Establishment and evaluation of a PRRSV-sensitive porcine endometrial epithelial cell line by transfecting SV40 large T antigen.

Background: PRRSV is an infectious illness causing lung injury and abortion in sows. Cells apoptosis in the interface between the endometrium and fetal placenta is a crucial factor causing abortion. Previous study confirmed PRRSV could cause apoptosis of macrophages but rarely produced an obvious change in porcine endometrial epithelial cells (PECs).

[Treatment of greater trochanter fracture after total hip replacement].

Objective: To explore the treatment of greater trochanter fracture after total hip replacement (THR).

Methods: This series consisted of 3 males and 8 females with fractures of greater trochanter after total or partial hip replacement from 2004 to 2009. The mean age at the time of fracture diagnosis was 69 years old (range: 52 - 79).

[Isolation and identification of a thermophilic anaerobic bacterium].

Objective: To find new microbial resources from a high-temperature oil reservoir.

Methods: Strain HL-3 was isolated by Hungate Anaerobic Technique from oil reservoir water sampled from Dagang oilfield, China. Through physiological, biochemical and phylogenetic analysis, the strain HL-3 was classified.

[Identification and metabolism characterization of a Clostridium lituseburense strain isolated from high-altitude soil].

Objective: We studied the physiological, biochemical properties and metabolism of Clostridium lituseburense P4-1 from soil in Namucuo.

Methods: We adopted Hungate anaerobic technique to get Strain P4-1 from soil in Namucuo. Through physiological, biochemical and phylogenetic analysis, we identified the strain P4-1.

[Isolation and identification of a methanogen from the high temperature oil reservoir water].

To explore new microbial resources in deep subsurface oil reservoirs, strain DL-7 was isolated with Hungate technology from oil reservoir water sampled from Dagang oilfield, China. Physiological and biochemical examinations showed that H2/CO2 is the unique substrate of the strain, which cannot metabolize formate, methanol, trimethylamine, acetate and other secondary alcohols. The optimum growth conditions were further identified to be 60 degrees C, pH 7.