Rapid screening of the novel bioactive peptides with notable α-glucosidase inhibitory activity by UF-LC-MS/MS combined with three-AI-tool from black beans.

This work aimed to propose a rapid method to screen the bioactive peptides with anti-α-glucosidase activity instead of traditional multiple laborious purification and identification procedures. 242 peptides binding to α-glycosidase were quickly screened and identified by bio-affinity ultrafiltration combined with LC-MS/MS from the double enzymatic hydrolysate of black beans. Top three peptides with notable anti-α-glucosidase activity, NNNPFKF, RADLPGVK and FLKEAFGV were further rapidly screened and ranked by the three artificial intelligence tools (three-AI-tool) BIOPEP database, PeptideRanker and molecular docking from the 242 peptides.

Teratogenicity of 30 nm Aluminum Oxide Nanoparticles (AlONPs) in Rats by Gavage.

Aluminum oxide nanoparticles (AlONPs) are one class of widely used nanomaterials. However, the teratogenicity toxicity of AlONPs in mammal remains poorly understood. This study was aimed to evaluate the teratogenicity of AlONPs in Sprague Dawley (SD) rats by gavage and to compare the effects of AlONPs to those of equivalent dose of microscale aluminum oxide (bulk AlO).

Biocontrol mechanism of Myxococcus xanthus B25-I-1 against Phytophthora infestans.

Phytophthora infestans is the pathogen causing potato late blight, one of the most serious diseases of potato. Myxobacteria have become a valuable biological control resource due to their preponderant abilities to produce various secondary metabolites with novel structure and remarkable biological activity. In this study, Myxococcus xanthus strain B25-I-1, which exhibited strong antagonistic activity against P.

Simple and inexpensive long-term preservation methods for Phytophthora infestans.

Phytophthora infestans is one of the most notorious pathogen among Phytophthora species causing potato late blight disease. Stable and long-term preservation of this pathogen is essential for biological research and fungicide screening. The aim of this study was to find a suitable long-term preservation method for P.

Establishment of the straightforward electro-transformation system for Phytophthora infestans and its comparison with the improved PEG/CaCl₂ transformation.

Phytophthora infestans is the most devastating pathogen of potato. For the biology study of P. infestans at the molecular level, one of the difficulties is the technique for the genetic transformation.