From bioinformatics to clinical applications: a novel prognostic model of cuproptosis-related genes based on single-cell RNA sequencing data in hepatocellular carcinoma.

Objective And Methods: To ascertain the connection between cuproptosis-related genes (CRGs) and the prognosis of hepatocellular carcinoma (HCC) via single-cell RNA sequencing (scRNA-seq) and RNA sequencing (RNA-seq) data, relevant data were downloaded from the GEO and TCGA databases. The differentially expressed CRGs (DE-CRGs) were filtered by the overlaps in differentially expressed genes (DEGs) between HCC patients and normal controls (NCs) in the scRNA-seq database, DE-CRGs between high- and low-CRG-activity cells, and DEGs between HCC patients and NCs in the TCGA database.

Results: Thirty-three DE-CRGs in HCC were identified.

Determination of azithromycin residue in pork using a molecularly imprinted monolithic microcolumn coupled to liquid chromatography with tandem mass spectrometry.

Using spiramycin as a dummy template, a molecularly imprinted polymer monolithic micro-column with high selection to azithromycin was prepared in a micropipette tip. The imprinting factor of the monolithic micro-column prepared was approximately 2.67 and the morphological structure of the polymers was characterized by scanning electron microscopy.

Determination of 26 veterinary antibiotics residues in water matrices by lyophilization in combination with LC-MS/MS.

A sensitive, simple and reliable multi-residue method was developed for the determination of 26 widely used veterinary antibiotics including 6 macrolides, 2 pleuromutilins, 4 tetracyclines, 2 lincosamides, 6 fluoroquinolones and 6 sulfonamides in different water matrices using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Water samples were lyophilized to dryness. Target compounds were separated on Zorbax SB-Aq column (150mm×2.

Simultaneous determination of major type-B trichothecenes and the de-epoxy metabolite of deoxynivalenol in chicken tissues by HPLC-MS/MS.

In this study, a specific and sensitive LC-MS/MS method for the simultaneous analysis of type-B trichothecenes (deoxynivalenol, 3-acetyldeoxynivalenol, and 15-acetyldeoxynivalenol) and the de-epoxy metabolite of deoxynivalenol (de-epoxy-deoxynivalenol) in chicken muscle, liver, kidney, and fat tissues was developed and validated. The method involved an extraction step using ethyl acetate, followed by the evaporation of the supernatant, which was further purified by an Oasis HLB SPE cartridge (Waters, Milford, MA, USA). Chromatographic separation was performed on a C18 column by detection with MS in multiple-reaction monitoring mode and using a gradient elution program with 0.