Engineered hsa-miR-455-3p-Abundant Extracellular Vesicles Derived from 3D-Cultured Adipose Mesenchymal Stem Cells for Tissue-Engineering Hyaline Cartilage Regeneration.

Efforts are made to enhance the inherent potential of extracellular vesicles (EVs) by utilizing 3D culture platforms and engineered strategies for functional cargo-loading. Three distinct types of adipose mesenchymal stem cells-derived EVs (ADSCs-EVs) are successfully isolated utilizing 3D culture platforms consisting of porous gelatin methacryloyl (PG), PG combined with sericin methacryloyl (PG/SerMA), or PG combined with chondroitin sulfate methacryloyl (PG/ChSMA). These correspond to PG-EVs, PG/SerMA-EVs, and PG/ChSMA-EVs, respectively.

Semi-synthesis and structural elucidation of brevicanines A-D, four new C-diterpenoid alkaloids with rotameric phenomenon from Aconitum brevicalcaratum.

Four new C-diterpenoid alkaloids brevicanines A-D (1-4) with rotameric phenomenon were isolated from Aconitum brevicalcaratum. They all possessed an unusual axial chiral phenyl-quinazoline side chain and their structures were elucidated by extensive spectroscopic analysis and chemical methods. Meanwhile, brevicanines A and B were semi-synthesized from their parent compound scaconine to further confirm their structures.

Differential expression of long noncoding RNAs in congenital microtia.

Objective: To analyse lncRNA expression profiles in microtia using bioinformatics analysis.

Methods: We examined lncRNA expression profiles in residual ear cartilage and normal ear cartilage from individual congenital microtia patients.

Results: The gene chips used in this study included 30586 lncRNAs and 26109 mRNA probes.

[Correction of cryptotia using crescent flap with skin-grafting].

Objective: To investigate the therapeutic effect of crescent flap with skin-grafting for the correction of cryptotia.

Methods: Between the helix and scalp, we designed a crescent flap with the pedicle above the helix. Another affiliated triangular flap was formed below the crescent flap.

κ-carrageenan induces the disruption of intestinal epithelial Caco-2 monolayers by promoting the interaction between intestinal epithelial cells and immune cells.

κ-carrageenan (κ-CGN) is an important food additive that has been demonstrated to induce colitis in animal models. In the present study, the effects of κ-CGN were assessed using an in vitro co-culture system that contained intestinal epithelial Caco-2 cells and activated macrophage-like THP-1 cells. The results demonstrated that in single cultures of Caco-2 and THP-1 cells treated with κ-CGN, the cytotoxicity and the secretion levels of IL-1β, IL-6 and TNF-α were low.

[Ear reconstruction after rupture of postauricular expanded flap].

Objective: To summarize the principles of ear reconstruction after rupture of postauricular expanded flap.

Methods: The rupture of the postauricular expanded flap was graded as I-IV degree. For flap rupture of I and II degree, the ear reconstruction was performed immediately with autogenous cartilage framework.

[Reconstruction of traumatic ear defect by retro-auricular expanded flap and cartilage graft].

Objective: To investigate the therapeutic effect of retro-auricular expanded flap and cartilage graft for reconstruction of traumatic ear defect.

Methods: From Aug. 2008 to Aug.

[Experimental research on DNA methylation profile in congenital microtia].

Objective: To screen for abnormal methylation in CpG islands and CpG sites through whole genome of congenital microtia to identify their associated genes. To discuss the relationship between abnormal methylation level of genes and the etiology of congenital microtia.

Methods: Residual ear cartilage of 50 patients with microtia was collected with ear cartilage of 34 patients without ear malformations as control.

[Sectional anatomical analysis of auricular and middle ear malformation in patients with microtia].

Objective: To analyze the sectional anatomical features of auricular and middle ear malformation in patients with microtia so as to improve the clinical classification and the instruction of surgery.

Methods: From Jun. to Dec.

[Application of bi-pedicled frontal expanded flap for cervical cicatricial contracture].

Objective: To investigate the therapeutic effect of bi-pedicled frontal expanded flap for cervical cicatricial contracture.

Methods: Tissue expanders were implanted under frontal muscle. After expansion, the frontal flaps were designed based on bilateral superficial temporal vessels and were transferred to the neck wound.

[Study of methylation of promoter of EYA1 gene in microtia].

Objective: To explore the methylation of CpG islands in promoter of eye absent gene 1 (EYA1) in microtia.

Methods: The methylation of CpG islands in EYA1 gene in 64 microtias and 36 healthy controls were measured using the technique of matrix-assisted laser desorption/ionization-time of flight.

Results: The methylation of CpG_Unit3 and CpG_Unit5 of EYA1 gene in microtia were 0.

[Surgical treatment and etiological analysis of polyotia].

Objective: To explore the surgical treatment and pathologic mechanism of polyotia.

Methods: The operative methods for polyotia were flexible. The main procedures were aimed at the remodeling of tragus with the redundant tissue.

[Application of quantitative tissue expansion in ear reconstruction].

Objective: To explore the application of tissue expander in ear reconstruction and to deal with the complications.

Methods: 50 ml kidney-shape tissue expanders were implanted subcutaneously in the mastoid area. The drainage tube was removed 3 days after operation.

[Application of the remnant in repairing the reconstructed ear in microtia].

Objective: To explore the application of the remnant in repairing the reconstructed ear in microtia.

Methods: From October 2000 to July 2006, 1,823 patients with reconstructed ear underwent the second operation for repairing. The remnants were used to reconstruct tragus, antitragus, crus of helix, concha cavum, or relax the adhesion between the reconstructed auricle and cranio wall, or insert into the reconstructed lobule or under the basement of the costal cartilage frame to restore the cranio-auricular angle or to the preauricular subcutaneous to correct the facial local depression.

[Fabrication and application of three-dimensional autogenous cartilage framework in auricular reconstruction].

Objective: To explore the fabrication and application of three-dimensional autogenous cartilage framework in auricular reconstruction.

Methods: The process of fabrication of three-dimensional cartilage framework consisted of cartilage harvesting, carving and assembling the cartilage. The rib cartilage was harvested separately.

[Clinical study of chest contour deformity after harvesting of costal cartilage for total ear reconstruction].

Objective: To investigate the chest contour deformities after harvesting of costal cartilage for total ear reconstruction, so as to prevent donor site morbidity.

Methods: 107 cases of microtia who underwent ear reconstruction with costal cartilage during the period from 1994 to 2003, were retrospectively studied. Both the chest depression deformity and the costal arch deformity were evaluated.

[Clinical application of three methods for total ear reconstruction].

Objective: To investigate the indication and results of three methods for total ear reconstruction.

Methods: 960 cases of total ear defect were treated with skin expansion and autogenous rib cartilage framework (n = 786), or skin expansion and Medpor framework (n = 150), or ear prosthesis (n = 24). The indication and results of the three methods were analysed.

[Clinical significance of vascular distribution type of the expanded skin in the mastoid region in congenital microtia].

Objective: To classify the blood vessel distribution of the expanded skin in the mastoid region and its relevance to reasonable and reliable design of the expanded flap for auricular reconstruction in congenital microtia.

Methods: The blood vessel distribution of the expanded skin in the mastoid region was observed by light permeation test. The expanded flaps with different ratio of length to width were designed according to their blood vessel distribution types.

[Surgical treatment of acquired ear defect].

Objective: To explore surgical procedure for the treatment of acquired ear defect.

Methods: The ear reconstruction was carried out by using soft tissue skin expander and autogenous rib cartilage framework, Medpor framework, auricular prosthesis, and so on.

Results: The long-term follow-ups showed that the flap of reconstructed ear was ruddy, soft, with normal sensory function.

[Classification and repairment of "butterfly ear" deformity].

Objective: To classify and repair "Butterfly Ear" deformity which presents characters of dysplasia of inferior auricle of ear and congenital bat ear.

Method: The repairment procedures include: type I: auricular cartilage flap inversion folding technique. type II: local ear skin flap.

[Ear reconstruction using soft tissue expander in the treatment of congenital microtia].

Objective: To explore surgical procedure of congenital microtia.

Methods: Ear reconstruction was made using soft tissue skin expander and autogenous rib cartilage framework.

Results: Long time follow-up showed that the flap of reconstructed ear was ruddy, soft, with normal sensory function; cartilage framework had no degeneration, absorption and deformation.

[Psychologic status and their influencing factors in congenital microtia patients and their families].

Objective: To study the psychologic status and their influencing factors in congenital microtia patients and their families.

Methods: Totally one hundred and two congenital microtia patients (79 men, 23 women, mean age 13.62 +/- 7.