Tissue plasminogen activator in trabecular meshwork attenuates steroid induced outflow resistance in mice.

Tissue plasminogen activator, a serine protease encoded by the PLAT gene is present in the trabecular meshwork (TM) and other ocular tissues and has been reported to be downregulated by treatment with steroids in vitro. Steroids are known to cause changes in outflow facility of aqueous humor in many species. In the present study, we tested whether overexpression of PLAT can prevent and/or reverse the outflow facility of mouse eyes treated with steroids.

Triamcinolone acetonide decreases outflow facility in C57BL/6 mouse eyes.

Purpose: To determine the effect of triamcinolone acetonide (TA) on outflow facility in mice.

Methods: Animals received 20 μL of TA (40 mg/mL) suspension subconjunctivally either bilaterally or unilaterally and were euthanized after either 1 week or 3 weeks. Before mice were killed, IOP was measured with a rebound tonometer.