Causal association between allergic diseases and celiac disease: a bidirectional two-sample and multivariable Mendelian-randomization study.

Objective: This study aimed to assess the causality of allergic diseases and celiac disease.

Methods: We collected summary-level data from publicly available genome-wide association studies to conduct our bidirectional two-sample and multivariable Mendelian randomization analysis. Furthermore, a series of sensitivity analyses were applied to validate our findings.

[A Family with Congenital Dysfibrinogenemia and Blood Transfusion].

Objective: To investigate a family with congenital dysfibrinogenemia, and analyze the risk of hemorrhage and thrombosis and blood transfusion strategies.

Methods: Prothrombin time (PT), activated partial thromboplastin time (APTT) and thrombin time (TT) of the proband and her family members were detected by automatic coagulometer, fibrinogen (Fg) activity and antigen were detected by Clauss method and PT algorithm respectively. Meanwhile, thromboelastometry was analyzed for proband and her family members.

Identification, Screening and Antibacterial Mechanism Analysis of Novel Antimicrobial Peptides from Sturgeon () Spermary.

Fish is an important source of antimicrobial peptides. This study aimed to identify and screen antibacterial peptides with excellent antibacterial activity derived from sturgeon spermary peptides (SSPs) and to analyze their antibacterial activity and mechanism. Liquid chromatography-mass spectrometry/mass spectrometry methods were used to analyze and identify peptide sequences, computational prediction tool and molecular docking methods were used for virtual screening of antimicrobial peptides, and finally, candidate peptides were synthesized by solid-phase synthesis method.

Silver Carp () Scale Collagen Peptides-1 (SCPs1) Inhibit Melanogenesis through Downregulation of the cAMP-CREB Signaling Pathway.

The mechanism of silver carp scale collagen peptides (SCPs1) on melanogenesis and its mechanism of action were examined in mouse melanoma cells (B16). The cell viability and effects of SCPs1 on intracellular tyrosinase (TYR) activity and melanin, reactive oxygen species (ROS), glutathione (GSH) and cyclic adenosine monophosphate (cAMP) content were examined. The regulatory mechanism of SCPs1 on the cAMP response element-binding protein (CREB) signaling pathway was analyzed.

Silver Carp () Scales Collagen Peptides (SCPs): Preparation, Whitening Activity Screening and Characterization.

This study involves the preparation of scale collagen peptides (SCPs) with whitening activity from silver carp () and their characterization and peptide sequence identification. In this article, scanning electron microscopy (SEM) was used to observe structure changes of sliver carp scales; enzymatic hydrolysis was optimized through protease screening and response surface optimization. The ultrafiltration was used to separate SCPs and the whitening activity was comprehensively evaluated using radical scavenging rate and tyrosinase-inhibiting activity, among others.

Antimicrobial activities and mechanism of sturgeon spermary protein extracts against .

This study aimed to evaluate the antimicrobial activities and mechanism of sturgeon spermary protein extracts (SSPE) against . The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. Cell structural change was analyzed using scanning electron microscopy-energy dispersive X-ray spectrometry and transmission electron microscope.

Analysis of Water Distribution and Muscle Quality of Silver Carp () Chunks Based on Electron-Beam Irradiation.

Electron-beam irradiation (EBI) is an efficient, safe, and nonthermal sterilization technique that is extensively used in food preservation research. Here we report the effects of different EBI doses (0, 4, 8 kGy) and preservation temperatures (room temperature [RT], 4 °C) on the muscle water distribution and muscle quality indices of silver carp chunks (SCCs). The highest entrapped water content was found in the 4-kGy-irradiated/4-°C-stored samples.

[Gene Sequencing Analyses of 10 ABO Ambiguous Blood Group Samples].

Objective: Through analysis of ABO blood group gene typing technology, to assist in the identification of difficult clinical serological specimens.

Methods: A total of 10 forwardreverse typing ambiguous samples were collected from January 2021 to August 2021 in our hospital.ABO genotypes were analysed by gene sequencing.

Physicochemical Properties and Biological Activities of Silver Carp Scale Peptide and Its Nanofiltration Fractions.

To explore the physicochemical properties and biological functions of silver carp scale peptide (SCSP), its molecular-weight fractions SCSP-I, II, and III obtained by nanofiltration were assessed for their solubility, emulsibility, free radical scavenging ability, effect on the proliferation of mouse B16 cells. The results showed that the solubility of each fraction of SCSP was higher than 90%, SCSP-II and III were higher than 95%. The antioxidant powers on ⦁OH, ⦁ and Fe were ranked as SCSP-III > SCSP-II > SCSP-I > SCSP.

Cobalt-60 and electron beam irradiation-induced lipid oxidation in largemouth bass (Micropterus salmoides).

Background: Irradiation can cause lipid oxidation of fish. This study aimed to examine the effect of radiation (method, dose and dose rate) on the acid value (AV), peroxide value (PV), thiobarbituric acid reactive substances (TBARS) content and fatty acid profile of fresh and freeze-dried largemouth bass flesh.

Results: AV, PV and TBARS presented a dose-dependent increase in fish meat for both cobalt-60 ( Co) and electron beam (EB) irradiation.

Corosolic Acid Attenuates Hepatic Lipid Accumulation and Inflammatory Response via AMPK/SREBPs and NF-B/MAPK Signaling Pathways.

Corosolic acid (CA) is the main active component of and has been known to serve as several different pharmacological effects, such as antidiabetic, anti-oxidant, and anticancer effects. In this study, effects of CA on the hepatic lipid accumulation were examined using HepG2 cells and tyloxapol (TY)-induced hyperlipidemia ICR mice. CA significantly inhibited hepatic lipid accumulation via inhibition of SREBPs, and its target genes FAS, SCD1, and HMGCR transcription in HepG2 cells.

Imaging findings of inflammatory pseudotumor-like follicular dendritic cell tumors of the liver: Two case reports and literature review.

Background: Inflammatory pseudotumor-like follicular dendritic cell (IPT-like FDC) tumors of the liver is an uncommon tumor with extremely low incidence. To date, the radiologic findings of this tumor in multiphase computed tomography (CT) and magnetic resonance imaging (MRI) imaging have not been described.

Case Summary: Patient 1 is a 31-year-old Chinese female, whose complaining incidentally coincided with the finding of multiple liver masses.

[Genetic polymorphism of 16 Y-STR loci in Miao, Yao and Dong nationalities of Guangxi population].

Objective: To investigate the genetic polymorphisms of 16 Y-STR loci and to evaluate the forensic application in Miao, Yao and Dong nationalities of Guangxi population.

Methods: Genotypes of Y-STR loci were tested in a total of 253 healthy unrelated individuals (67 Miao people, 99 Yao people, 87 Dong people) using AmpFlSTR Yfiler PCR amplification kit. Allele frequencies and population genetics parameters of the 16 Y-STR loci were statistically analyzed.

[Clinical characteristics and therapy of pan-drug resistant Acinetobacter baumannii infection].

Objective: To analyze clinical characteristics and therapy of pan-resistant Acinetobacter baumannii (PDRAB) infection and explore the methods for effective therapy and prevention of this infection.

Methods: Nine hospitalized patients with PDRAB infection confirmed by pathogen and susceptibility testing were analyzed for the risk factors and the treatment outcomes were assessed by case analysis.

Results: PDRAB infections occurred mainly in patients with severe complications, most of whom had complications by diabetes or hypertension or damaged mucosal integrity due to mechanical ventilation, surgery and catheterization.

[Study of on-line CCD spectrum data acquisition system based on CPLD].

The non-invasive detection blood component has being attracted significant interest in biomedical domain, where the method of near-IR spectroscopy is special. And the dynamic spectrum method can eliminate the main interference of the individual discrepancy and measure conditions. However, due to the requirement of excellent amplitude accuracy and high temporal resolution in spectrum data acquisition system, the CCD must have real time output, the data is large, and a high speed acquisition system is required.

[Epidemiological investigation of nosocomial infection of SARS in medical staff of a hospital].

Objective: To investigate the epidemiological features of nosocomial infection of severe acute respiratory syndrome (SARS) in the medical staff of a hospital.

Methods: The medical staff associated with nosocomial infection of SARS in a hospital were investigated with standard procedures of epidemiological survey.

Results: The source of SARS infection was identified as the husband of a tumor patient who received chemotherapy in the Department of Oncology, during which they maintained intimate contact for at least two weeks when the husband had typical symptoms of SARS.