[The impact of HLA high resolution typing mismatching of donor-recipient pairs on outcome of unrelated donor hematopoietic stem cell transplantation].

Objective: To study the impact of various human leukocyte antigen (HLA) high resolution typing mismatching of donor-recipient pairs on prognosis of unrelated donor hematopoietic stem cell transplantation.

Methods: 835 donor-recipient pairs of CMDP data from 2005 to 2010 were analyzed retrospectively. HLA-A, B, C, DRB1 and DQB1 typing were performed using SBT, SSOP and SSP methods.

[A study on apoptosis and apoptotic mechanisms of HL-7702 cell line induced by methylmercury].

Objective: To study the apoptotic effect and mechanisms of methylmercury (MeHg) on HL-7702 cell line in vitro.

Methods: In this study, the cell apoptosis was observed by AO/EB method and FCM method; the mitochondrial membrane potential was detected by FCM; and the expression of proteins related to apoptosis was measured by immunocytochemical method.

Results: After exposure to MeHg for 24 h in different doses, apoptotic rate ascended with the increasing of MeHg concentration.

[Toxicity of nano silica particles on rat type I-like alveolar epithelial cells].

Objective: To access rat lung toxicity of nano silica particles.

Methods: Transmission electron microscope was used to observe size, shape and dispersibility of two silica particles; Size of two particles in water and RPMI 1640 containing 1% FBS were measured using Zeta Potential Analyzer. LDH activities of rat type I-like alveolar epithelial cell line R3/1 cells after 6, 24 and 48 h incubation with 2.

[Effects of over-expressed Smac gene coupling with cisplatin on proliferation and apoptosis of hepatocarcinoma cells].

Objective: To investigate the effects of over-expressed Smac gene combined with cisplatin (CDDP) on proliferation and apoptosis of hepatic carcinoma cells.

Methods: The recombinant plasmid pcDNA3.1+-hSmac was introduced into the human hepatic carcinoma SMMC-7721 cells using a liposome-mediated method.