[Molecular identification and efficacy analysis of herbs at Orussey Herbal Market, Phnom Penh, Cambodia].

Cambodia is rich in medicinal plant resources. One hundred and thirty-three medicinal material samples, including the hole herb, root, stem/branch, leaf, flower, fruit, seed, and resin, were collected from the Orussey Herbal Market in Phnom Penh, Cambodia, and then authenticated by ITS and psbA-trnH. A total of 46 samples were identified based on ITS sequences, belonging to 24 families, 40 genera, and 42 species.

UTMD-mediated delivery of miR-21-5p inhibitor suppresses the development of lung cancer.

Background: Ultrasound-targeted microbubble destruction (UTMD) is a new type of gene delivery technology. MiR-21-5p was highly expressed in a variety of cancers. In this paper, miR-21-5p inhibitor was transfected into lung cancer cells by UTMD to observe its role in lung cancer.

Robust Adaptive Self-Structuring Neural Network Bounded Target Tracking Control of Underactuated Surface Vessels.

This paper studies the target-tracking problem of underactuated surface vessels with model uncertainties and external unknown disturbances. A composite robust adaptive self-structuring neural-network-bounded controller is proposed to improve system performance and avoid input saturation. An extended state observer is proposed to estimate the uncertain nonlinear term, including the unknown velocity of the tracking target, when only the measurement values of the line-of-sight range and angle can be obtained.

Lithium Storage Mechanism and Application of Micron-Sized Lattice-Reversible Binary Intermetallic Compounds as High-Performance Flexible Lithium-Ion Battery Anodes.

A strategy of lattice-reversible binary intermetallic compounds of metallic elements is proposed for applications in flexible lithium-ion battery (LIB) anode with high capacity and cycling stability. First, the use of metallic elements can ensure excellent electronic conductivity and high capacity of the active anode substance. Second, binary intermetallic compounds possess a larger initial lattice volume than metallic monomers, so that the problem of volume expansion can be alleviated.

Factors associated with brain white matter damage in type 2 diabetes mellitus: a tract-based spatial statistics study.

Background: The pathogenesis and related factors of central nervous system abnormality in patients with type 2 diabetes mellitus (T2DM) have always been the focus of clinical research.

Purpose: To compare and analyze the area of white matter (WM) damage in patients with T2DM based on their level of hemoglobin A (HBA) and discuss any related factors.

Material And Methods: Based on their levels of HBA, 87 patients with T2DM were divided into three groups (Group B, C, or D), of which 29 non-diabetic volunteers served as the control group (Group A).

Conductive bimetal organic framework nanorods decorated with highly dispersed CoOnanoparticles as bi-functional electrocatalyst.

The poor electronic conductivity and low intrinsic electrocatalytic activity of metal organic frameworks (MOFs) greatly limit their direct application in electrocatalytic reactions. Herein, we report a conductive two-dimensional-conjugated Ni and Co bimetal organic framework (MOF)-NiCo-(2,3,6,7,10,11-hexaiminotriphenylene) (NiCo-HITP) nanorods decorated with highly dispersed CoOnanoparticles (NPs) as a promising bi-functional electrocatalyst towards oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) through an effective and facile strategy by modifying the rod-shaped -NiHITPcrystals using cobalt ions. The triggered electrocatalytic activity of the resulting MOF-based materials was achieved by increasing the electrical conductivity (7.

Choose Appropriate Subproblems for Collaborative Modeling in Expensive Multiobjective Optimization.

In dealing with the expensive multiobjective optimization problem, some algorithms convert it into a number of single-objective subproblems for optimization. At each iteration, these algorithms conduct surrogate-assisted optimization on one or multiple subproblems. However, these subproblems may be unnecessary or resolved.

Urinary donor-derived cell-free DNA as a non-invasive biomarker for BK polyomavirus-associated nephropathy.

BK polyomavirus-associated nephropathy (BKPyVAN) is a common cause of allograft failure. However, differentiation between BKPyVAN and type I T cell-mediated rejection (TCMR) is challenging when simian virus 40 (SV40) staining is negative, because of the similarities in histopathology. This study investigated whether donor-derived cell-free DNA (ddcfDNA) can be used to differentiate BKPyVAN.

A Novel Method of Mouse RPE Explant Culture and Effective Introduction of Transgenes Using Adenoviral Transduction for Studies in AMD.

Degeneration of retinal pigment epithelium (RPE) is one of the most critical phenotypic changes of age-related macular degeneration (AMD), the leading cause of vision loss in the elderly. While cultured polarized RPE cells with original properties are valuable in models to study RPE biology and the consequences of genetic and/or pharmacological manipulations, the procedure to establish mouse primary PRE cell culture or pluripotent stem cell-derived RPE cells is time-consuming and yields a limited number of cells. Thus, establishing a mouse RPE culture system is highly desirable.

Exogenous ascorbic acid application alleviates cadmium toxicity in seedlings of two wheat (Triticum aestivum L.) varieties by reducing cadmium uptake and enhancing antioxidative capacity.

The aggravation of soil cadmium (Cd) pollution is a serious threat to human food health and safety. To reduce Cd uptake and alleviate Cd toxicity in staple food of wheat, a completely random experiment was performed to investigate the effect of exogenous ascorbic acid (AsA) on Cd toxicity in two wheat varieties (L979 and H27). In this study, the treatments with combinations of Cd (0, 5, and 10 µmol L) and AsA (0, 50, and 200 µmol L) were applied in a hydroponic system.

Linguistic emergence from a networks approach: The case of modern Chinese two-character words.

The models of linguistic networks and their analytical tools constitute a potential methodology for investigating the formation of structural patterns in actual language use. Research with this methodology has just started, which can hopefully shed light on the emergent nature of linguistic structure. This study attempts to employ linguistic networks to investigate the formation of modern Chinese two-character words (as structural units based on the chunking of their component characters) in the actual use of modern Chinese, which manifests itself as continuous streams of Chinese characters.

Development of recombinant COVID-19 vaccine based on CHO-produced, prefusion spike trimer and alum/CpG adjuvants.

COVID-19 pandemic has severely impacted the public health and social economy worldwide. A safe, effective, and affordable vaccine against SARS-CoV-2 infections/diseases is urgently needed. We have been developing a recombinant vaccine based on a prefusion-stabilized spike trimer of SARS-CoV-2 and formulated with aluminium hydroxide and CpG 7909.

[Identification and characterization of DIR gene family in Schisandra chinensis].

Dirigent(DIR) proteins are involved in the biosynthesis of lignin, lignans, and gossypol in plants and respond to biotic and abiotic stresses. Based on the full-length transcriptome of Schisandra chinensis, bioinformatics methods were used to preliminarily identify the DIR gene family and analyze the physico-chemical properties, subcellular localization, conserved motifs, phylogeny, and expression patterns of the proteins. The results showed that a total of 34 DIR genes were screened and the encoded proteins were 156-387 aa.

Tumor Immune Microenvironment Landscape in Glioma Identifies a Prognostic and Immunotherapeutic Signature.

The tumor immune microenvironment (TIME) has been recognized to be associated with sensitivity to immunotherapy and patient prognosis. Recent research demonstrates that assessing the TIME patterns on large-scale samples will expand insights into TIME and will provide guidance to formulate immunotherapy strategies for tumors. However, until now, thorough research has not yet been reported on the immune infiltration landscape of glioma.

Neutrophil-Derived Proteases Contribute to the Pathogenesis of Early Diabetic Retinopathy.

Purpose: Previous studies indicate that leukocytes, notably neutrophils, play a causal role in the capillary degeneration observed in diabetic retinopathy (DR), however, the mechanism by which they cause such degeneration is unknown. Neutrophil elastase (NE) is a protease released by neutrophils which participates in a variety of inflammatory diseases. In the present work, we investigated the potential involvement of NE in the development of early DR.

Regulation of Hepatitis B Virus Virion Release and Envelopment Timing by Nucleocapsid and Envelope Interactions.

Interactions between the N-terminal (assembly) domain (NTD), the linker region of the hepatitis B virus (HBV) capsid protein, and the large (L) envelope protein are required for virion formation, which occurs via budding of cytoplasmic mature nucleocapsids (NCs) containing the relaxed circular (RC) DNA genome into an intracellular membrane compartment containing viral envelope proteins. L-capsid interactions also negatively regulate covalently closed circular (CCC) DNA formation, which occurs after RC DNA release from mature NCs and nuclear import. We have now found that L could increase RC DNA in cytoplasmic mature NCs that are destabilized due to mutations in the NTD or the linker, even in those that apparently fail to support secretion of complete virions extracellularly.

Single sheets of graphene for fabrication of fibers with enhanced mechanical properties.

This paper reports the fabrication and mechanical properties of macroscale graphene fibers (diameters of 10 to 100 μm with lengths upwards of 2 cm) prepared from a single sheet of single-layer graphene grown chemical vapor deposition (CVD). The breaking strength of these graphene fibers increased with consecutive tensile test measurements on a single fiber, where fiber fragments produced from a prior test exhibited larger breaking strengths. Additionally, we observed an overall reduction of surface folds and wrinkles, and an increase in their alignment parallel to the tensile direction.

Astrocytic Hydrogen Sulfide Regulates Supraoptic Cellular Activity in the Adaptive Response of Lactating Rats to Chronic Social Stress.

Maternal social stress among breastfeeding women can be adapted in chronic process. However, neuroendocrine mechanisms underlying such adaptation remain to be identified. Here, we report the effects of 2 hr/day unfamiliar male rat invasion (UMI) stress on maternal behaviors in lactating rats during postpartum day 8 (UMI8) to postpartum day 12 (UMI12).

Multifocal Visual Evoked Potentials (mfVEP) for the Detection of Visual Field Defects in Glaucoma: Systematic Review and Meta-Analysis.

Some discrepancies have been observed in the diagnostic efficacy of multifocal visual evoked potential (mfVEP) when evaluating visual field defects in glaucoma patients. Therefore, we evaluated the diagnostic precision of the mfVEP in glaucoma to find its best diagnostic indicator. A systematic review and meta-analysis of quantitative studies published up to 1 April 2021 was performed.

The semaphorin 4A-neuropilin 1 axis alleviates kidney ischemia reperfusion injury by promoting the stability and function of regulatory T cells.

Previous studies have suggested the role of CD4Foxp3 regulatory T cells (Tregs) in protection against kidney ischemia reperfusion injury via their immunosuppressive properties. Unfortunately, the associated mechanisms of Tregs in kidney ischemia reperfusion injury have not been fully elucidated. Semaphorin 4A (Sema4A) is essential for maintaining the immunosuppressive capacity of Tregs in tumors.

Efficient method for the calculation of the optical force of multiple nanoparticles based on the coupling theory of quasinormal modes.

An efficient method is proposed for the calculation of the optical force of multiple nanoparticles. In this method, the optical force is calculated by integrating the Maxwell stress tensor (MST) over a closed surface encompassing the nanoparticle. The electromagnetic (EM) field required for evaluating the MST is computed with the coupling theory of quasinormal modes (QNMs), in which the EM field is expanded onto a small set of QNMs of each nanoparticle.

Analysis of the Expression and Prognostic Value of Annexin Family Proteins in Bladder Cancer.

Background: Bladder cancer (BC) is the most common tumor of the urinary system. Non-muscle-invasive bladder cancer (NMIBC) has a high recurrence rate after surgery, and patients with muscle-invasive bladder cancer (MIBC) have poor quality of life after radical surgery. Understanding the molecular mechanism of bladder cancer is helpful for providing a more appropriate treatment approach.