental contaminants, heavy metals in estuarine and coastal ecosystems have been of increasing concern in environmental conservation. Long-term exposure to heavy metal contamination, mainly through food and water, could be harmful to human health.

The complete mitochondrial genome of (Sauvage 1878) (Siluriformes, Pangasiidae).

The striped catfish, (Sauvage 1878), belongs to the family Pangasiidae, is an important economic freshwater species. We determined the complete mitogenome of the through sanger method. The complete mitochondrial genome of was a circular molecule of 16,469 bp with a total GC content of 44% which contains 37 genes, including 13 protein-coding genes, 2 rRNA genes, and 22 tRNAs genes.

Contamination and ecological risks of steroid metabolites require more attention in the environment: Evidence from the fishing ports.

Due to strong endocrine disrupting effects, steroids in the environment have attracted substantial attention, with studies mostly focusing on the parent steroids. Here, we conducted the first investigation on the contamination profiles, possible sources, mass inventories, and ecological risks of 27 steroids and their metabolites in 15 typical fishing ports in Southeast China. Twelve steroids were detectable in the sediment samples with the total mean concentrations of 4.

Complete mitochondrial genome and the phylogenetic position of the Burmese narrow-headed softshell turtle (Testudines: Trionychidae).

Narrow-headed softshell turtles constitute a group of critically endangered freshwater turtles that belong to the family Trionychidae. Here, we determine the complete mitogenome of the Burmese narrow-headed softshell turtle . The length of the mitochondrial genome was 16,614 bp, composed of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and twelve noncoding regions.

Sex-specific effects of triphenyltin chloride (TPT) on thyroid disruption and metabolizing enzymes in adult zebrafish (Danio rerio).

Although organotin compounds are known to disturb thyroid signaling and antioxidant defense system, the sex-differences underlying these effects of triphenyltin chloride (TPT) in fish remain unclear. To understand these differences, adult zebrafish (Danio rerio) were exposed to different concentrations of TPT (0, 10, 100, or 1000 ng/L) for 28 days. Female zebrafish exposed to TPT showed significantly increased thyroxine (T4) content and decrease triiodothyronine (T3) content, possibly due to downregulation of deiodinase (dio2) and uridine diphosphate glucuronosyl transferase (ugt1ab).

Oyster copper levels in the northern South China Sea from 1989 to 2015: spatiotemporal trend detection and human health implications.

Coastal heavy metal pollution has become an important topic for seafood safety and marine environmental protection. Unlike toxic heavy metals such as cadmium or chromium, copper is essential for oysters' growth but can inhibit their immune response to exotic stress when going above normal levels. Oysters with high copper levels can easily accumulate and transfer abnormal amounts of copper to upper trophic levels, and generate health risks for humans.

Effects of di-n-butyl phthalate on gills- and liver-specific EROD activities and CYP1A levels in juvenile red snapper (Lutjanus argentimaculatus).

Di-n-butyl phthalate, one of the most easily detected pollutants of phthalate esters in the environment, has been added to the priority list of hazardous substances by many countries. As one of low molecular weight phthalates, Di-n-butyl phthalate may have a great adverse potency on various aquatic organisms. In this study, the juvenile red snapper, Lutjanus argentimaculatus, was exposed to the concentrations of Di-n-butyl phthalate (20 μg L, 100 μg L and 500 μg L) for 15 days.

Transcriptome analysis of acute exposure of the Manila clam, Ruditapes philippinarum to perfluorooctane sulfonate (PFOS).

Perfluorooctane sulfonate (PFOS) is an increasingly important environmental pollutant, which has been detected almost everywhere in the environment. Despite the widespread presence of PFOS, much less notice is taken of its toxicology effects on marine bivalves. Thus, the transcriptome response to PFOS treatment (nominal concentration of 20 mg/L) in hepatopancreas of a sentinel organism, Ruditapes philippinarum was examined.

Transcriptomic Changes in Liver of Juvenile Cynoglossus semilaevis following Perfluorooctane Sulfonate Exposure.

Perfluorooctane sulfonate (PFOS) is an increasingly important environmental pollutant that is pervasive in the environment. A number of studies have focused on the toxicological effects of PFOS on model fish species (zebrafish and medaka), but little is known about the impact of PFOS on commercially important marine fish. Thus, the present study examined transcriptome responses to PFOS exposure in the liver of juvenile Cynoglossus semilaevis, an important farmed flatfish in China.