[Characteristics, Sources, and Contributions to Ozone Formation of Ambient Volatile Organic Compounds in Huanggang, China].

Based on the offline sampling data of volatile organic compounds (VOCs) and the simultaneous online measurements of conventional gaseous air pollutants and meteorological parameters in urban Huanggang, the volume fractions and component characteristics of VOCs were analyzed. The sources and ozone (O) formation sensitivity of VOCs during severe ozone pollution episodes were analyzed using the positive matrix factorization (PMF) model and the photochemical box model coupled with master chemical mechanism (PBM-MCM), respectively. The results revealed that the average volume fractions of total volatile organic compounds were (21.

[Pollution Characteristics and Source Analysis of Carbon and Nitrogen Components in Ambient PM in Huangshi City].

Based on stable isotope technology and a PMF model, the pollution characteristics and sources of carbon and nitrogen components in ambient PM in Huangshi City were explored. The results showed that the total carbon concentration[(TC)] and the total carbon isotopic composition (C) in ambient PM in Huangshi City both showed seasonal variation characteristics of being high in winter and low in summer, with values of (4.4±1.

[Effect of WESP on Emission Characteristics of Condensable Particulate Matter from Ultra-low Emission Coal-fired Power Plants].

In order to study the effect of wet electrostatic precipitators(WESP) on emission characteristics of condensable particulate matter (CPM) from ultra-low emission coal-fired power plants that are under different capacity conditions, a set of CPM sampling devices was built based on US EPA Method 202, and an ultra-low emission coal-fired power plant was detected. This study evaluated the emission level of the CPM from the flue gas of coal-fired power plants, the effects of different unit capacity conditions on the CPM emission concentrations, and the removal efficiency of WESP for different components of the CPM. The results suggested that the emission concentrations of the CPM from ultra-low emission power plants were 27.

[Source Analysis of Ambient PM in Wuhan City Based on Random Forest Model].

Based on the online monitoring data of fine particle(PM) mass concentration, carbonaceous components, ionic constituents, and elemental components in an urban site of Wuhan from December 2019 to November 2020, the chemical characteristics of PM were analyzed. In addition, seasonal source apportionment of PM was conducted using the principal component analysis(PCA) method and random forest(RF) algorithm model. The results indicated that (PM) was the highest in winter[(61.

[Characteristics of Volatile Organic Compounds in Wood Coatings and Automotive Coatings in China].

Presently, there are a few studies on the measurement of industrial organic solvents in China. To determine the content and species of volatile organic compounds (VOCs) in industrial organic solvents and to provide the emission factors of organic solvents, the Chinese wood and automotive coatings, which accounted for the largest proportion of production in the world, were investigated. Coating samples were obtained by sampling from companies and buying from markets, and were measured in accordance with the domestic standard testing methods for coatings.

Seasonal variations and chemical characteristics of PM(2.5) in Wuhan, central China.

PM2.5 samples were collected at an urban site (WD) and a suburban site (TH) in Wuhan from August 2012 to July 2013. The mass concentrations of water-soluble inorganic ions, carbonaceous species and elements of PM2.

Polycyclic aromatic hydrocarbons (PAHs) in the air of Chinese cities.

Polycyclic aromatic hydrocarbons (PAHs) were determined in the air of 37 cities and 3 rural locations across China during the winter, spring, summer and autumn of 2005, using polyurethane foam (PUF) disks as passive air samplers (PAS). Winter and autumn concentrations in cities exceeded spring and summer values. Concentrations were amongst the highest in the world; seasonally averaged autumn/winter values in some cities in the north and north-west of China exceeded proposed European Union air quality standards.

Cloning, sequencing and characterization of the alpha-aminoadipate reductase gene (LYS2) from Saccharomycopsis fibuligera.

The gene putatively encoding alpha-aminoadipate reductase (AAR) was isolated successfully by degenerate PCR and chromosome walking, based on cassette PCR methods, from the dimorphous yeast Saccharomycopsis fibuligera PD70 and was named SfLYS2. Sequence analysis revealed that it contained a putative open reading frame (ORF) of 4161 bp and encoded a polypeptide of 1386 amino acids. The deduced translation product shared an identity of 53% and 51% to the Lys2p homologues of Candida albicans and Saccharomyces cerevisiae, respectively.

Extremely rapid extraction of DNA from bacteria and yeasts.

A very simple and rapid method for extracting genomic DNA from Gram-negative bacteria, Gram-positive bacteria and yeasts is presented. In this method, bacteria or yeasts are lysed directly by phenol and the supernatant is extracted with chloroform to remove traces of phenol. The supernatant contains DNA that is suitable for molecular analyses, such as PCR, restriction enzyme digestion and genomic library construction.