The low enoyl-acyl carrier protein reductase activity of FabI2 is responsible for the high unsaturated fatty acid composition in Sinorhizobium meliloti.

Background: Sinorhizobium meliloti is noted for its exceptional capacity to produce unsaturated fatty acids (UFAs). Earlier studies have indicated that S. meliloti primarily employs the FabA-FabB pathway for UFA synthesis, however, the mechanisms remain elusive.

A Nonessential Sfp-Type Phosphopantetheinyl Transferase Contributes Significantly to the Pathogenicity of .

4'-Phosphopantetheinyl transferases (PPTases) play important roles in the posttranslational modifications of bacterial carrier proteins, which are involved in various metabolic pathways. Here, we found that and encoded a functional AcpS-type and Sfp-type PPTase, respectively, in GMI1000, and both are capable of modifying AcpP1, AcpP2, AcpP3, and AcpP5 proteins. is located on the megaplasmid, which does not affect strain growth and fatty acid synthesis but significantly contributes to the virulence of and preferentially participates in secondary metabolism.

ATF3 mediates PM2.5-induced apoptosis and inflammation in ovarian granulosa cells.

Particulate matter 2.5 (PM2.5) pollution has emerged as a major global public health concern because of its adverse effects on human health.

Betulonic Acid Inhibits Type-2 Porcine Reproductive and Respiratory Syndrome Virus Replication by Downregulating Cellular ATP Production.

Porcine reproductive and respiratory syndrome (PRRS), caused by PRRS virus (PRRSV) infection, has been a serious threat to the pork industry worldwide and continues to bring significant economic loss. Current vaccination strategies offer limited protection against PRRSV transmission, highlighting the urgent need for novel antiviral approaches. In the present study, we reported for the first time that betulonic acid (BA), a widely available pentacyclic triterpenoids throughout the plant kingdom, exhibited potent inhibition on PRRSV infections in both Marc-145 cells and primary porcine alveolar macrophages (PAMs), with IC values ranging from 3.

The puzzle of two tandem acyl-CoA ligases of F1.

Unlabelled: The F1 genome and those of many other pseudomonads contain two tandem genes encoding acyl-CoA ligases Pput_1340 () and Pput_1339 () with Pput_1339 () being the upstream gene. The designation was assigned when both genes were found to complement the growth of an acyl-CoA synthetase deletion strain with oleic acid as sole carbon source. Site-directed mutagenesis showed that residues of the ATP/AMP domain required for function of FadD were also essential for full function of FadD1 and FadD2.

Realizing Ultrahigh Cycle Life Anode for Sodium-Ion Batteries through Heterostructure Design and Introducing Electro Active Polymer Coating.

BiS has attracted increasing attention in sodium-ion batteries (SIBs) for its high theoretical capacity and low discharge platform. However, the sodium storage performance of BiS is limited by poor electrical conductivity and volume expansion during cycling. Herein, we report a special polypyrrole (PPy)-coated MoS/BiS (MBS@PPy) heterostructure composite obtained by hydrothermal reaction as an anode material for SIB.

Assessing the efficiency of UAV for pesticide application in disease management of peanut crop.

Background: Effective utilization of plant protection UAVs in peanut cultivation management necessitates a comprehensive grasp of how application volume rates and pesticides influence peanut leaf spot and rust control. This study aimed to compare the effects of application volume rates and pesticides on droplet deposition, disease, leaf retention rate and peanut yield. A T20 plant protection unmanned aerial vehicle (UAV) sprayer was used to apply four various pesticide doses.

SUMOylation of zebrafish transcription factor Zbtb21 affects its transcription activity.

Background: Post-translational modification by Small Ubiquitin-like MOdifier (SUMO) is an important mechanism to regulate protein activity, protein stability, and localization of substrates. Zbtb21 is a zinc finger and BTB (Broad-complex, Tram-track and Bric à brac) domain-containing transcription factor. Bioinformatic prediction suggests several putative SUMOylated sites in Zbtb21 protein.

Can rectal MRI and endorectal ultrasound accurately predict the complete response to neoadjuvant immunotherapy for rectal cancer?

Background: Standardized assessments of clinical complete response (cCR) to neoadjuvant chemoradiotherapy (nCRT) for rectal cancer have been established, but their utility and accuracy remain unclear. This study aimed to evaluate the clinical diagnostic value of rectal magnetic resonance imaging (MRI) and endorectal ultrasonography (ERUS) for the determination of cCRs after neoadjuvant immunotherapy and to investigate the concordance between cCR and pathological complete response (pCR).

Methods: Ninety-four patients with rectal cancer treated with neoadjuvant radiotherapy with or without immunotherapy were included.

Intervention Effect of Mindfulness-Based Mental Health Education Therapy on Adolescents Mobile Phone Addiction and Cognitive Tendency.

Background: Teenagers are more prone to mobile phone addiction than other age groups due to their lack of self-control. This addiction to mobile phones severely affects their physical and mental health, causing irreversible harm as they grow older. As such, exploring suitable psychotherapy for their physical and mental development needs is essential, in ways that effectively change teenagers' mobile phone addictive behaviors.

An atypical 3-ketoacyl ACP synthase III required for acyl homoserine lactone synthesis in pv. B728a.

The last step of the initiation phase of fatty acid biosynthesis in most bacteria is catalyzed by the 3-ketoacyl-acyl carrier protein (ACP) synthase III (FabH). pv. strain B728a encodes two FabH homologs, Psyr_3467 and Psyr_3830, which we designated PssFabH1 and PssFabH2, respectively.

Erratum: [Corrigendum] Changes in expression of p53 and inflammatory factors in patients with ulcerative colitis.

[This corrects the article DOI: 10.3892/etm.2019.

Diversity in fatty acid elongation enzymes: The FabB long-chain β-ketoacyl-ACP synthase I initiates fatty acid synthesis in Pseudomonas putida F1.

The condensation of acetyl-CoA with malonyl-acyl carrier protein (ACP) by β-ketoacyl-ACP synthase III (KAS III, FabH) and decarboxylation of malonyl-ACP by malonyl-ACP decarboxylase are the two pathways that initiate bacterial fatty acid synthesis (FAS) in Escherichia coli. In addition to these two routes, we report that Pseudomonas putida F1 β-ketoacyl-ACP synthase I (FabB), in addition to playing a key role in fatty acid elongation, also initiates FAS in vivo. We report that although two P.

Znf687 recruits Brd4-Smrt complex to regulate gfi1aa during neutrophil development.

Neutrophils are key component of the innate immune system in vertebrates. Diverse transcription factors and cofactors act in a well-coordinated manner to ensure proper neutrophil development. Dysregulation of the transcriptional program triggering neutrophil differentiation is associated with various human hematologic disorders such as neutropenia, neutrophilia, and leukemia.

Efficacy and safety of radiotherapy combined with anti-angiogenic therapy and immune checkpoint inhibitors in MSS/pMMR metastatic colorectal cancer.

Purpose: Several studies have demonstrated the effectiveness of anti-angiogenic drugs in combination with immune checkpoint inhibitors (ICIs) in patients with microsatellite stable (MSS) or mismatch repair proficient (pMMR) metastatic colorectal cancer (mCRC). However, whether combination radiotherapy (RT) can further improve the prognosis of mCRC patients after second-line treatment remains to be explored.

Methods: Retrospective analysis of data from mCRC patients who received anti-angiogenic targeted therapy (TT) and immunotherapy (IT) with or without RT after the failure of standard therapy.

Comparative genomics analysis of strains from a community.

Background: is a multidrug-resistant (MDR) opportunistic pathogen with high resistance to most clinically used antimicrobials. The dissemination of MDR and difficult treatment of its infection in clinical settings are global issues.

Methods: To provide more genetic information on and find a better treatment strategy, we isolated five , SMYN41-SMYN45, from a Chinese community that were subjected to antibiotic susceptibility testing, biofilm formation assay, and whole-genome sequencing.

Effects of Age on Compounds, Metabolites and Meat Quality in Beijing-You Chicken Breast Meat.

The physical properties, free amino acids, and metabolites of Beijing-You chicken (BYC) breast meat aged 90, 120, and 150 days were analyzed to investigate the flavor changes with age. The shear force and intramuscular fat increased from 90 to 120 days significantly. The contents of total free amino acids and essential amino acids decreased from 90 to 120 days significantly.

Circulating cell-free DNA fragmentation is a stepwise and conserved process linked to apoptosis.

Background: Circulating cell-free DNA (cfDNA) is a pool of short DNA fragments mainly released from apoptotic hematopoietic cells. Nevertheless, the precise physiological process governing the DNA fragmentation and molecular profile of cfDNA remains obscure. To dissect the DNA fragmentation process, we use a human leukemia cell line HL60 undergoing apoptosis to analyze the size distribution of DNA fragments by shallow whole-genome sequencing (sWGS).

Resistance risk and resistance-related point mutations in cytochrome b of florylpicoxamid in Colletotrichum scovillei.

Anthracnose caused by Colletotrichum scovillei is one of the most destructive diseases of chili worldwide. Florylpicoxamid is a new quinone inside inhibitor (QiI) fungicide, which shows intensively inhibitory activity against C. scovillei.

Pathway Genes in Ovarian Cancer: Clinical Significance and Associations with Immune Cell Infiltration.

Background: Activation of the signaling pathway is associated with tumorigenesis. The aim of this study was to investigate pathway gene functions and regulatory mechanisms in ovarian cancer (OC).

Methods: We conducted a bioinformatics analysis of publicly available datasets in order to identify potential -related mechanisms, associated genes, biological pathways, and their relation to immune function.

Comprehensive analysis of TLX2 in pan cancer as a prognostic and immunologic biomarker and validation in ovarian cancer.

T cell leukemia homeobox 2 (TLX2) plays an important role in some tumors. Bioinformatics and experimental validation represent a useful way to explore the mechanisms and functions of TLX2 gene in the cancer disease process from a pan cancer perspective. TLX2 was aberrantly expressed in pan cancer and cell lines and correlated with clinical stage.

Proteomic Profiling of Thigh Meat at Different Ages of Chicken for Meat Quality and Development.

Chicken age contributes to the meat characteristics; however, knowledge regarding the pathways and proteins associated with meat quality and muscle development are still scarce, especially in chicken thigh meat. Hence, the objective of this study was to elucidate the intricate relationship between these traits by liquid chromatography mass spectrometry at three different ages. A total of 341 differential expressed proteins (DEPs) were screened out (fold change ≥ 1.

A novel strategy to control Pseudomonas syringae through inhibition of type III secretion system.

Pseudomonas syringae (P. syringae) is a highly prevalent Gram-negative pathogen with over 60 pathogenic variants that cause yield losses of up to 80% in various crops. Traditional control methods mainly involve the application of antibiotics to inactivate pathogenic bacteria, but large-scale application of antibiotics has led to the development of bacterial resistance.