Depletion of NK cells attenuates paraquat-induced acute lung injury by manipulating macrophage polarization.

Acute lung injury is the main causative factor in paraquat dichloride (PQ)-induced mortality. The innate immune system-triggered detrimental inflammatory cascade plays a vital role in PQ-induced acute lung injury. However, the role of natural killer (NK) cells, which are essential for innate response, in PQ-induced acute lung injury remains largely unknown.

Generation of Hematopoietic-Like Stem Cells from Adult Human Peripheral Blood Following Treatment with Platelet-Derived Mitochondria.

Adult stem cells represent a potential source for cellular therapy to treat serious human diseases. We characterized the insulin-producing cells from adult peripheral blood (designated PB-IPC), which displayed a unique phenotype. Mitochondria are normally located in the cellular cytoplasm, where they generate ATP to power the cell's functions.

Dynamically directing cell organization via micro-hump structure patterned cell-adhered interfaces.

Cell adhesion plays an important role in cell communication, organ formation and tissue maintenance. Spatial microstructure patterning has the capability to regulate cell functions such as cell adhesion and cell proliferation as well as cellular mechanical properties. In this study, we present a simple method to fabricate micro-hump patterned interfaces based on electrohydrodynamic jet (E-jet) printing to control and direct cell organization.

Functional Segregation of the Middle Temporal Visual Motion Area Revealed With Coactivation-Based Parcellation.

Traditionally, the visual motion area (MT) is considered as a brain region specialized for visual motion perception. However, accumulating evidence showed that MT is also related to various functions, suggesting that it is a complex functional area and different functional subregions might exist in this area. To delineate functional subregions of this area, left and right masks of MT were defined using meta-analysis in the BrainMap database, and coactivation-based parcellation was then performed on these two masks.

Indole alkaloid glycosides with a 1'-(phenyl)ethyl unit from leaves.

Seven indole alkaloid glycosides containing a 1'-(4″-hydroxy-3″,5″-dimethoxyphenyl)ethyl unit (-) were isolated from an aqueous extract of leaves (da qing ye). Their structures were determined by spectroscopic data analysis combined with enzymatic hydrolysis as well as comparison of their experimental CD (circular dichroism) and calculated ECD (electrostatic circular dichroism) spectra. Based on analysis of and/or Cotton effect (CE) data of -, two simple roles to assign location and/or configuration of -glycopyranosyloxy and 1'-(phenyl)ethyl units in the indole alkaloid glycosides are proposed.

Hepatic adrenal adenoma-rare tumor on right lobe of liver: a case report and literature review.

Background: Hepatic adrenal ectopia is a common clinical diagnosis, whereas adrenal tumors developed from hepatic adrenal ectopia are rare. Hepatic adrenal tumors are easily misdiagnosed as hepatic carcinoma and frequently treated by unnecessary operations.

Case Presentation: A 50-year-old female patient was hospitalized due to B-ultrasonic detection of "right focal liver lesions.

Effects of oxymatrine on the proliferation of human liver cancer Bel-7404 cells: A protocol of systematic review and meta-analysis.

Background: This study will examine the effects of oxymatrine on the proliferation of human liver cancer Bel-7404 cells (HLCBC).

Methods: This study will search electronic bibliographic databases available in PUBMED, EMBASE, Cochrane Library, Scopus, Cumulative Index to Nursing and Allied Health Literature, China Biology Medicine, and China National Knowledge Infrastructure. We attempt to search case-controlled studies (CCSs) or randomized controlled studies (RCSs) pertaining to HLCBC from their inception to the February 29, 2020 without limitations of language and publication time.

Generation of Multipotent Stem Cells from Adult Human Peripheral Blood Following the Treatment with Platelet-Derived Mitochondria.

Autologous stem cells are highly preferred for cellular therapy to treat human diseases. Mitochondria are organelles normally located in cytoplasm. Our recent studies demonstrated the differentiation of adult peripheral blood-derived insulin-producing cells (designated PB-IPC) into hematopoietic-like cells after the treatment with platelet-derived mitochondria.

The association between Ki-67 expression and the clinical pathological characteristics of colorectal cancer: A protocol for a systematic review and meta-analysis.

Background: This study will explore the association between Ki-67 expression and clinical pathological characteristics (CPC) of colorectal cancer (CC).

Methods: We will search relevant studies from electronic databases (Cochrane Library, PUBMED, EMBASE, Scopus, Cumulative Index to Nursing and Allied Health Literature, China Biology Medicine, and China National Knowledge Infrastructure) from beginning to April 1, 2020 without language and publication time limitations. We will consider all case-controlled studies (CCSs) or randomized controlled studies (RCSs) investigating the association between Ki-67 expression and CPC of CC.

Enhanced high-quality super-resolution imaging in air using microsphere lens groups.

Most microsphere-assisted super-resolution imaging experiments require a high-refractive-index microsphere to be immersed in a liquid to improve the super-resolution. However, samples are inevitably polluted by residuals in the liquid. This Letter presents a novel (to the best of our knowledge) method employing a microsphere lens group (MLG) that can easily achieve high-quality super-resolution imaging in air.

Deciphering the crucial roles of AraC-type transcriptional regulator Cgl2680 on NADPH metabolism and L-lysine production in Corynebacterium glutamicum.

Lysine is widely used in food, medical and feed industries. The biosynthesis of L-lysine is closely related to NADPH level, but the regulation mechanism between the biosynthesis of L-lysine in C. glutamicum and the cofactor NADPH is still not clear.

Effects of artemisinin on proliferation and apoptosis of human liver cancer HepG2 cells: A protocol of systematic review and meta-analysis.

Background: This study will examine the effects of artemisinin on proliferation and apoptosis of human liver cancer HepG2 cells (HLCHG-2C).

Methods: This study will systematically retrieve potential literatures in MEDLINE, Scopus, Web of Science, Cochrane Library, EMBASE, WANGFANG, and China National Knowledge Infrastructure from their initiation to the February 29, 2020. There are not limitations related to the language and publication time.

Effects of cinnamaldehyde on anti-respiratory syncytial virus: A protocol of systematic review and meta-analysis.

Background: Previous reports found that cinnamaldehyde has effects on anti-respiratory syncytial virus (ARSV). However, their results are still contradictory. Therefore, this study will systematically address the effects of cinnamaldehyde on ARSV.

Clinicopathological significance of Ki67 expression in colorectal cancer: A protocol of systematic review and meta-analysis.

Background: This study will investigate the diagnostic accuracy of Ki67 expression in colorectal cancer (CC).

Methods: A comprehensive search in electronic bibliographic databases (MEDLINE, EMBASE, Cochrane Library, Web of Science, Chinese Biomedical Literature Database, and China National Knowledge Infrastructure) will be performed from inception to the February 29, 2020 with no restrictions to the language and publication status. Two authors will examine the collected studies, extract essential data, and appraise study quality separately.

AMP-activated protein kinase in the grass carp Ctenopharyngodon idellus: Molecular characterization, tissue distribution and mRNA expression in response to overwinter starvation stress.

Adenosine monophosphate-activated protein kinase (AMPK) is the main energy sensor in mammals, but limited information is available regarding its role as an energy sensor in nutrient-restricted fish particularly in period of overwinter starvation. The present study aimed to investigate the role of AMPK in the grass carp Ctenopharyngodon idellus through characterization of AMPK full-length cDNAs and the measurement of transcriptional activity in response to overwinter starvation. AMPK is a heterotrimeric serine/threonine kinase that consists of a catalytic alpha (α) subunit complexed with two regulatory subunits, beta (β) and gamma (γ).

Different Acupuncture Therapies for Allergic Rhinitis: Overview of Systematic Reviews and Network Meta-Analysis.

Objective: To evaluate the quality of methodologies used in previous systematic reviews (SRs) and compare efficacy of different acupuncture therapies for allergic rhinitis.

Methods: Seven electronic databases were searched for systematic reviews (SRs) performed on different acupuncture therapies for allergic rhinitis from inception to 15 November 2019. The AMSTAR2 instrument was employed to assess the methodological quality of included SRs.

Self-Interacting Dark Matter Subhalos in the Milky Way's Tides.

We study evolution of self-interacting dark matter subhalos in the Milky Way tidal field. The interaction between the subhalos and the Milky Way's tides lead to more diverse dark matter distributions in the inner region, compared to their cold dark matter counterparts. We test this scenario with two Milky Way satellite galaxies, Draco and Fornax, opposite extremes in the inner dark matter content, and find that they can be accommodated within the self-interacting dark matter model proposed to explain the diverse rotation curves of spiral galaxies in the field.

Efficacy Comparison of Different Acupuncture Treatments for Functional Dyspepsia: A Systematic Review with Network Meta-Analysis.

Background: Acupuncture has been found to be an effective treatment for functional dyspepsia (FD). Currently, several types of acupuncture have been developed but it is not clear which type is suitable for FD. Currently, doctors often rely on experience to decide which form of acupuncture to apply.

Cross-scale additive direct-writing fabrication of micro/nano lens arrays by electrohydrodynamic jet printing.

High-quality micro/nanolens arrays (M/NLAs) are becoming irreplaceable components of various compact and miniaturized optical systems and functional devices. There is urgent requirement for a low-cost, high-efficiency, and high-precision technique to manufacture high-quality M/NLAs to meet their diverse and personalized applications. In this paper, we report the one-step maskless fabrication of M/NLAs via electrohydrodynamic jet (E-jet) printing.

A new class of quadruplex DNA-binding nickel Schiff base complexes.

We have prepared six new nickel Schiff base complexes via reactions of substituted benzophenones with different diamines in the presence of nickel(ii). These new complexes were then reacted with 1-(2-choroethyl)piperidine to afford a further six novel nickel(ii) Schiff base complexes bearing pendant ethylpiperidine groups. The complexes bearing the ethylpiperidine moieties had greater solubility in water, and were therefore suitable for use in DNA binding experiments.

Existence of Circulating Mitochondria in Human and Animal Peripheral Blood.

Mitochondria are usually located in the cytoplasm of cells where they generate adenosine triphosphate (ATP) to empower cellular functions. However, we found circulating mitochondria in human and animal blood. Electron microscopy confirmed the presence of mitochondria in adult human blood plasma.

Released Exosomes Contribute to the Immune Modulation of Cord Blood-Derived Stem Cells.

Clinical studies demonstrated the immune modulation of cord blood-derived stem cells (CB-SC) for the treatment of type 1 diabetes and other autoimmune diseases, with long-lasting clinical efficacy. To determine the molecular mechanisms underlying the immune modulation of CB-SC, the actions of exosomes released from CB-SC were explored in this study. Exosomes were isolated from CB-SC cultures using ultracentrifugation and confirmed with different markers.

Functional MRI Investigation of Ultrasound Stimulation at ST 36.

Background: Clinical and experimental data suggest that ultrasound stimulation (US) at acupoints can produce similar effective treatment compared to manual acupuncture (MA). Although the brain activation to MA at acupoints is investigated by numerous studies, the brain activation to US at acupoints remains unclear.

Methods: In the present work, we employed task state functional magnetic resonance imaging (fMRI) to explore the human brain's activation to US and MA at ST 36 () which is one of the most commonly used acupoints in acupuncture-related studies.

Automatic Facial Paralysis Assessment via Computational Image Analysis.

Facial paralysis (FP) is a loss of facial movement due to nerve damage. Most existing diagnosis systems of FP are subjective, e.g.

Metabolic engineering of carbohydrate metabolism systems in Corynebacterium glutamicum for improving the efficiency of L-lysine production from mixed sugar.

The efficiency of industrial fermentation process mainly depends on carbon yield, final titer and productivity. To improve the efficiency of L-lysine production from mixed sugar, we engineered carbohydrate metabolism systems to enhance the effective use of sugar in this study. A functional metabolic pathway of sucrose and fructose was engineered through introduction of fructokinase from Clostridium acetobutylicum.