Novel chlorinated and nitrogenated azaphilones with cytotoxic activities from the marine algal-derived fungus 2020HZ23.

Two novel chlorinated and nitrogenated azaphilones, namely -butyl-2-aza-2-deoxychaetoviridin A () and -hexyl-2-aza-2-deoxychaetoviridin A (), along with a previously identified analogue, chaetoviridin A (), were successfully obtained from 2020HZ23, a marine algal-sourced endophytic fungus. The planar structures as well as the absolute configurations of these new metabolites were determined utilizing a synergistic approach that involved both spectroscopic techniques (1D/2D NMR and HRESIMS) and Density Functional Theory (DFT) calculations. Each compound was subject to cytotoxicity evaluation toward the A549 cancer cell line.

A novel magnetic resonance-positive emission image registration based on morphology.

Structures were obtained from images with morphological characteristics to register with voxel-based method. We applied simple morphological operations to obtain human brain cortex and chose normalized mutual information as similarity measure for the geometric alignment of PET and MR images. Evaluation used nine patients, and the results showed that sub-voxel accuracy was achieved and the registration process was significantly more rapid.