Fabrication of 3D-Printed Interpenetrating Hydrogel Scaffolds for Promoting Chondrogenic Differentiation.

The limited self-healing ability of cartilage necessitates the application of alternative tissue engineering strategies for repairing the damaged tissue and restoring its normal function. Compared to conventional tissue engineering strategies, three-dimensional (3D) printing offers a greater potential for developing tissue-engineered scaffolds. Herein, we prepared a novel photocrosslinked printable cartilage ink comprising of polyethylene glycol diacrylate (PEGDA), gelatin methacryloyl (GelMA), and chondroitin sulfate methacrylate (CSMA).

Vortioxetine induces apoptosis and autophagy of gastric cancer AGS cells via the PI3K/AKT pathway.

Vortioxetine is a potent antagonist of the 5-hydroxytryptamine receptor and serotonin transporter and has been reported to function as an antidepressant in the treatment of major depressive disorder. However, its antitumor effects remain unclear. Here, we examined whether vortioxetine affects the characteristics of GC cells.

[Treatment Status and Emission Characteristics of Volatile Organic Compounds from Typical Industrial Sources].

The status of treatment equipment, the emission characteristics, and the ozone formation potential (OFP) of volatile organic compounds (VOCs) for 11 typical enterprises, which were categorized into the 8 major VOC emission industries identified by the emission inventory of a typical city in the Yangtze River Delta, are discussed in this paper. There was a large difference in the removal efficiency of non-methane hydrocarbon (NMHC) between different treatment techniques, and even an increase in concentration occurred after some of the treatments. The current treatment equipment for VOCs needs further optimization.

[Cultivation and purification of high proliferative potential endothelial progenitor cells from murine bone marrow].

The present study was designed to culture and purify high proliferative potential endothelial progenitor cells (HPP-EPCs) from murine bone marrow and their progeny cells using a culture system which has been established by us recently, in order for more intensive researches on these cells. Fresh murine bone marrow cells were preplated to allow the preferential attachment of non-EPCs to tissue culture plates and then unattached cells were repreplated in the culture system containing the bone marrow endothelial cell line-conditioned medium (BMEC-CM). The colonies containing about 2 x 10(4) cells were collected respectively and single colony-derived cells were cultured for their expansion in the above-mentioned culture system.

Resonance Raman study of the A-band short-time photodissociation dynamics of 2,5-di-iodothiophene.

Resonance Raman spectra were obtained for 2,5-di-iodothiophene in cyclohexane solution with excitation wavelengths in resonance with its A-band absorption. These spectra indicate that the Franck-Condon region photodissociation dynamics have multidimensional character with motion mostly along the nominal symmetric C(2)C(3)C(4)C(5) stretch nu(2) (1388 cm(-1)) of the thienyl ring and this is accompanied by motions along the nominal symmetric CSC stretch nu(6) (640 cm(-1)), the nominal symmetric C[Single Bond]I stretch nu(7) (187 cm(-1)), the nominal antisymmetric CSC stretch nu(19) (728 cm(-1)), the nominal antisymmetric C(2)C(3)C(4)C(5) stretch nu(16) (1497 cm(-1)), the nominal C(3)C(4) stretch nu(3) (1286 cm(-1)) and four other vibrational modes. A preliminary resonance Raman intensity analysis was done and the results for 2,5-di-iodothiophene were compared to previous results for 2-iodothiophene and di-iodomethane to discuss the mechanism of the symmetric breaking in the initial A-band photodissociation dynamics of 2,5-di-iodothiophene and 2-iodothiophene.

[Dose-effect relationship of DMSO and Tween 80 influencing the growth and viability of murine bone marrow-derived cells in vitro].

This study was purpose to examine the effect of dimethyl sulfoxide (DMSO) and Tween 80 on the growth and viability of stromal cells (BMSC), colony-forming units for granulocytes and macrophages (CFU-GM) and bone marrow endothelial cell line (BMEC) from murine bone marrow in vitro, and to analyze the concentration-effect relationship. The colony yields of colony-forming units fibroblastic (CFU-F) and CFU-GM were assessed in the murine bone marrow cell cultures at various concentrations of DMSO or Tween 80 and in the control groups. The MTT assay and trypan blue exclusion were used to determine the cell viability and percentage of survival in BMSC and BMEC cultures with or without either of these organic solvents.

Resonance Raman study of the A-band short-time photodissociation dynamics of 2-iodothiophene.

Resonance Raman spectra were obtained for 2-iodothiophene in cyclohexane solution with excitation wavelengths in resonance with the A-band absorption spectrum. These resonance Raman spectra indicate that the Franck-Condon region photodissociation dynamics have multidimensional character with motion mainly along the nominal symmetric C=C stretch of the thienyl ring and accompanied by a moderate amount of motion along the nominal symmetric CSC stretch, the nominal antisymmetric CSC stretch, and the nominal C-I stretch vibrational modes. A preliminary resonance Raman intensity analysis was done for the A-band resonance Raman spectra of 2-iodothiophene.