Inulin-type fructans obtained from Atractylodis Macrocephalae by water/alkali extraction and immunoregulatory evaluation.

Two homogenous polysaccharides extracted from Atractylodes macrocephala Koidz. were investigated by water extraction (AMP-F) and alkali solution extraction (AMP-F) after purification by anion exchange column and size exclusion chromatography. The molecular weight of AMP-F and AMP-F were 2874 Da and 3438 Da, respectively, estimated by high performance gel permeation chromatography (HPGPC).

Expression and prognosis analysis of PAQR5 in kidney cancer.

Progestin and adipoQ receptor 5 (PAQR5) affects the development of various malignancies and is specifically expressed in kidney. However, the role of PAQR5 in renal carcinoma remains unclear. We assessed the state of PAQR5 expression in kidney renal clear cell carcinoma (KIRC) by The Cancer Genome Atlas and Gene Expression Omnibus datasets.

Phase I study of pegylated liposomal doxorubicin and cisplatin in patients with advanced osteosarcoma.

Purpose: The repeated use of doxorubicin is limited due to dose-limiting cardiac toxicity. Pegylated liposomal doxorubicin (PEG-LD, Duomeisu) has a reduced cardiac toxicity. This phase I study aimed to investigate the maximum tolerated doses (MTDs) and dose-limiting toxicities (DLTs) of the PEG-LD and cisplatin combination in patients with metastatic and recurrent osteosarcoma.

Simultaneous Preparation of Abundant Flavonol Triglycosides from Tea Leaves.

Flavonol glycosides are important components of tea leaves, contributing to the bioactivities as well as bitterness and astringency of tea. However, the standards of many flavonol triglycosides are still not available, which restricts both sensory and bioactivity studies on flavonol glycosides. In the present study, we established a simultaneous preparation method of seven flavonol triglycoside individuals from tea leaves, which consisted of two steps: polyamide column enrichment and preparative HPLC isolation.

Bursopentin (BP5) induces G1 phase cell cycle arrest and endoplasmic reticulum stress/mitochondria-mediated caspase-dependent apoptosis in human colon cancer HCT116 cells.

Background: Bursopentin (BP5) is a multifunctional pentapeptide found in the chicken bursa of Fabricius. Recent study indicated that BP5 significantly stimulates expression of p53 protein in colon cancer HCT116 cells. However, the effects and underlying mechanisms of BP5 on HCT116 cell proliferation remain largely unclear.

Overexpression of MTHFD1 in hepatocellular carcinoma predicts poorer survival and recurrence.

MTHFD1 was the enzyme providing one-carbon derivatives of tetrahydrofolate. We sought to investigate the impact of MTHFD1 on hepatocellular carcinoma (HCC). Bioinformatic analysis, western blot and immunohistochemistry were conducted to detect MTHFD1 expression in HCC.

lncRNA PCAT6 promotes non-small cell lung cancer cell proliferation, migration and invasion through regulating miR-330-5p.

Background: Investigating the roles of lncRNA prostate cancer-associated transcript 6 (PCAT6) in modulating the growth and aggressiveness of non-small-cell lung carcinoma (NSCLC) cell.

Method: The levels of PCAT6 in NSCLC tissues and cell lines were determined by quantitative real-time PCR assay. MTT as well as colony formation assays were applied to explore the effect of PCAT6 on the growth of NSCLC cell in vitro.

Magnetic Resonance Imaging for the Assessment of Long Bone Tumors.

Background: Wide resection margins of osseous tumors are associated with a low incidence of local recurrence, making accurate measurement of the intraosseous extent of primary malignant long bone tumors is crucial. We compared the intraosseous tumor extent assessed by magnetic resonance imaging (MRI) with the gross specimen to evaluate the accuracy of MRI.

Methods: A total of 255 patients with primary malignant tumors in the long bones were included.

Is There a Reliable Method to Predict the Limb Length Discrepancy after Chemotherapy and Limb Salvage Surgery in Children with Osteosarcoma?

Background: For a child with osteosarcoma, prediction of the limb length discrepancy at maturity is important when planning for limb salvage surgery. The purpose of this study was to provide a reliable prediction method.

Methods: A retrospective review of Chinese children receiving chemotherapy for osteosarcoma before skeletal maturity was conducted.

Computer Navigation-aided Resection of Sacral Chordomas.

Background: Resection of sacral chordomas is challenging. The anatomy is complex, and there are often no bony landmarks to guide the resection. Achieving adequate surgical margins is, therefore, difficult, and the recurrence rate is high.

Subchondral bone grafting reduces degenerative change of knee joint in patients of giant cell tumor of bone.

Background: Giant cell tumors (GCTs) most commonly occur around the knee. The most beneficial procedure for this disease has been extensive curettage with reconstruction. However, since many GCTs may compromise the subchondral bone, surgery can further jeopardize the articular cartilage and result in secondary osteoarthritis.

[Inhibitory effect of 5-fluorouracil on HT-1080 human fibrosarcoma cells in vitro].

Objective: To evaluate the inhibitory effect of 5-fluorouracil (5-Fu) on HT-1080 human fibrosarcoma cells in vitro.

Methods: HT-1080 human fibrosarcoma cells were cultured for 3 days in the proliferation period. Then adriamycin or 5-Fu at different concentrations were used to treat these cells for 24 h, 72 h and 144 h.

Volatile profile analysis and quality prediction of Longjing tea (Camellia sinensis) by HS-SPME/GC-MS.

This study aimed to analyze the volatile chemical profile of Longjing tea, and further develop a prediction model for aroma quality of Longjing tea based on potent odorants. A total of 21 Longjing samples were analyzed by headspace solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). Pearson's linear correlation analysis and partial least square (PLS) regression were applied to investigate the relationship between sensory aroma scores and the volatile compounds.

[Observation on therapeutic effect of acupuncture of Back-shu acupoints for chronic fatigue syndrome patients].

Objective: To observe the therapeutic effect and safety of acupuncture of Back-shu points [Xinshu (BL 15), Pishu (BL 20), etc.] in the treatment of chronic fatigue syndrome (CFS).

Methods: A total of 120 CFS patients were equally randomized Into acupuncture and control groups.

[Retrospective analysis and prognostic factors of 208 cases of primary soft tissue sarcoma of extremity].

Objective: To investigate the prognosis factors of soft tissue sarcoma, especially the impact of surgical treatment on the prognosis.

Methods: We retrospectively reviewed 208 surgically treated patients. There were 128 male and 80 female.

Clinical observations on 46 cases of globus hystericus treated with modified Banxia Houpu decoction.

Objective: To observe the therapeutic effect and mechanism of modified Banxia Houpu decoction on globus hystericus.

Methods: The 95 patients with globus hystericus were randomly divided into a treatment group of 46 cases treated with modified Banxia Houpu decoction and a control group of 49 cases treated with Manyanshuning (Granula for Clearing the Throat). In addition, a normal group of 24 healthy people was set up.

[Surgical treatment for bone nonunion after massive allograft transplantation].

Objective: To evaluate the surgical treatment and outcome of autogenous bone grafting and internal fixation in management of bone nonunion after massive allograft transplantation.

Methods: From January 1994 to December 2006, 41 of 176 patients underwent bone nonunion after massive allograft transplantation. Twenty-two of 41 patients received autogenous bone grafting.

[The status and prospect of total body irradiation technique].

This paper describes the development of TBI technique about fractionated TBI and the relationship between irradiation dose rate and complication; IMRT for TBI and lung compensation technique.

[Design of a 3D afterloading brachytherapy simulation system based on CT images].

Objective: To design a new afterloading brachytherapy simulation system based on CT images.

Methods: This paper mainly focuses on the anthropomorphic pelvic phantom spiled by three pipelines and the nasopharyngeal carcinoma spiled by two pipelines. Microsoft Visual C++ was used to parse CT images for some information, then to reconstruct pipelines in the body of phantom or the patient and to give the three-dimensional coordinate of dwelling points.

[Photosynthesis and active-oxygen-scavenging enzyme activities in rice varieties with different phosphorus efficiency under phosphorus stress].

Using six rice varieties with different phosphorus (P) use efficiency as the materials, the photosynthesis and active-oxygen-scavenging enzyme activities of the rice varieties under different P conditions were studied. Under deficient P condition, the photosynthetic rate (Pn) and soluble protein content (pro) were all gradually decreased with the growth process in the six tested varieties. The Pn and pro were always lower under deficient P condition than those under sufficient P condition.

The effects and mechanisms of primiparity on the risk of pre-eclampsia: a systematic review.

Pre-eclampsia has been dubbed as 'a disease of primiparity'. However, the effects and mechanisms of the association of primiparity with pre-eclampsia have not been clearly defined. We conducted a systematic review of studies evaluating the effect of primiparity on the risk of pre-eclampsia, and studies (published between January 1966 and July 2005) on the mechanisms underlying such an association.