Modification of C-segment of Cfa DnaE split intein for improving clean-in-place in chromatography process.

This research focuses on the construction of an affinity purification system based on Cfa DnaE split intein. Cfa DnaE intein is an artificially constructed intein with the advantages of a fast cleavage reaction and good stability. In a previous study, a purification system that uses Cfa intein as a tag was constructed, the separation of the target protein and the tag during the purification process was completed, and the purity of the purified target protein reached 98.

Flexible FeVO porous nanorods on carbon cloth for long-life aqueous energy storage.

We report the FeVO porous nanorods on carbon cloth as a novel cathode material for flexible aqueous energy storage. It exhibits excellent electrochemical properties and cycling stability in supercapacitors and zinc-ion batteries. Moreover, this work makes significant progress for developing high-performance electrodes and provides a foundation for future research.

Improved protein purification system based on C-terminal cleavage of Npu DnaE split intein.

A purification system was constructed with the N-segment of the Npu DnaE split intein as an affinity ligand immobilized onto an epoxy-activated medium and the C-segment used as the cleavable tag fusing target protein. The affinity properties of C-tagged proteins adsorbed on I affinity chromatography medium were studied with GFP as a model target protein. The saturated adsorption capacity and dynamic adsorption capacity reached 51.

Corallincola holothuriorum sp. nov., a facultative anaerobe isolated from sea cucumber intestine.

Strain C4, isolated from sea cucumber intestine in Weihai, Shandong, PR China, is a novel Gram-stain-negative, facultatively anaerobic, amphitrichously flagellated, short rod that grows as creamy white bacterial colonies on plates. Optimal growth of the strain was observed at 28-30 °C, pH 6.5-7.

Bizionia sediminis sp. nov., isolated from coastal sediment.

A Gram-stain-negative, aerobic, non-gliding, rod-shaped and orange-coloured bacterium, designated strain P131T, was isolated from marine sediment of the coast of Weihai, China, and subjected to a polyphasic study. Strain P131T was found to grow optimally at 28-30 °C, at pH 7.0-7.

Aquimarina rubra sp. nov., isolated from sediment of a sea cucumber culture pond.

A Gram-stain-negative, non-motile, rod-shaped, red-pigmented, facultatively anaerobic bacterium, designated SS2-9T, was isolated from sediment collected from a sea cucumber culture pond located in Rongcheng, Shandong province, China. Cells of strain SS2-9T were approximately 0.3-0.

Mixed-mode chromatography integrated with high-performance liquid chromatography for protein analysis and separation: Using bovine serum albumin and lysozyme as the model target.

A type of mixed-mode chromatography was integrated with high-performance liquid chromatography for protein analysis and separation. The chromatographic behavior was tested using bovine serum albumin and lysozyme as model proteins. For the mixed-mode column, the silica beads were activated with γ-(2,3-epoxypropoxy)-propytrimethoxysilane and coupled with 4-mercaptopyridine as the functional ligand.

Molecular modification of Protein A to improve the elution pH and alkali resistance in affinity chromatography.

Protein A of Staphylococcus aureus has been widely used as an affinity ligand for the purification of immunoglobulin. However, the low elution pH and the sensitivity to alkaline condition restricted the large-scale application of antibody purification. To overcome these disadvantages, the B domain was selected and mutated to Z domain and the recombinant Protein A was reconstructed by linking five Z domains.

Salegentibacter echinorum sp. nov., isolated from the sea urchin Hemicentrotus pulcherrimus.

A novel Gram-negative, strictly aerobic, heterotrophic, non-motile and yellow-pigmented bacterial strain, designated HD4(T), was isolated from the sea urchin Hemicentrotus pulcherrimus collected from the Yellow Sea in China. Optimal growth of the strain was observed at 28-30 °C, pH 6.8-7.

[Cloning and functional analysis of SCTF-1 encoding a C2H2-type Zinc finger protein from soybean].

The zinc finger protein is one of the proteins with finger-like domain. Some of them are transcription factors which play important role in plant growth and plant resistance to abiotic stresses. In this paper, a novel C2H2-type zinc finger protein gene SCTF-1 (GenBank accession number JQ692081) was isolated from soybean (Glycine max (L.

Chromatographic performance of macroporous cellulose-tungsten carbide composite beads as anion-exchanger for expanded bed adsorption at high fluid velocity.

Macroporous cellulose-tungsten carbide composite beads was designed and prepared as an anion-exchanger for expanded bed adsorption (EBA). The wet density of composite beads was adjusted at the range of 1.2-2.

Preparation and characterization of macroporous cellulose-tungsten carbide composite beads for expanded bed applications.

The specially designed adsorbent is one of the most important bases to achieve the expanded bed adsorption (EBA) process. The perfect adsorbents tend to be of small size, large pore and high density. In the present work a new kind of macroporous cellulose beads densified by high-density tungsten carbide were prepared through the method of water-in-oil suspension thermal regeneration.

Evaluation of new high-density ion exchange adsorbents for expanded bed adsorption chromatography.

New adsorbents Q HyperZ and CM HyperZ composed of hydrogel-filled porous zirconium oxide particles were evaluated for expanded bed adsorption applications in the present work. The HyperZ adsorbents have wet density of 3.16 g ml(-1), particle size of 44.