[Clinicopathological features and prognostic factors of 216 cases with primary gastrointestinal tract non-Hodgkin's lymphoma].

Objective: To investigate the clinicopathological features of primary gastrointestinal non-Hodgkin's lymphomas (PGI-NHL) and their prognostic values.

Methods: The clinical and pathological data of 216 patients diagnosed as PGI-NHL from Zhejiang Cancer Hospital were analyzed retrospectively. χ² test, log-liner model analysis, COX proportional hazard regression analysis and Life-table survival analysis were used to analyze the survival status of the patients by SAS 8.

[Immunophenotyping and molecular genetic analysis of diffuse large B-cell lymphoma].

Objective: To perform immunophenotyping and molecular genetic analysis for diffuse large B-cell lymphoma (DLBCL), and to explore their correlation and implication for prognosis.

Methods: Immunohistochemical streptavidin peroxidase (SP) method was used to determine the expression of CD10, BCL6 and MUM1 in 59 cases of DLBCL. A Hans algorithm was used to classify DLBCL into germinal center B-cell (GCB) and non-GCB subtypes.

Structural evaluation and bioethanol production by simultaneous saccharification and fermentation with biodegraded triploid poplar.

Background: Pretreatment is a key step to decrease the recalcitrance of lignocelluloses and then increase the digestibility of cellulose in second-generation bioethanol production. In this study, wood chips from triploid poplar were biopretreated with white rot fungus Trametes velutina D10149. The effects of incubation duration on delignification efficiency and structural modification of cellulose were comparably studied, as well as the digestibility of cellulose by simultaneous saccharification and fermentation (SSF).

Improved bioconversion of poplar by synergistic treatments with white-rot fungus Trametes velutina D10149 pretreatment and alkaline fractionation.

Successive treatments with fungus and alkali were proposed to reduce the recalcitrance and improved the enzymatic digestibility of triploid poplar. Biopretreatment with Trametes velutina D10149 for 0, 4, 8, 12 and 16weeks gradually degraded hemicelluloses and lignin, and improved the digestibility of cellulose from 4.0% to 19.

Clinical, pathological, and genetic analysis of ten patients with MYH9-related disease.

MYH9-related disease (MYH9-RD) is an autosomal dominant disorder caused by mutations in the MYH9 gene. It is characterized by a triad of giant platelets, thrombocytopenia, and characteristic Döhle body-like granulocyte inclusions. In this study we report 10 unrelated patients with MYH9-RD in whom the following seven MYH9 gene mutations were found: W33R, p.

Isolation of chicken embryonic stem cell and preparation of chicken chimeric model.

Chicken embryonic stem cells (ESCs) were separated from blastoderms at stage-X and cultured in vitro. Alkaline phosphatase activity and stage-specific embryonic antigen-1 staining was conducted to detect ESCs. Then, chicken ESCs were transfected with linearized plasmid pEGFP-N1 in order to produce chimeric chicken.

An updated meta-analysis on the association of TGF-β1 gene promoter -509C/T polymorphism with colorectal cancer risk.

Aim: Published data on the association between transforming growth factor-β1 (TGF-β1) gene promoter-509C/T polymorphism and colorectal cancer (CRC) risk are inconsistent and inconclusive. To derive a more precise estimation of this association, a meta-analysis was carried out.

Methods: Meta-analysis was performed to evaluate reported studies of the relationship between TGF-β1 gene promoter-509C/T polymorphism and colorectal cancer risk using fixed-effects model and random-effects model.

Relationship of a low ankle-brachial index with all-cause mortality and cardiovascular mortality in Chinese patients with metabolic syndrome after a 6-year follow-up: a Chinese prospective cohort study.

Objective: Peripheral arterial disease (PAD) is a common clinical manifestation of the systemic atherosclerotic process, and the ankle-brachial index (ABI) is an ideal tool to diagnose PAD. Currently, there have been few long-term follow-up studies focused on the associations of the ABI with all-cause mortality and cardiovascular disease (CVD) mortality in Chinese MetS patients. The aim of this study was to evaluate the usefulness of ABI to predict the prognosis of CVD in hospitalized Chinese patients with metabolic syndrome (MetS).

Influence of bentonite particles on representative gram negative and gram positive bacterial deposition in porous media.

The significance of clay particles on the transport and deposition kinetics of bacteria in irregular quartz sand was examined by direct comparison of both breakthrough curves and retained profiles with clay particles in bacteria suspension versus those without clay particles. Two representative cell types, Gram-negative strain E. coli DH5α and Gram-positive strain Bacillus subtilis were utilized to systematically determine the influence of clay particles (bentonite) on cell transport behavior.

Biodegradation of dioxin by a newly isolated Rhodococcus sp. with the involvement of self-transmissible plasmids.

A newly isolated Rhodococcus sp. strain p52 could aerobically utilize dibenzofuran as the sole source of carbon and energy, and completely remove dibenzofuran at 500 mg l(-1) within 48 h. The strain metabolizes dibenzofuran by initial angular dioxygenation to yield 2,2',3-trihydroxybiphenyl.

Bis{1-[(1H-benzotriazol-1-yl)meth-yl]-2-methyl-1H-imidazole-κN(3)}dichlorido-cobalt(II).

In the title mononuclear complex, [CoCl(2)(C(11)H(11)N(5))(2)], the Co(II) atom is four-coordinated by two ligand N atoms and two Cl atoms in a distorted tetra-hedral geometry. In the crystal, mol-ecules are stacked through π-π inter-actions [centroid-centroid distances = 3.473 (2), 3.

[Subcellular distribution and phytotoxicity of cadmium in Alternanthera philoxeroides leaves].

A hydroponic experiment was conducted to study the subcellular distribution of Cd and mineral elements in Alternanthera philoxeroides leaves and the leaves anti-oxidative capacity and chlorophyll, soluble protein, and phytochelatins (PCs) contents under the stress of different concentration (0, 0.05, 0.1, 0.

Bis{1-[(1H-benzotriazol-1-yl)meth-yl]-2-methyl-1H-imdazole-κN(3)}dichlorido-zinc.

In the mononuclear title compound, [ZnCl(2)(C(11)H(11)N(5))(2)], the Zn(II) atom is coordinated by two Cl atoms and two imidazole N atoms in a distorted tetra-hedral geometry. Adjacent complex mol-ecules are stacked through aromatic π-π inter-actions; the closest distance between adjacent aromatic rings is 3.598 (2) Å.

Structural transformation of hemicelluloses and lignin from triploid poplar during acid-pretreatment based biorefinery process.

In order to understand the behaviors of hemicelluloses and lignin under the given acidic conditions with increasing severity, the structural characteristics were elucidated in detail by both wet chemistry methods and spectroscopic analyses in this study. Although acidic pretreatment significantly hydrolyzed the glycosidic linkages in xylan backbone and then reduced the molecular weight of xylan from 89,600 to 19,630 g/mol, a slightly increased amount of glucuronic acid was observed, probably attributing to the maintenance of ester bonds. The serious structural variation occurred in lignin macromolecule was evidenced by the extensive degradation of β-O-4 ether linkages and resinol substructure, together with the changes in the ratios of the three monolignols in ester-bond, ether-bond and non-condensed phenolic compounds.

Expression and prognostic value of ING3 in human primary hepatocellular carcinoma.

The tumor-suppressor ING3 has been shown to be involved in tumor transcriptional regulation, apoptosis and the cell cycle. Some studies have demonstrated that ING3 is dysregulated in several types of cancers. However, the expression and function of ING3 in human hepatocellular carcinoma (HCC) remains unclear.

[Role of pharmacokinetic monitoring of serum fluorouracil concentration in patients with local advanced and metastatic colorectal cancer and further improving efficacy of fluorouracil-based chemotherapy].

Objective: To investigate the relationship between serum concentration of fluorouracil and therapeutic efficacy as well as adverse reactions in patients with unresectable locally advanced or measurable metastatic colorectal cancer, and to analyze its role in further improving therapeutic efficacy and reducing adverse reactions of fluorouracil-based chemotherapy.

Methods: Eighty-six patients were randomly assigned into three groups according to the average plasma concentration of fluorouracil after three cycles of chemotherapy with the initial regimen of two weeks FOLFOX-4 (oxaliplatin + leucovorin + fluorouracil) or FOLFIRI (irinotecan + leucovorin + fluorouracil): group 1 (plasma concentration of fluorouracil < 25 ng/ml), group 2 (25 - 35 ng/ml) and group 3 (> 35 ng/ml). The blood samples were taken at 12 h after continuous infusion of fluorouracil in each cycle and the plasma concentration of fluorouracil was detected by high performance liquid chromatography (HPLC) (about 5 am ± 1 h).

Rituximab in treatment of primary gastric diffuse large B-cell lymphoma.

In the rituximab era, the optimal treatment modality for primary gastric diffuse large B-cell lymphoma (PG-DLBCL) still remains unclear. We performed a retrospective, multicenter analysis of 65 patients with PG-DLBCL to assess the efficacy and toxicity of the addition of rituximab to conventional chemotherapy. When compared with conventional chemotherapy, there was a trend that rituximab plus chemotherapy yielded a higher complete response rate, 5-year event-free survival (EFS) rate and 5-year overall survival (OS) rate, but this was not statistically significant.

Deposition kinetics of MS2 bacteriophages on clay mineral surfaces.

The deposition of bacteriophage MS2 on bare and clay-coated silica surfaces was examined in both monovalent (NaCl) and divalent (CaCl(2) and MgCl(2)) solutions under a wide range of environmentally relevant ionic strength and pH conditions by utilizing a quartz crystal microbalance with dissipation (QCM-D). Two types of clay, bentonite and kaolinite, were concerned in this study. To better understand MS2 deposition mechanisms, QCM-D data were complemented by zeta potentials measurements and Derjaguin-Landau-Verwey-Overbeek (DLVO) interaction forces calculation.

Influence of humic acid on the transport behavior of bacteria in quartz sand.

The significance of natural organic matter (NOM) on the transport of bacteria in packed porous media (quartz sand) was examined in both NaCl and CaCl(2)-NaCl mixing solutions at pH 6.0. Three representative cell types (with EPS), Rhodococcus sp.

[Expression, purification and characterization of non-specific Serratia nuclease in Escherichia coli].

To efficiently produce non-specific nuclease (NU) of Serratia marcescens through recombinant overexpression approach and to characterize the purified NU. The nuclease gene was amplified from the genomic DNA of Serratia marcescens by PCR and fused into vector pMAL-c4X with maltose binding protein (MBP) tag. The recombinant vector verified by DNA sequencing was transformed into Escherichia coli BL21.

E-cadherin (CDH1) gene promoter polymorphism and the risk of colorectal cancer : a meta-analysis.

Objective: Published data on the relationship between E-cadherin (CDH1) gene promoter polymorphisms and colorectal cancer (CRC) risk are inconclusive. To derive a more precise estimation of the relationship, a meta-analysis was carried out.

Methods: In this meta-analysis, we evaluated reported studies of associations between polymorphisms of CDH1 gene promoter -160 C > A and -347 G > GA site, and CRC risk using fixed-effects model and random-effects model.

Antimicrobial properties of MX-2401, an expanded-spectrum lipopeptide active in the presence of lung surfactant.

MX-2401 is an expanded-spectrum lipopeptide antibiotic selective for Gram-positive bacteria that is a semisynthetic analog of the naturally occurring lipopeptide amphomycin. It was active against Enterococcus spp., including vancomycin-sensitive Enterococcus (VSE), vanA-, vanB-, and vanC-positive vancomycin-resistant Enterococcus (VRE), linezolid- and quinupristin-dalfopristin-resistant isolates (MIC(90) of 4 μg/ml), methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive S.

Production of xylooligosaccharides by xylanase from Pichia stipitis based on xylan preparation from triploid Populas tomentosa.

Xylooligosaccharides (XOS) with DP 2-4 are important synbiotics used as food ingredients based on its prebiotic characteristics. In this work, the production of XOS from lignocellulosic material was performed by combined chemical-enzymatic methods. Xylan was prepared from triploid Populas tomentosa, and bioconverted into XOS by crude xylanase solution obtained from Pichia stipitis.

Assessing the visual quality of green landscaping in rural residential areas: the case of Changzhou, China.

Changzhou is a typical waterside municipality with approximately 2,500 years of history located south of the Yangtze River. It was an agriculturally oriented region but is becoming an industrialized region. Rural green landscaping in this region possesses characteristics of traditional Chinese gardens.