Background: Rice taste is closely associated with endosperm composition, which varies among different rice layers. Although clarifying the relationship between this difference and nutritional taste can guide rice breeding and cultivation practices, research on this topic is limited.
Results: Here, typical rice varieties having excellent and poor taste characteristics were selected to analyze the distribution characteristics and differences of their components.
Repeated silica gel column chromatography, reversed-phase C_(18) column chromatography, Sephadex LH-20 column chromatography, high performance liquid chromatography and semi-preparative medium pressure liquid chromatography were performed to separate and purify the chemical constituents of Hypericum lagarocladum. Spectroscopic methods such as mass spectrometry(MS) and nuclear magnetic resonance(NMR) combined with physicochemical properties were adopted in identifying the structure of the isolated compounds. Ten compounds were isolated from the ethyl acetate fraction of H.
View Article and Find Full Text PDFL. is an annual herbaceous medicinal plant of Zygophyllaceae, which is cultivated commercially in China. Subrotund or irregular gray, sunken, necrotic spots ranging from 2 to 9 mm were observed on diseased leaves of landrace in Fushun County, Liaoning Province of northeast China in July 2021, with more than 32% of the plants being infected in a 18-ha field.
View Article and Find Full Text PDFClarifying the association of differences in endosperm biochemical composition with nutrient and edible quality can guide rice breeding and cultivation. In this study, very-low-amylose-content (LAC), Japanese good-taste (JTC), Northeast China good-taste (CTC), and Northeast China high-yield (CHC) cultivars were milled to obtain four layers (L1-L4) from outside-in, and then analyzed for apparent amylose content (AAC), protein content (PC), amino acids, soluble sugars, and minerals. The association of these compositions and Rapid-Visco analysis values with taste was also examined.
View Article and Find Full Text PDFViscosity, a crucial characteristic for rice palatability, is affected by endosperm characters. We compared correlations between differences in viscosity of japonica rice with various palatability and endosperm characters. Changes in apparent amylose and protein contents (AAC% and PC%, respectively) and amylopectin side-chain distribution and the relationship of these traits with palatability were investigated in superior and inferior spikelets of good cultivars with low amylose content from Hokkaido and common cultivars from northeastern Japan, using rapid visco analyzer characteristics and rice-grain microstructures.
View Article and Find Full Text PDFZhongguo Shi Yan Xue Ye Xue Za Zhi
April 2009
This study was aimed to explore monocyte chemotactic protein 1 (MCP-1) and interleukin 8 (IL-8) expressions in human umbilical vein endothelial cells (HUVECs) affected by TNF-alpha and its molecular mechanism. RT-PCR assay was used to detect the expression of MCP-1 and IL-8 mRNA in HUVECs at various times following stimulation with TNF-alpha; the nuclear factor-kappa B (NF-kappaB) activation in HUVECs were detected by immunofluorescence. The results showed that after stimulation with NF-kappaB, the expression levels of MCP-1 and IL-8 mRNA in HUVECs increased, and changed along with different time; the expression of IL-8 mRNA reached to peak level at 8 hours and the expression of MCP-1 mRNA reached to peak level at 12 hours; the expression of NF-kappaB p65 protein began increasing at 30 minutes and reached to peak level at 1 hour; then the staining of cytoplasm gradually weakened, while staining of nuclei was enhanced, which indicated significant nuclear translocation of NF-kappaB p65.
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