Publications by authors named "Hai-Man Liang"
The morphological changes in the cultures of sepal segments in Sinningia speciosa Hiern were observed with Zeiss Stemi 2000-C Stereomicroscope from 0 to 65 days after culture in vitro. The light yellow globular protuberances were observed on the cut edge and the surface of sepal segments after culture for 24 days. Then the globular protuberances grew bigger gradually.
View Article and Find Full Text PDF[Expression of CFL gene during differentiation of floral and vegetative buds in cucumber cotyledonary nodes cultured in vitro].
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao
December 2004
CFL gene, a LFY homologue, was cloned from cucumber (Cucumis sativus L.). In this paper, in situ hybridization was performed to analyze the expression pattern of CFL gene at the stage of floral and vegetative buds differentiation in cucumber cotyledonary nodes cultured in vitro.
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- The paper reviews recent advances in understanding the LFY gene's regulation network and its role in flower development in Arabidopsis thaliana and similar plants.
- The LFY gene is crucial for flower formation, requiring interactions with numerous other genes, and its expression levels significantly influence flowering time and floral structure development.
- Physiological factors, such as carbon sources and hormones, can directly or indirectly affect LFY gene expression, suggesting potential methods for manipulating flowering processes.
Cotyledonary nodes of cucumber cultured on calcium-free medium for 0, 1, 2, 3, 4, 5, 6d respectively, were transferred to medium with 6.0 mmol/L CaCl2 for 24h, then returned to calcium-free medium. Cotyledonary nodes cultured on calcium-free or 6.
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- The study observed that young flower buds and sepals of Sinningia speciosa could regenerate floral buds directly when cultured in vitro.
- A key factor for successful regeneration was the gibberellin concentration; specifically, concentrations above 0.5 mg/L were effective, with optimal results at 1.0-1.5 mg/L GA combined with 0.1 mg/L 6-benzylaminopurine.
- Two types of floral buds were produced: one type consisted solely of floral buds (up to 14.3%) and the other type included both floral and vegetative buds (up to 28.6%).
Changes of endogenous hormones and polyamines(PAs) contents during floral differentiation (0-6 day) in cucumber cotyledonary nodes cultured in vitro were determined by using HPLC. The results showed that all four endogenous hormones decreased obviously within 0-2 day and increased slightly within 4-5 day after culture. This indicated that low levels of indole-3-acetic acid (IAA), gibberelline(GA3) and abscisin acid(ABA) during 0-2 day were favorable to floral primordia differentiation, while high level of zeatin(ZT) during 3-5 day was favorable to floral organ primordia differentiation.
View Article and Find Full Text PDF[Floral homeotic variants of in vitro seedlings in Sinningia speciosa Hiern].
Shi Yan Sheng Wu Xue Bao
February 2003
Six types of floral homeotic variants of in vitro seedlings were observed in doubleflower sinningia. Type I, red and green mosaic petals exist in the outermost whorl of petal-whorls, 2.38%.
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