Parallel evolution and control method for predicting the effectiveness of non-pharmaceutical interventions in pandemics.

Aim: Nonpharmaceutical interventions (NPIs) are important strategies to utilize in reducing the negative systemic impact pandemic disasters have on human health. However, early on in the pandemic, the lack of prior knowledge and the rapidly changing nature of pandemics make it challenging to construct effective epidemiological models that can be used for anti-contagion decision-making.

Subject And Methods: Based on the parallel control and management theory (PCM) and epidemiological models, we developed a Parallel Evolution and Control Framework for Epidemics (PECFE), which can optimize epidemiological models according to the dynamic information during the evolution of pandemics.

Parallel evolution and response decision method for public sentiment based on system dynamics.

Governments face difficulties in policy making in many areas such as health, food safety, and large-scale projects where public perceptions can be misplaced. For example, the adoption of the MMR vaccine has been opposed due to the publicity indicating an erroneous link between the vaccine and autism. This research proposes the "Parallel Evolution and Response Decision Framework for Public Sentiments" as a real-time decision-making method to simulate and control the public sentiment evolution mechanisms.

Tick-borne agents in rodents, China, 2004-2006.

A total of 705 rodents from 6 provinces and autonomous regions of mainland People's Republic of China were tested by PCRs for tick-borne agents (Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, spotted fever group rickettsiae, and Francisella tularensis). Infection rates were 5.5%, 6.

[Preliminary study on the use of DNA suspension array for the identification of corneal pathogenic fungi].

Objective: To develop a multiplex, microsphere-based DNA suspension array for the identification of important pathogenic fungi of cornea and to study the feasibility of its application in the clinical diagnosis of fungal keratitis.

Methods: Fusarium solani, Fusarium moniliforme, Fusarium oxysporum, Aspergillus fumigatus and Aspergillus flavus, which covered about 80% of pathogenic fungi of fungal keratitis, were chosen as target species of this study. Five species-specific capture probes were designed in the internal transcribed spacer (ITS) regions of ribosomal DNA and synthesized with 5' amino modifier C12 to covalently bind to different sets of fluorescent beads.

MDR1 single nucleotide polymorphism G2677T/A and haplotype are correlated with response to docetaxel-cisplatin chemotherapy in patients with non-small-cell lung cancer.

Background: The multidrug resistance gene 1 (MDR1) encodes P-glycoprotein (P-gp), which plays an important role in mediating multidrug resistance to chemotherapeutic agents. MDR1 gene polymorphisms may have an impact on the expression and function of P-gp, thereby influencing the response to chemotherapy.

Objectives: To investigate whether the MDR1 2677 and 3435 genotypes are associated with the sensitivity of non-small-cell lung cancer (NSCLC) to docetaxel.

MDR1 single nucleotide polymorphisms predict response to vinorelbine-based chemotherapy in patients with non-small cell lung cancer.

Background: The polymorphisms of genes participate in metabolism and transport, and therefore may have an impact on the response to vinorelbine.

Objectives: To investigate whether genotypes of CYP3A5, MDR1 and cyclooxygenase-2 (COX-2) are associated with the response to vinorelbine in non-small cell lung cancers (NSCLC).

Methods: We determined the genotypes of CYP3A5(*3), MDR1 (2677G-->T at exon 21 and 3435C-->T at exon 26 and their haplotypes) and COX-2 (-1195G-->A) polymorphisms by PCR-RFLP and chemotherapy response in 69 Chinese Han patients with NSCLC who received a combination chemotherapy of vinorelbine-cisplatin (VC).

[Investigation on Anaplasma phagocytophilum infection in rodents from forest areas in northeastern China].

Objective: To investigate the prevalence of Anaplasma phagocytophilum in rodents from forest areas in northeastern China.

Methods: PCR amplification, followed by sequence analysis was carried out. The sequences of 16S rRNA and gltA gene fragment amplified from rodent specimens were compared with corresponding part of the sequences deposited in GenBank.

CYP450 polymorphisms predict clinic outcomes to vinorelbine-based chemotherapy in patients with non-small-cell lung cancer.

CYP2C19*2(G681A), CYP2C19*3(G636A), CYP2D6*4(C188T), CYP2D6*2(C2938T, G4268C), CYP3AP1*3- G44A and CYP3A5*3(A22893G) are the most common polymorphisms detected among Chinese that may influence the efficacy of vinorelbine-based therapies to treat non-small-cell lung cancer (NSCLC). We detected the genotypes of these polymorphisms by PCR-RFLP in 59 patients with NSCLC and assessed their responses to vinorelbine. CYP2D6*4(C188T), CYP3AP1*3 (G -44 A) and CYP3A5*3 were found to be associated with response to vinorelbine.

[Study on the coinfection status of Borrelia burgdorferi sensu lato and spotted fever group Rickettsia in ticks from Hunchun, Jilin province].

Objective: To understand the coinfection status of Borrelia burgdorferi sensu lato (B.b.s.

Effects of aspirin on metastasis-associated gene expression detected by cDNA microarray.

Aim: To investigate the effect of aspirin on the metastasis-associated gene expression in 3AO ovarian cancer cells.

Methods: 3AO cells were treated with aspirin at the concentration of 1.2 mmol/L for 16 and 48 h, respectively.

[rpsL gene analysis associated with Streptomycin resistance in Mycobacterium tuberculosis].

The mutation of the rpsL gene in Mycobacterium tuberculosis (M.TB) is related to Streptomycin resistance. In this experiment, 77 TB strains obtained in hospital were selected, the routine drug susceptibility test was done by using BACIEC460 system, meanwhile the TB-rspL gene was amplicated by PCR, and analyzed by SSCP, RFLP and sequence analysis.