Development of a nanobody-based immunoassay for the sensitive detection of fibrinogen-like protein 1.

Immune checkpoint inhibition is an important strategy in cancer therapy. Blockade of CTLA-4 and PD-1/PD-L1 is well developed in clinical practice. In the last few years, LAG-3 has received much interest as an emerging novel target in immunotherapy.

A conductive sodium alginate and carboxymethyl chitosan hydrogel doped with polypyrrole for peripheral nerve regeneration.

Polymer materials with electrically conductive properties have good applications in their respective fields because of their special properties. However, they usually exhibited poor mechanical properties and biocompatibility. In this work, we present a simple approach to prepare conductive sodium alginate (SA) and carboxymethyl chitosan (CMCS) polymer hydrogels (SA/CMCS/PPy) that can provide sufficient help for peripheral nerve regeneration.

[Identification on Xanthium sibiricum from different habitats by FTIR fingerprint patterns].

Objective: To establish an FTIR method to identify Xanthium sibiricum from different habitats.

Methods: FTIR spectra of Xanthium sibiricum from different habitats were analyzed,and the similarity of different fingerprint spectra and the chemical pattern recognition were calculated and analyzed according to the wave numbers of peaks.

Results: Different FTIR spectra of 10 different habitats of Xanthium sibiricum were obtained,and the similarities were all above 0.

[Identification on Solanum lyratum by FT-IR fingerprint patterns and similarity degree of different fingerprint patterns].

Objective: To provide a FT-IR new method to identify different habitats of Solanum lyratum.

Methods: Analyzed FT-IR patterns of Solanum lyratum from different habitats, and the similarity of different fingerprint patterns was calculated and analyzed according to the wave numbers of peaks searched.

Results: Obtained the different FT-IR pattern of 5 different habitats of Solanum lyraturn.

Preparation, characterization and in vitro release study of a glutathione-dependent polymeric prodrug Cis-3-(9H-purin-6-ylthio)-acrylic acid-graft-carboxymethyl chitosan.

In this work, an amphiphilic polymeric prodrug Cis-3-(9H-purin-6-ylthio)-acrylic acid-graft-carboxymethyl chitosan (PTA-g-CMCS) was designed and synthesized. In aqueous solution, this grafted polymer can self-assemble into spherical micelles with a size ranging from 104 to 285 nm and zeta potential ranging from -12.3 to -20.

[Identification on Solanum lyratum by X-ray diffraction Fourier fingerprint and similarity analysis].

Objective: To provide an X-ray diffraction (XRD) method for identifying different medicinal parts of Solanum lyratum.

Methods: Analyzed X-ray diffraction Fourier patterns of different parts of Solanum lyratum, and the similarity degree of different fingerprint was calculated and analyzed according to the position (2 theta value)of peaks searched.

Results: Different X-ray diffraction Fourier patterns of different medicinal parts of Solanum lyratum were obtained.

Topical anti-inflammatory and analgesic activity of kirenol isolated from Siegesbeckia orientalis.

Ethnopharmacological Relevance: Siegesbeckia orientalis has been traditionally used as a topical anti-inflammatory and analgesic agent.

Aims Of The Study: Current study was designed to explore the topical anti-inflammatory and analgesic effects of a constituent isolated from Siegesbeckia orientalis (Compositae), in order to validate its folk use.

Materials And Methods: Kirenol was isolated from ethanolic extract of Siegesbeckia orientalis.

In vitro and in vivo evaluation of the wound healing properties of Siegesbeckia pubescens.

Ethnopharmacological Relevance: Siegesbeckia pubescens (SP) has been traditionally used as a wound healing agent.

Aim Of The Study: Investigate in vitro and in vivo healing properties of SP extract.

Materials And Methods: The methanolic extract of SP was tested for the ability to stimulate the growth of mouse fibroblast NIH3T3 in vitro.

The tissue distribution in mice and pharmacokinetics in rabbits of oxaliplatin liposome.

A rapid, sensitive, and simple high-performance liquid chromatographic (HPLC) method with an ultraviolet detector (UV) has been developed for the determination of oxaliplatin in the plasma of rabbits and tissues of mice. The sample preparation was carried out by complexation with 0.5 mL of DETC (diethyl-dithiocarbamate) solution and extracted by ether and chloroform.

[Study on the inclusion technology of volatile oil in Shiquandabu pills].

Objective: To extract volatile oil from Shiquandabu pills and study technological process of its inclusion compound with beta-Cyclodextrin (beta-CD) to improve its stability.

Methods: The study was carried out with steam distillation and orthogonal design. The process conditions were studied by determining the utilization and ratio of oil from Shiquandabu pills volatile oil and extract ratio of in-clusion compound.

[Identification on Spora Lygodii by X-ray diffraction fingerprint spectra method].

Objective: To set up a new identification and analysis method of Spora Lygodii.

Method: To get the extracts of Spora Lygodii using methanol, chloroform and petroleum ether as solvent and the extracts were identified, analyzed by X-ray diffraction Fourier fingerprint spectra.

Results: Experiments and analysis were carried out on three samples.