Background: The co-culture strategy which mimics natural ecology by constructing an artificial microbial community is a useful tool to activate the biosynthetic gene clusters to generate new metabolites. However, the conventional method to study the co-culture is to isolate and purify compounds separated by HPLC, which is inefficient and time-consuming. Furthermore, the overall changes in the metabolite profile cannot be well characterized.
View Article and Find Full Text PDFFive new indole-terpenoids named penerpenes E-I (-), along with seven known ones (-), were isolated from the marine-derived fungus sp. KFD28 from a bivalve mollusk, . The structures of the new compounds were elucidated from spectroscopic data and ECD spectroscopic analyses.
View Article and Find Full Text PDFFour unusual indole-terpenoids, penerpenes A-D (1-4), along with two known ones paxilline (5) and emindole SB (6), were isolated from the marine-derived fungus Penicillium sp. KFD28. The absolute structures of 1-4 were elucidated on the basis of spectroscopic data and ECD spectra analysis along with quantum ECD calculations.
View Article and Find Full Text PDFBackground: Tisp40, a transcription factor of the CREB/CREM family, is involved in cell proliferation, differentiation and other biological functions, but its role in renal tubulointerstitial fibrosis is unknown.
Methods: In our study, we investigated the effects of Tisp40 on extracellular matrix (ECM) accumulation, epithelial-mesenchymal transition (EMT) and the underlying molecular mechanisms in transforming growth factor-β (TGF-β)-stimulated TCMK-1 cells by quantitative real-time polymerase chain reaction (qPCR), Western blot analysis and immunofluorescence in vitro, and further explored the role of Tisp40 on renal fibrosis induced by ischemia-reperfusion (I/R) by qPCR, Western blot analysis, hydroxyproline analysis, Masson trichrome staining and immunohistochemistry staining in vivo.
Results: The data showed that Tisp40 was upregulated in a model of renal fibrosis induced by I/R injury (IRI).
Wei Sheng Wu Xue Bao
December 2004
Firstly, the complete gB gene of a domestic isolation stain were amplified by PCR, and the 2.6 kb gene fragment was obtained. Then the recombinant plasmid pY-alpha-gB was constructed by cloning PCR product into pY-alpha vector, and the shuttle expression plasmid pR-alpha-gB was constructed by cloning the hsp-alpha-gB gene into the downstream sequences of pRR3 vector.
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