Objective: To explore the mechanism of bakuchiol on anti-aging gene mRNA expression level of human skin fibroblasts (ESF-1).
Methods: The potential of cell proliferation which was divided into blank group,positive control estradiol group, and bakuchiol high, medium and low dose groups was detected by MTT. The expression levels of Col I, Col III, TIMP-1, TIMP-2 and MMP-1 mRNA were detected with RT-PCR.