A colorimetric nano-enzyme assay with Ni@Pt nanoparticles as signal labels for rapid and sensitive detection of exosomal Aβ42 in plasma.

A nano-enzyme sandwich assay (SWzyme assay), a colorimetric system based on a biochip and inorganic nano-enzyme for rapid and simple determination of exosomal Aβ42 in plasma is proposed. Anti-CD63 antibody-modified biochips were prepared for plasma exosome capture and synthesized highly catalytic Ni@Pt nanozymes for detecting exosomal Aβ42. The method was able to detect exosomal Aβ42 with a limit of detection (LOD) as low as 4.

Genome-wide analysis of MYB transcription factor family and AsMYB1R subfamily contribution to ROS homeostasis regulation in Avena sativa under PEG-induced drought stress.

Background: The myeloblastosis (MYB) transcription factor (TF) family is one of the largest and most important TF families in plants, playing an important role in a life cycle and abiotic stress.

Results: In this study, 268 Avena sativa MYB (AsMYB) TFs from Avena sativa were identified and named according to their order of location on the chromosomes, respectively. Phylogenetic analysis of the AsMYB and Arabidopsis MYB proteins were performed to determine their homology, the AsMYB1R proteins were classified into 5 subgroups, and the AsMYB2R proteins were classified into 34 subgroups.

Multi-modal Ca nanogenerator via reversing T cell exhaustion for enhanced chemo-immunotherapy.

Chemo-immunotherapy holds the advantage of specific antitumor effects by activating cytotoxic lymphocyte cells (CTLs) immune response. However, multiple barriers have limited the outcomes partly due to tumor-cell-mediated exhaustion of CTLs in the immunosuppressive tumor microenvironment (iTME). Here, we rationally designed a simple-yet-versatile Ca nanogenerator to modulate iTME for enhancing 2-deoxyglucose (2-DG) mediated chemo-immunotherapy.

MYB2 and MYB108 regulate lateral root development by interacting with LBD29 in Arabidopsis thaliana.

AUXIN RESPONSE FACTOR 7 (ARF7)-mediated auxin signaling plays a key role in lateral root (LR) development by regulating downstream LATERAL ORGAN BOUNDARIES DOMAIN (LBD) transcription factor genes, including LBD16, LBD18, and LBD29. LBD proteins are believed to regulate the transcription of downstream genes as homodimers or heterodimers. However, whether LBD29 forms dimers with other proteins to regulate LR development remains unknown.

Intestinal microflora promotes Th2-mediated immunity through NLRP3 in damp and heat environments.

Background: With the worsening of the greenhouse effect, the correlation between the damp-heat environment (DH) and the incidence of various diseases has gained increasing attention. Previous studies have demonstrated that DH can lead to intestinal disorders, enteritis, and an up-regulation of NOD-like receptor protein 3 (NLRP3). However, the mechanism of NLRP3 in this process remains unclear.

Aptamer-Functionalized Interface Nanopores Enable Amino Acid-Specific Peptide Detection.

Single-molecule proteomics based on nanopore technology has made significant advances in recent years. However, to achieve nanopore sensing with single amino acid resolution, several bottlenecks must be tackled: controlling nanopore sizes with nanoscale precision and slowing molecular translocation events. Herein, we address these challenges by integrating amino acid-specific DNA aptamers into interface nanopores with dynamically tunable pore sizes.

Development and validation of a nomogram for predicting the risk of poor prognosis in patients with cerebral infarction.

Objective: To identify factors related to poor prognosis in patients with cerebral infarction (CI) and to construct and validate a personalized prediction model based on these factors.

Methods: A retrospective analysis was conducted on the clinical and follow-up data of 857 patients with CI who were diagnosed in the neurology department of a tertiary A hospital in Anhui Province, China from April 2020 to March 2022. Based on follow-up data and the Modified Rankin Scale (mRS) score one year after discharge, patients were divided into a good prognosis group (793 cases, mRS ≤2) and a poor prognosis group (64 cases, mRS >2).

Distinct microbiome of tongue coating and gut in type 2 diabetes with yellow tongue coating.

The gut microbiome plays a critical role in the pathogenesis of type 2 diabetes mellitus (T2DM). However, the inconvenience of obtaining fecal samples hinders the clinical application of gut microbiome analysis. In this study, we hypothesized that tongue coating color is associated with the severity of T2DM.

The effect of aspartame on accelerating caspase-dependent apoptosis of pancreatic islet via ZIPK/STAT3/caspase 3 signaling pathway.

Aspartame (ASP) as an important sugar substitute is widely used in pharmaceutical and food processing. Here, we compared the effects of ASP and sucrose on mice pancreatic islet cells in vivo and observed that ASP with the condition of high concentration and long-term exposure (HASP) could cause insulin secretion (500 mg/kg for 1 month). Next, we conducted iTRAQ mass spectrometry to profile the global phosphoproteome and found that phosphorylation of zipper-interacting protein kinase (ZIPK) in murine pancreatic islet tissues were induced at Thr197, Thr242, Thr282, and Ser328 by high-sucrose (HS) treatment, but only induced at Thr197 and Ser328 by HASP treatment.

Pressure-Driven Structural and Electronic Transitions in a Two-Dimensional Janus WSSe Crystal.

In this work, we presented the first report on the high-pressure structural stability and electrical transport characteristics in WSSe under different hydrostatic environments through Raman spectroscopy, electrical conductivity, and high-resolution transmission electron microscopy (HRTEM) coupled with first-principles theoretical calculations. For nonhydrostatic conditions, WSSe endured a phase transition at 15.2 GPa, followed by a semiconductor-to-metal crossover at 25.

Efficacy and safety of sugammadex sodium in reversing rocuronium-induced neuromuscular blockade in children: An updated systematic review and meta-analysis.

Objective: In response to the differences in pharmacodynamic and pharmacokinetic characteristics of neuromuscular blocking agents between children and adults and limited studies which existing meta-analyses included, this study will update the safety and efficacy of sugammadex (Sug) sodium in reversing rocuronium-induced neuromuscular blockade in children.

Methods: Five electronic databases were searched for clinical trials on the safety and efficacy of Sug sodium in reversing rocuronium-induced neuromuscular block in children. A random-effects model was used to calculate the standardized mean difference (SMD) for primary outcomes.

Physiological response and transcriptome analyses of leguminous Walp. to drought stress.

Objective: is a shrub with high quality protein that has been widely utilized for forage grass in the semi-arid regions of China. This study aimed to enrich the currently available knowledge and clarify the detailed drought stress regulatory mechanisms in , and provide a theoretical foundation for the cultivation and resistance breeding of forage crops.

Methods: This study evaluates the response mechanism to drought stress by exploiting multiple parameters and transcriptomic analyses of a 1-year-old seedlings of in a pot experiment.

Pressure-driven structural phase transitions and metallization in the two-dimensional ferromagnetic semiconductor CrBr.

High-pressure structural, magnetic and electrical transport characteristics of CrBr were synthetically investigated using Raman scattering, electrical conductivity, high-resolution transmission electron microscopy (HRTEM) and first-principles theoretical calculations during compression and decompression under different hydrostatic conditions. Upon pressurization, CrBr underwent a second-order structural transition at 9.5 GPa, followed by the semiconductor-to-metal and magnetic switching at 25.

Attention-Based Multiscale Feature Pyramid Network for Corn Pest Detection under Wild Environment.

A serious outbreak of agricultural pests results in a great loss of corn production. Therefore, accurate and robust corn pest detection is important during the early warning, which can achieve the prevention of the damage caused by corn pests. To obtain an accurate detection of corn pests, a new method based on a convolutional neural network is introduced in this paper.

The Global Burden of Diseases attributed to high low-density lipoprotein cholesterol from 1990 to 2019.

Background: To demonstrate the real-word situation of burdens that are attributed to the risk factor of high low-density lipoprotein cholesterol (LDL-C) at the global, regional, national levels, among different age groups and between genders.

Methods: We analyzed data from the Global Burden of Disease study 2019 related to global deaths, disability-adjusted life years (DALYs), summary exposure value (SEV), average annual percentage change (AAPC), and observed to expected ratios (O/E ratios) attributable to high LDL-C from 1990 to 2019.

Results: Globally, in 2019, the total numbers of deaths and DALYs attributed to high LDL cholesterol were 1.

ASP-Det: Toward Appearance-Similar Light-Trap Agricultural Pest Detection and Recognition.

Automatic pest detection and recognition using computer vision techniques are a hot topic in modern intelligent agriculture but suffer from a serious challenge: difficulty distinguishing the targets of similar pests in 2D images. The appearance-similarity problem could be summarized into two aspects: texture similarity and scale similarity. In this paper, we re-consider the pest similarity problem and state a new task for the specific agricultural pest detection, namely ppearance imilarity est etection (ASPD) task.

The Effect of Soil Water Deficiency on Water Use Strategies and Response Mechanisms of Fisch.

We aimed to investigate the water use strategies and the responses to water shortages in Glycyrrhiza uralensis, which is a dominant species in the desert steppe. Water stress gradients included control, mild, moderate, and severe. The time intervals were 15, 30, 45, and 60 d.

Pressure-induced coupled structural-electronic transition in SnS under different hydrostatic environments up to 39.7 GPa.

A series of high-pressure Raman spectroscopy and electrical conductivity experiments have been performed to investigate the vibrational and electrical transport properties of SnS under non-hydrostatic and hydrostatic environments. Upon compression, an coupled structural-electronic transition in SnS occurred at 30.2 GPa under non-hydrostatic conditions, which was evidenced by the splitting of the E mode and the discontinuities in Raman shifts, Raman full width at half maximum (FWHM) and electrical conductivity.

Pressure-Induced Structural Phase Transition and Metallization of CrCl under Different Hydrostatic Environments up to 50.0 GPa.

High-pressure structural, vibrational, and electrical transport properties of CrCl were investigated by means of Raman spectroscopy, electrical conductivity, and high-resolution transmission electron microscopy under different hydrostatic environments using the diamond anvil cell in conjunction with the first-principles theoretical calculations up to 50.0 GPa. The isostructural phase transition of CrCl occurred at 9.

An automatic system for pest recognition and forecasting.

Background: Pests cause significant damage to agricultural crops and reduce crop yields. Use of manual methods of pest forecasting for integrated pest management is labor-intensive and time-consuming. Here, we present an automatic system for monitoring pests in large fields, with the aim of replacing manual forecasting.

Two Co(II) coordination polymers: magnetic properties and application values against chronic subdural hematoma.

In this current experiment, by applying the mixed-ligand synthesis method, two coordination polymers (CPs) containing Co(II) were created triumphantly with reaction between 1,3-bis(1-imidazoly)benzene (mbib) and Co(II) salts with the aid of diverse carboxylic ligands, and their chemical formulae are [Co(opda)(mbib)(HO)]·2HO (, Hopda is 1,2-phenylenediacetic acid) and [Co(mpda)(mbib)]·HO (, Hmpda is 1,3-phenylenediacetic acid). The two compounds' magnetic performances suggest that between the adjacent metal ions, there present the antiferromagnetic coupling. The evaluation of their treatment activity against chronic subdural hematoma was carried out and the relevant mechanism was studied simultaneously.

Optimization of location, power allocation and orientation for lighting lamps in a visible light communication system using the firefly algorithm.

To optimize the uniformity of signal-to-noise ratio (SNR) distribution in a visible light communication (VLC) system, the firefly algorithm is improved for joint optimization of location, power allocation and orientation of a light-emitting diode (LED) lamp array. Taking 16 LED lamps as an example, optimizations with a different number of degrees-of-freedom (DOF) are investigated. The orientation-involved optimizations significantly decrease average SNR and average illuminance.

Dysregulation of the miR-1275/HK2 Axis Contributes to the Progression of Hypoxia/Reoxygenation-Induced Myocardial Injury.

Objective: This research was designed to investigate the function of miR-1275 in hypoxia/reoxygenation (H/R)-induced myocardial injury and its in-depth mechanism.

Methods: Firstly, the differential expression of miR-1275 in patients with heart failure and healthy control were analyzed based on Gene Expression Omnibus (GEO) database. Then H/R model was constructed in vitro with AC16 cells.