[Technology and MethodsSimultaneous Determination of 9 Natural Ingredientsin Functional Food for Anti-hangover and Hepatoprotection by High Performance Liquid Chromatography].

Objectives: To develop a rapid method of high performance liquid chromatography coupled with variable wave length UV detection for simultaneous determination of 9 natural functional ingredients including puerarin, silymarin, quercetin hydrate, schisandrol A, curcumin, tanshinoneI, tanshinoneIIA, cryptotanshinone, and dihydrotanshinoneIin functional food for anti-hangover and hepatoprotection.

Methods: The samples were ultrasonically extracted with 90 % ethanol () and centrifuged at 10 000 r/min for 10 min prior to HPLC analysis. The nine target analytes were separated on a C8 column with gradient elution using methanol and water (The pH value was adjusted to 2.