[Effects of soy isoflavones and major active component genistein on the expression of ovarian estrogen receptor-α in rats].

Objective: To investigate the effects of soy isoflavones (SI) on the expression of estrogen receptor-α (ER-α) in senile rat ovaries and ovarian granulosa cell cultured in vitro treated with genistein, a major active component of SI.

Methods: The animal model of perimenopause rats was established by unforced aging. The animals were treated by intragastric administration (ig) with low (50 mg/kg), middle (158 mg/kg) and high (500 mg/kg) dose of SI for 8 weeks.

Expression of nucleophosmin in glandular epithelium of non-pregnant human endometrium during the menstrual cycle.

Background: Nucleophosmin plays a critical role in embryonic development. This study aimed to examine the expression pattern of nucleophosmin in glandular epithelium of human endometrium during the menstrual cycle.

Methods: Endometrial tissues used for this study were obtained from 46 non-pregnant patients who underwent hysterectomy which had been performed to treat benign diseases.

[The expression of a novel estrogen receptor, GPR30, in epithelial ovarian carcinoma and its correlation with MMP-9].

The aim of the present study is to investigate the expression of a novel estrogen receptor, G protein-coupled receptor 30 (GPR30) and its correlation with matrix metalloproteinases-9 (MMP-9) in epithelial ovarian cancer (EOC). Ovary tissues were obtained from 39 female patients, including 30 cases of EOC and 9 cases of benign ovarian tumor. Four normal ovary tissues were used as control.

Action mechanism of Zuo Gui Yin Decoction's promotion on estradiol production in rats during the peri-menopausal period.

Aim Of The Study: Although Zuo Gui Yin Decoction has long been used in Traditional Chinese Medicine to treat menopausal symptoms, the underlying mechanism(s) by which these effects are induced remains to be defined. The aim of this study was to investigate the action mechanism of Zuo Gui Yin Decoction on estradiol production in the rat ovary during peri-menopause.

Materials And Methods: The peri-menopausal animal model was established by natural aging.

[Effects of Yikunning on the apoptotic rate and expression of caspase-3 in rat ovaries during perimenopausal period].

Objective: To investigate the effects of Yikunning (compound of Chinese traditional Medicine, YKN) on the apoptotic rate and expression of caspase-3 in rat ovaries during perimenopausal period.

Methods: Thirty female Wistar rats during perimenopausal period were selected by unforced aging. Then the rats were divided into 3 groups randomly: YKN group, livial control group and aged control group.

[Effects of yikunning on the expressions of bcl-2 and bax in rat ovaries during perimenopausal period].

Objective: To investigate the effects of Yikunning (YKN, Chinese Traditional Medicine) on the expressions of bcl-2 and bax in rat ovaries during perimenopausal period.

Methods: Thirty female Wistar rats during perimenopausal period were selected by unforced aging. Then the rats were divided into 3 groups randomly: YKN group, Livial control group and Aged control group.

[Effect of zuoguiyin on the expression of ovarian follicle-stimulating hormone receptor in rats during peri-menopausal period].

Objective: To investigate the effect and mechanism of Zuoguiyin (ZGY, a Chinese medical remedy for regulating qi and nourishing yin) on expression of ovarian follicle-stimulating hormone receptor (FSHR) in rats during peri-menopausal period (PMP).

Methods: PMP rats were administered with low (13.78 g/kg), middle (20.

[Changes of granulocyte distribution induced by thyroxin and epinephrine: experiment with rabbits].

Objective: To investigate the distribution of granulocyte during thyrotoxicosis and the relation of granulopenia and the gathering of circulating granulocytes into the marginal pool.

Methods: Forty-five rabbits were randomly divided into 3 equal groups: epinephrine group fed with levo-thyroxin (LT4) for 14 days, injected subcutaneously with epinephrine 0.07 mg/kg, and undergoing collection of peripheral blood samples for white blood cell and granulocyte counts, free blood triiodothyroxine (FT3) and free blood thyroxine (FT4), electrocardiography, and body weight measurement; labeled group fed with LT4 for 14 days, injected subcutaneously with 131I-labeled anti-human granulocyte monoclonal antibody, and killed 6 hours later to calculate the ratio of radioactivity of heart, liver, spleen, and muscle tissues to blood (CPM/g), and control group, not fed with L-T4 but injected with 131I-labeled anti-human granulocyte monoclonal antibody on the day 14, and killed 6 hours later to calculate the ratio of radioactivity of tissues to blood.

[Role of platelet-activating factor in progesterone synthesis and vascular endothelial growth factor expression in rat luteal cells].

The present study aimed to investigate the role of platelet-activating factor (PAF) in progesterone synthesis and vascular endothelial growth factor (VEGF) expression in rat luteal cells. Immature (25-28 days old) female Sprague-Dawley rats were injected subcutaneously with 50 IU pregnant mare serum gonadotrophin (PMSG), and 25 IU human chorionic gonadotrophin (hCG) 48 h later, to induce follicular development and luteum formation. On day 6 after hCG administration (the day of hCG administration was the first day), the rats were killed by guillotine and the ovarian luteal cells were collected.

[Effects of soy isoflavones on the expression of Bax mRNA and Ca(2+)-ATPase activity in ovaries of perimenopause rats].

Aim: To investigate the effects of soy isoflavones (SI) on the expression of Bax mRNA and Ca(2+) -ATPase activity in ovaries of perimenopause rats.

Methods: The animal model of perimenopause rats was established by unforced aging. 12 month-old presenilins female Wistar rats were administered by intragastric (ig) with low (500 mg/kg), middle (158 mg/kg) and high (500 mg/kg) does of SI for 8 weeks.

[Genetic mapping of the intergenomic translocated "taigu" genic sterility gene (Ms2)].

The "Taigu" genic sterility gene Ms2 located on the short arm of the 4D chromosome of common wheat (AABBDD) originally incorporated into hexoploid triticale (AABBRR) and durum wheat (AABB) through intergenomic translocation in distant hybridization was introduced back into the genomes of common wheat. The dominant male sterility was expressed normally in the new "Taigu" genic sterile wheat carrying the intergenomically translocated Ms2, and the female fertility mechanism in its male sterile plants was normal as well. Observation of the chromosome configuration at meiosis of pollen mother cells (PMC) of the young ears of the sterile plants showed that they were euploid plants (2n = 42).