[Cloning and functional characterization of two oxidosqualene cyclase genes from Siraitia grosvenorii].

Oxidosqualene cyclases(OSCs), belonging to a multigene family, can convert a common precursor 2,3-oxidosqualene into various types of triterpene skeletons. In this study, primers were designed according to the analysis of Siraitia grosvenorii transcriptome data, and two OSC genes SgAS1(GenBank No. QDO67189.

Identification of a cytochrome P450 from Tripterygium hypoglaucum (Levl.) Hutch that catalyzes polpunonic acid formation in celastrol biosynthesis.

Tripterygium hypoglaucum (Levl.) Hutch, a traditional Chinese medicinal herb with a long history of use, is widely distributed in China. One of its main active components, celastrol, has great potential to be developed into anti-cancer and anti-obesity drugs.

Metabolic Engineering of for High-Level Friedelin Genetic Manipulation.

Friedelin, the most rearranged pentacyclic triterpene, also exhibits remarkable pharmacological and anti-insect activities. In particular, celastrol with friedelin as the skeleton, which is derived from the medicinal plant , is a promising drug due to its anticancer and antiobesity activities. Although a previous study achieved friedelin production using engineered , strains capable of producing high-level friedelin have not been stably engineered.

Optimized splitting of mixed-species RNA sequencing data.

Gene expression studies using xenograft transplants or co-culture systems, usually with mixed human and mouse cells, have proven to be valuable to uncover cellular dynamics during development or in disease models. However, the mRNA sequence similarities among species presents a challenge for accurate transcript quantification. To identify optimal strategies for analyzing mixed-species RNA sequencing data, we evaluate both alignment-dependent and alignment-independent methods.

Clinical effect of Changweishu on gastrointestinal dysfunction in patients with sepsis.

Objective: To investigate Changweishu's clinical effect on gastrointestinal dysfunction in patients with sepsis.

Methods: Fifty patients with gastrointestinal dysfunction and sepsis were randomly divided into treatment and control groups. The control group patients received routine Western medicine treatments (meropenem, noradrenaline, glutamine glue, triple-strain tablet), and the treatment group patients received routine Western medicine treatment combined with Changweishu.