[Predictive value of fluorescence in situ hybridization in patients with bladder cancer].

Objective: To assess the value of fluorescence in situ hybridization (FISH) in the diagnosis of bladder cancer.

Methods: Urine samples from 100 patients suspected of having bladder cancer were collected before cystoscopy for immediate urine cytology and FISH analysis. The criteria for FISH abnormality were determined by evaluating the urine specimens from 20 subjects without urogenital neoplasm.

[Association of S100A4 mRNA expression with differentiation and metastasis of renal cell carcinoma].

Objective: To study the association of S100A4 mRNA expression level with the differentiation and metastasis of renal cell carcinoma (RCC).

Methods: Tissue specimens were obtained from 31 patients undergoing surgery for renal cell carcinomas between May 2001 and May 2004. Reverse transcriptional PCR was performed for amplification of S100A4 mRNA (212 bp) from 31 RCC tissues and matched normal kidney tissues besides the tumor, and the differential S100A4 mRNA expression was analyzed for its association with the clinical manifestations of the patients.

[Clinical effects of preoperative chemotherapy for Wilms tumor].

Objective: To evaluate the effects of preoperative chemotherapy for Wilms' tumor.

Methods: Thirty-five surgically treated cases of Wilms tumor were retrospectively analyzed, 27 of which received preoperative chemotherapy.

Results: After chemotherapy, the tumor volume reduced obviously in 17 cases and adverse effects of the chemotherapeutic agents occurred in 15 cases.

[Selection of the imaging modalities for diagnosis of renal trauma: experience with 74 cases].

Objective: To explore the diagnostic approaches and values of the imaging modalities for traumatic renal injuries.

Methods: The clinical records of 74 cases of renal trauma treated in Nanfang Hospital were retrospectively reviewed to assess the diagnostic value of intravenous urography (IVU), type B ultrasonography and computed tomography (CT).

Results: The positivity rates by IVU, type B ultrasonography, and CT were 89% (43/49), 80% (55/68) and 100% (51/51) respectively for the diagnosis of renal trauma.

[Cyclosporin and portal venous inoculation of donor splenocytes prolongs cardiac allograft survival in mice].

Objective: To investigate the effect of portal venous inoculation of donor splenocytes combined with cyclosporin A (CsA) administration on cardiac allograft survival in mice.

Methods: Heterotopic cardiac transplantation between fully allogenic NIH/q and BALB/C strain mice was performed. A modified procedure of neonatal heart-in-ear transplantation, as originally described by Fulmer et al, was adopted.

Fertilizing capacity of human sperm preserved in cold electrolyte-free solution.

Objective: To assess the fertilizing capacity of human sperm preserved in electrolyte-free (EF) solution at 4 degrees Celsius.

Methods: The motility, acrosome status and fertility index of human sperm were respectively assessed before and after preservation in cold EF solution.

Results: The motility of human sperm so preserved for one week was significantly higher than that of the sperm preserved in modified human tubal fluid (43.