LINC02987 suppression hepatocellular carcinoma progression by modulating autophagy via the miR-338-3p/ATG12 axis.

Hepatocellular carcinoma (HCC), the most common primary liver cancer, is marked by a high mortality rate, with the misregulation of long non-coding RNAs (LncRNAs) playing a key role in its development. Here, we studied the role of LINC02987 in HCC. We employed bioinformatics tools to identify LncRNAs and miRNAs that exhibit differential expression in HCC.

A quinoline colorimetric ionic liquid probe by electrostatic enhancement for visual detection of Fe in food.

Excessive or insufficient iron(Ⅲ) will pose burdens of human body, and its content is the key to control the function of iron-fortified food. In this regard, a functionalized quinoline ionic probe, benefiting from the electrostatic attraction, was designed for the colorimetric detection of Fe in food. This probe formed a 2: 1 complex with Fe, altering the UV-vis spectra and solution color.

Reductive soil disinfestation influences microbial aging of low-density polyethylene and polyhydroxyalkanoate microplastics and microbial communities in plastispheres.

The extensive use of plastic products has led to the accumulation of microplastics (MPs) in agricultural soils, raising concerns about their fate in various environments. Reductive soil disinfestation (RSD) treatment is increasingly being adopted in various countries to address agricultural soil health issues. However, the treatment can alter the soil microbial environment, potentially affecting the fate of contaminants, including MPs.

Rapid qualitative and quantitative detection for adulteration of Atractylodis Rhizoma using hyperspectral imaging combined with chemometric methods.

In the field of traditional Chinese medicine, Atractylodis Rhizoma (AR) is commonly used for various diseases due to its excellent ability to dry dampness and strengthen the spleen, especially popular in East Asia. The aim of this study is to proposed Hyperspectral Imaging (HSI) in combination with chemometric methods for the rapid qualitative and quantitative detection of AR adulteration with other types of powder. Partial Least Squares Discriminant Analysis (PLS-DA) was used to construct the classification models the best, with the First-order Derivative (F-D) preprocessing method.

Clinical efficacy and mechanism of the combination of autologous platelet-rich gel and recombinant human acidic fibroblast growth factor in the management of refractory diabetic foot.

Objective: This study aims to explore the influence of combining autologous platelet-rich gel (APG) with continuous vacuum-sealed drainage (CVSD) and the exogenous recombinant human acidic fibroblast growth factor (rh-aFGF) on the healing processes of diabetic foot ulcers (DFU). The primary objective is to elucidate the complex molecular mechanisms associated with DFU, providing innovative perspectives for its treatment.

Methods: Ninety patients diagnosed with DFU were randomly allocated into three distinct groups.

Microplastics in indoor and outdoor environments in China: Characteristic and human exposure risk assessment.

Human exposure to microplastics (MPs) has led to global health concerns, but our knowledge of the characteristics and human exposure of airborne MPs is limited. Adults may have different exposure patterns and lifestyles from other age groups. Therefore, this study aims to determine the MPs exposure among adults at various locations and during different time periods.

Exploring the barriers to the development of organizational health literacy in health institutions to meet the needs of older patients from multiple perspectives: a mixed-methods study.

Background: The latest evidence on health literacy in China revealed that only 8% of individuals aged 65-69 years, a segment of the geriatric population, possess adequate health literacy levels, indicating a low level in China. Studies have demonstrated that improving the organizational health literacy of healthcare institutions is a crucial approach to addressing low health literacy among older patients.

Objective: To explore the obstacles hindering the advancement of organizational health literacy in healthcare institutions in meeting the needs of older patients.

Ruthenium-Catalyzed Synthesis of 2-Pyrazolines via Acceptorless Dehydrogenative Coupling of Allylic Alcohols with Hydrazines.

Described herein is the synthesis of 2-pyrazolines via acceptorless dehydrogenative coupling of allylic alcohols with hydrazines based on a Ru(CO)/NHC-phosphine-phosphine ligand catalytic system. The reaction not only exhibits low catalyst loading (only 0.3 mol %), wide substrate scope, good to excellent yields, and high selectivity but also omits the use of sacrificial hydrogen acceptor with only H and HO as byproducts, making the reaction green and atom-economical.

Efficient degradation of carbamazepine and metagenomic investigations of anodic biofilm in microbial fuel cells.

Environmental contamination with carbamazepine is a considerable global problem. In this study, two-compartment microbial fuel cells (MFCs) were constructed to investigate the degradation performance of carbamazepine, and the degradation mechanism was further explored by using metagenomic analysis. The results showed that MFCs exhibited excellent carbamazepine removal performance and also generated electricity.

A new Bowman-Birk type protease inhibitor regulated by MeJA pathway in maize exhibits anti-feedant activity against the Ostrinia furnacalis.

Jasmonic acid (JA), an important plant hormone, plays a crucial role in defending against herbivorous insects. In this study, we have identified a new Bowman-Birk type protease inhibitor (BBTI) protein in maize that is regulated by the JA pathway and exhibits significant antifeedant activity, which is notably induced by exogenous Methyl Jasmonate and Ostrinia furnacalis feeding treatments. Bioinformatics analysis revealed significant differences in the BBTI protein among different maize inbred lines, except for the conserved domain.

A highly sensitive fluorescent probe based on functionalised ionic liquids for timely detection of trace Hg and CHHg in food.

A novel fluorescent probe was fabricated using fluorescein-based ionic liquids (ILs) to effectively achieve rapid and accurate detection of Hg and CHHg in food. A probe developed by addition of modified fluorescein into the functionalised ILs presented a promising sensitivity toward Hg and CHHg at concentrations of 0.4 and 60 nM, respectively.

Exploring the glycoprotein washing fluid-assisted cleanup for the restoration of oil-contaminated shorelines with environmental integrity.

Spilled oil in ocean can spread to the shoreline and cause long-term impacts on the shoreline's ecological environment. Therefore, removing oil accumulated on shorelines is crucial. This study proposed an innovative ovalbumin (OVA) fluid-assisted method for the cleanup of oiled shoreline substrates.

Revealing the key antioxidant compounds and potential action mechanisms of Chinese Cabernet Sauvignon red wines by integrating UHPLC-QTOF-MS-based untargeted metabolomics, network pharmacology and molecular docking approaches.

In recent years, red wine drinking has become more popular in China owing to its antioxidant effects. However, the key antioxidant compounds and their action mechanisms of Chinese red wines are still unclear. Herein, the antioxidant activities and chemical compositions of 45 Chinese Cabernet Sauvignon red wine samples were determined using chemical antioxidant assays and an UHPLC-QTOF-MS-based untargeted metabolomics method.

Resveratrol suppresses liver cancer progression by downregulating AKR1C3: targeting HCC with HSA nanomaterial as a carrier to enhance therapeutic efficacy.

The enzyme AKR1C3 plays a crucial role in hormone and drug metabolism and is associated with abnormal expression in liver cancer, leading to tumor progression and poor prognosis. Nanoparticles modified with HSA can modulate the tumor microenvironment by enhancing photodynamic therapy to induce apoptosis in tumor cells and alleviate hypoxia. Therefore, exploring the potential regulatory mechanisms of resveratrol on AKR1C3 through the construction of HSA-RSV NPs carriers holds significant theoretical and clinical implications for the treatment of liver cancer.

AAV gene replacement therapy for treating MPS IIIC: Facilitating bystander effects via EV-mRNA cargo.

MPS IIIC is a lysosomal storage disease caused by mutations in heparan-α-glucosaminide N-acetyltransferase (HGSNAT), for which no treatment is available. Because HGSNAT is a trans-lysosomal-membrane protein, gene therapy for MPS IIIC needs to transduce as many cells as possible for maximal benefits. All cells continuously release extracellular vesicles (EVs) and communicate by exchanging biomolecules via EV trafficking.

Highly selective and additive-free Pd(OAc)/CPP catalyzed hydroaminocarbonylation of alkynes.

Herein, the synthesis of branched α,β-unsaturated amides by a hydroaminocarbonylation reaction of alkynes with various amine substrates such as aromatic amines, aliphatic amines, solid amine sources like NHHCO, and even strongly basic piperidines is reported, using a Pd(OAc)/hybrid N-heterocyclic carbene-phosphine-phosphine (CPP) catalytic system. The reactions feature no additives, wide substrate scope, high selectivity (b/l > 99 : 1) and excellent yields. Mechanistic studies have disclosed that the reaction takes place a palladium hydride pathway.

mA RNA methylation drives kidney fibrosis by upregulating β-catenin signaling.

N-methyladenosine (mA) methylation plays a crucial role in various biological processes and the pathogenesis of human diseases. However, its role and mechanism in kidney fibrosis remain elusive. In this study, we show that the overall level of mA methylated RNA was upregulated and the mA methyltransferase METTL3 was induced in kidney tubular epithelial cells in mouse models and human kidney biopsies of chronic kidney disease (CKD).

AntDAS-GCMS: A New Comprehensive Data Analysis Platform for GC-MS-Based Untargeted Metabolomics with the Advantage of Addressing the Time Shift Problem.

Over the years, a number of state-of-the-art data analysis tools have been developed to provide a comprehensive analysis of data collected from gas chromatography-mass spectrometry (GC-MS). Unfortunately, the time shift problem remains unsolved in these tools. Here, we developed a novel comprehensive data analysis strategy for GC-MS-based untargeted metabolomics (AntDAS-GCMS) to perform total ion chromatogram peak detection, peak resolution, time shift correction, component registration, statistical analysis, and compound identification.

Esters-targeted colorimetric sensor array for the authenticity discrimination of strong-aroma baijiu with different origins.

Baijiu authenticity has been a frequent problem driven by economic interests in recent years, so it is important to discriminate against baijiu with different origins. Herein, we proposed a simple and efficient esters-targeted colorimetric sensor array mediated by hydroxylamine hydrochloride. Esters undergo a nucleophilic addition reaction with hydroxylamine hydrochloride to form hydroxamic acid, which rapidly forms a purplish red ferric hydroxamate under FeCl·6HO.