2D Cerium-Organic Frameworks as an Efficient Heterogeneous Catalyst for the Synthesis of 1,4-Dihydropyridines via Hantzsch Reaction.

Herein, a new two-dimensional (2D) Ce-organic frameworks (referred to as SLX-4) was achieved by traditional solvothermal conditions. Initial studies of SLX-4 toward Hantzsch reaction showed that good catalytic activity can be obtained under mild conditions, giving the desired 1,4-dihydropyridines in moderate to high yields. The catalyst could be reused at least 4 times keeping good catalytic activity.

Enterprise stents versus low-profile visualized intraluminal support stents for stent-assisted coiling of unruptured paraclinoid aneurysms.

Background: The microsurgical treatment of paraclinoid aneurysms can be challenging due to the anatomical structures that surround them.

Objective: This study compared the clinical and angiographic outcomes of unruptured paraclinoid aneurysms treated with enterprise (EP) stents and low-profile visualized intraluminal support (LVIS) stents.

Methods: A retrospective analysis of the clinical and radiological data from 133 patients with 139 unruptured paraclinoid aneurysms, who received an EP or an LVIS stent between January 2017 and June 2021 at Taizhou People's Hospital, was performed.

Dysregulation of GTPase IMAP family members in hepatocellular cancer.

Hepatocellular carcinoma (HCC) is one of the most life‑threatening diseases in the world. Members of the GTPase of the immunity‑associated protein (GIMAP) family are important in regulating apoptosis in cancer cells. However, the basic mechanism of GIMAP in HCC remains to be fully elucidated.

[Effect of Danshen-containing serum on expression of SuFu and DYRK2 in HSCs].

To observe the effects of Danshen-containing serum on SuFu and DYRK2 expression in the HSCs stimulated by leptin. SD rats (n = 60) were used to make danshen-containing serum by gastric perfusion for ten days with Danshen water decoction, normal saline and colchicine. The HSCs that were cultured in vitro would be stimulated for 24 hours by leptin (100 μg x L(-1)) except blank control group, after being intervened, the drug serum in each group would be cultured at 37 degrees C in 5% incubator.

[Effect of Ligusticum wallichii-containing serum on expressions of Toll-like receptor 4 and myeloid differentiation factor 88 in hepatic stellate cells].

To observe the effect of Ligusticum wallichii-containing serum on the expressions of Toll-like receptor 4 and myeloid differentiation factor 88 in hepatic stellate cells. Clean-grade SD rats were randomly divided into 5 groups and orally given L. wallichii decoction, colchicine and normal saline for 7 d to prepare L.

Voluntary exercise prevents colonic inflammation in high-fat diet-induced obese mice by up-regulating PPAR-γ activity.

Obesity is associated with increased colonic inflammation, which elevates the risk of colon cancer. Although exercise exerts anti-inflammatory actions in multiple chronic diseases associated with inflammation, it is unknown whether this strategy prevents colonic inflammation in obesity. We hypothesized that voluntary exercise would suppress colonic inflammation in high-fat diet (HFD)-induced obesity by modulation of peroxisome proliferator-activated receptor (PPAR)-γ.

[Studying the changes of the related serum complement immune indexes in patients with occupational medicamentosa-like dermatitis induced by trichloroethylene and workers occupationally exposed to trichloroethylene].

Objective: To explore the mechanism of occupational medicamentosa-like dermatitis (OMDT) induced by trichloroethylene (TCE) and some immunity indexes in workers occupationally exposed to TCE.

Methods: The blood samples from 8 cases with medicamentosa-like dermatitis in 1st, 2nd, 3rd, 4th and 5th weeks after admitting to hospital were examined for liver function, immunoglobulin and some complement indexes. Thirty nine workers occupationally exposed to TCE were investigated for urinary TCE and some immuno-complement indexes.

[Isolation, purification and biological activities of Arnebia euchroma glycosaminoglycans].

Objective: To study the methods of extraction, isolation, purification and biological activities of Arnebia euchroma glycosaminoglycans (AEG).

Methods: AEG was purified by distilled water extraction, ethanol fractionation, Sephadex column chromatography. The purity and molecular weight and concentration of AEG were measured by HPLC; We divided the experiment into Physiological Saline group and the other eight groups with different doses of AEG, established RT-PRC method to observe the anti-HPV effect after their actions on the verruca tissues.

[PDX-1 gene transfection and expression in bone marrow stromal cells-derived nestin-positive cells].

Objective: To introduce the eukaryotic expression vector pEGFP-C1-PDX-1 into nestin-positive cell derived from bone marrow stromal cells by nucleofection and optimize the conditions for transfection.

Methods: The recombinant plasmid was transfected into bone marrow stromal cells-derived nestin-positive cells with varied DNA quantities or the serum concentration in the medium. The expression of PDX-1 gene in the transfected cells was detected by RT-PCR.

[Induced differentiation of bone marrow stem cells in transplanted rat liver].

Objective: To investigate the differentiation of bone marrow stem cells in transplanted livers and its impact on the long-term survival of rats with orthotopic liver transplantation.

Methods: Twenty-four female recipient rats with orthotopic liver transplantation were randomized into blank-control group, D-hanks solution group, bone marrow stem cells group with postoperative infusion of stem cells, and the pathological changes of the liver grafts and survival time of the rats were observed. The differentiation of the bone marrow stem cells were assessed 60 days after transplantation using in situ hybridization histochemistry for Sry gene and alpha-fetoprotein (AFP) immunohistochemistry.

[Protective effect of donator liver ischemic preconditioning on posttransplant liver graft function in Chinese miniature pigs].

Objective: To investigate the protective effect of the ischemia preconditioning of the donor liver on posttransplant liver graft function in Chinese miniature pigs.

Methods: Twenty-five partially inbred Chinese miniature pigs were randomized into three groups, namely the normal control group, the ischemia-reperfusion group and ischemic preconditioning group. Biopsies of the liver graft were performed to analyze HSP70 expression by means of immunoblotting, and the changes of serum AST/ALT levels were assayed using an automated biochemical analyzer.