Publications by authors named "Hai-jun Deng"
Background: Transanal total mesorectal excision (TaTME) allows patients with ultralow rectal cancer to be treated with sphincter-saving surgery. However, accurate delineation of the distal resection margin (DRM), which is essential to achieve R0 resection for low rectal cancer in TaTME, is technically demanding.
Aim: To assess the feasibility of optical biopsy using probe-based confocal laser endomicroscopy (pCLE) to select the DRM during TaTME for low rectal cancer.
Article Synopsis
- A study was conducted to compare the short-term effectiveness of laparoscopic surgery versus open surgery for treating low rectal cancer, involving 1,070 patients randomized across 22 hospitals in China.
- The results showed similar rates of complete mesorectal excision between the two techniques, with minor differences in the rates of negative margins and lymph nodes retrieved.
- Overall, the findings indicate that laparoscopic surgery may have comparable outcomes to open surgery for low rectal cancer, but the differences in measures like sphincter preservation were not detailed.
Protein sensors combining both on-and-off model for antibody homogeneous assay.
Biosens Bioelectron
August 2022
Article Synopsis
- Researchers have developed a new protein sensor called NanoSwitch, based on the modularized luciferase NanoLuc, that can detect antibodies in serum without washing steps, achieving results in just 45 minutes.
- NanoSwitches exhibit high signal-to-noise ratios (S/N), reaching up to 42-fold for specific antibodies and over 200-fold for SARS-CoV-2 antibodies, allowing for effective differentiation between signals and background noise in a variety of serum samples.
- The innovative design combines a turn-off mechanism with human serum albumin and a turn-on response triggered by specific antibodies, indicating potential for better sensor performance in detecting various pathogens like HIV and HCV.
Background And Aims: The mechanism underlying HCC metastasis remains unclear, many oncogenes are known to regulate this process. However, the role of alternative splicing (AS) in pro-metastatic HCC is poorly understood.
Approach And Results: By performing RNA sequencing on nine pairs of primary HCC tissues with extrahepatic metastasis (EHMH) and nine pairs of metastasis-free HCC (MFH) tissues, we depicted the AS landscape in HCC and found a higher frequency of AS events in EHMH compared with MFH.
Article Synopsis
- The study investigated the clinical features of children infected with COVID-19 compared to other respiratory infections like RSV, Influenza, and adenovirus, focusing on symptoms and cytokine profiles.
- It involved 20 COVID-19 positive hospitalized children, 58 with other acute respiratory tract infections, and 20 healthy controls, with data collected on clinical symptoms and blood test results.
- Findings revealed that COVID-19 patients were older and had milder symptoms than those with other infections, along with notable differences in immune response indicated by cytokine levels.
Article Synopsis
- The study assesses the long-term durability of the immune response to SARS-CoV-2 about six months after infection, focusing on memory B cells and T cells.
- Memory B cells specific to the virus's spike protein were found in low numbers in recovered individuals, particularly in those who had asymptomatic cases.
- T cell responses were observed in both recovered COVID-19 patients and some healthy individuals, indicating potential cross-reactive immunity.
Background?>: Laparoscopic surgery for rectal cancer is commonly performed in China. However, compared with open surgery, the effectiveness of laparoscopic surgery, especially the long-term survival, has not been sufficiently proved.
Methods?>: Data of eligible patients with non-metastatic rectal cancer at Nanfang Hospital of Southern Medical University and Guangdong Provincial Hospital of Chinese Medicine between 2012 and 2014 were retrospectively reviewed.
Article Synopsis
- COVID-19 is caused by SARS-CoV-2, and this study explores the levels of neutralizing antibodies (NAbs) in patients over a three-month period after symptoms begin.
- Antibody levels were low during the first week but peaked around 33 days post-symptom onset, with a significant majority showing a gradual decline in NAb levels over three months.
- NAb levels were found to be positively correlated with immunoglobulin G (IgG) and specific proinflammatory cytokines, indicating a relationship between immune response and antibody dynamics.
The clinical features and immune responses of asymptomatic individuals infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have not been well described. We studied 37 asymptomatic individuals in the Wanzhou District who were diagnosed with RT-PCR-confirmed SARS-CoV-2 infections but without any relevant clinical symptoms in the preceding 14 d and during hospitalization. Asymptomatic individuals were admitted to the government-designated Wanzhou People's Hospital for centralized isolation in accordance with policy.
View Article and Find Full Text PDFArticle Synopsis
- Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes COVID-19 and is primarily diagnosed using real-time RT-PCR, which has some accuracy limitations.
- Researchers developed a new peptide-based luminescent immunoassay that detects IgG and IgM antibodies to improve detection.
- The immunoassay showed positive rates of 71.4% for IgG and 57.2% for IgM in confirmed patients, suggesting it could enhance COVID-19 diagnosis when used alongside RT-PCR.
Article Synopsis
- In December 2019, COVID-19 caused by the novel coronavirus emerged in Wuhan, China, with limited information on its clinical and immunological effects in children.
- A study analyzed twelve pediatric COVID-19 patients, revealing that common symptoms were cough (75%) and fever (58.3%), while children exhibited a longer incubation period (average of 8 days) compared to adults.
- The immune profiles indicated that children had higher levels of T cells and B cells, which may relate to their milder symptoms, and it is recommended that testing for SARS-CoV-2 be conducted for children with potential exposure, regardless of symptoms.
Article Synopsis
- The study analyzed the antibody responses of 285 COVID-19 patients, finding that all tested positive for IgG within 19 days of symptom onset.
- IgG and IgM antibodies either developed at the same time or one after the other, reaching stable levels within 6 days post-seroconversion.
- Serological tests could aid in diagnosing COVID-19, especially in cases with negative RT-PCR results and in detecting asymptomatic infections.
The diverse expression pattern of CD36 reflects its multiple cellular functions. However, the roles of CD36 in colorectal cancer (CRC) remain unknown. Here, we discover that CD36 expression is progressively decreased from adenomas to carcinomas.
View Article and Find Full Text PDFElevated levels of neutrophils have been associated with poor survival in various cancers, but direct evidence supporting a role for neutrophils in the immunopathogenesis of human cancers is lacking. A total of 573 patients with gastric cancer were enrolled in this study. Immunohistochemistry and real-time PCR were performed to analyze the distribution and clinical relevance of neutrophils in different microanatomic regions.
View Article and Find Full Text PDF[TALEN-mediated MYH9 Knock-down and its influence on cell cycle and apoptosis of MGC803 cell line].
Nan Fang Yi Ke Da Xue Xue Bao
March 2016
Objective: To construct a MYH9 gene knockout model in MGC803 cell line using transcription activator-like effector nuclease (TALEN) and observe its effect on cell cycle and apoptosis.
Methods: According to FastTALE(TM) TALEN Kit, we designed TALEN pairs and constructed the plasmids targeting to MYH9 gene. After detecting their activity in MGC803 cells by plasmid transfection, DNA sequencing, RT-PCR and western blot, we selected the monoclonal cells and studied the changes in the cell cycle and apoptosis.
Article Synopsis
- Elevated S100P expression is linked to colorectal cancer (CRC) metastasis and poor prognosis, being a more effective predictor than traditional markers like CEA and CA19-9.
- High S100P levels were shown to enhance the invasiveness and ability of CRC cells to metastasize, supported by experiments involving stable S100P knockdown and xenograft growth in mice.
- The process by which S100P promotes cancer progression involves activating the RAGE/ERK signaling pathway and inducing epithelial-mesenchymal transition (EMT), with RAGE being essential for this effect in colon cancer.
[Laparoscopic diagnosis and treatment of rectum telangiectasia: report of a rare form of lower gastrointestinal hemorrhage].
Nan Fang Yi Ke Da Xue Xue Bao
October 2015
We report a case of rectum telangiectasia, a rare form of lower gastrointestinal hemorrhage caused by vascular malformation. The patient underwent laparoscopic assisted abdomino-perineal resection of the sigmoid colon-rectum telangiectasia. The extent and degree of pathological changes were observed directly from the intestinal wall during laparoscopic surgery, and after collection of biopsy evidence, concomitant definitive surgery was performed to achieve a minimally invasive effect.
View Article and Find Full Text PDFThere are significant differences in clinical characteristics between chronic hepatitis B virus infected (CHB) child and adult patients. Viral quasispecies characteristics are associated with its pathogenic properties. For hepatitis B virus (HBV), its core region is the main immune recognition region for its enriched epitopes.
View Article and Find Full Text PDFAim: To evaluate the 5-year survival after laparoscopic surgery vs open surgery for stages II and III rectal cancer.
Methods: This study enrolled 406 consecutive patients who underwent curative resection for stages II and III rectal cancer between January 2000 and December 2009 [laparoscopic rectal resection (LRR), n = 152; open rectal resection (ORR), n = 254]. Clinical characteristics, operative outcomes, pathological outcomes, postoperative recovery, and 5-year survival outcomes were compared between the two groups.
Techniques and feasibility of laparoscopic extended right hemicolectomy with D3 lymphadenectomy.
World J Gastroenterol
August 2014
Aim: To illustrate the critical techniques and feasibility of laparoscopic extended right hemicolectomy (LERH), according to our previous experience.
Methods: Anatomical relationship and operative techniques were demonstrated. One hundred and five consecutive patients who underwent extended right hemicolectomy with D3 lymphadenectomy between January 2008 and May 2011 were included in the present study [laparoscopic group (n = 48) vs open group (n = 57)].
Anti-miRNA-221 sensitizes human colorectal carcinoma cells to radiation by upregulating PTEN.
World J Gastroenterol
December 2013
Aim: To investigate the regulative effect of miRNA (miR)-221 on colorectal carcinoma (CRC) cell radiosensitivity and the underlying mechanisms.
Methods: A human CRC-derived cell line was cultured conventionally and exposed to different doses of X-rays (0, 2, 4, 6 and 8 Gy). The total RNA and protein of the cells were extracted 24 h after irradiation, and the alteration of miR-221 and phosphatase and tensin homolog deleted on chromosome 10 (PTEN) gene mRNA expression was detected by real-time reverse transcriptase polymerase chain reaction (PCR).
Objective: To investigate the regulative effect of microRNA-338-3p on colorectal carcinoma cell invasion and migration.
Methods: The microRNA-338-3p expression pattern of colorectal carcinoma tissues and cell lines was detected by real-time reverse transcriptase polymerase chain reaction. The protein level of smoothened was detected by western blot analysis.