Comprehensive exploration of a traditional Chinese medicinal plant of Magnolia officinalis based on high-coverage mass spectrometry and multidimensional chemical-biological analysis.

Magnolia bark is a traditional Chinese medicine used for hypoglycaemia. With the widespread use of Magnolia bark, its resources are facing a serious shortage. To address this issue, a strategy based on high-coverage mass spectrometry (HCMS) and multidimensional chemical-biological analysis (MCBA) was proposed for the comprehensive exploration of Magnolia officinalis which is the main source of Magnolia bark.

Integration of High-Resolution LC-Q-TOF Mass Spectrometry and Multidimensional Chemical-Biological Analysis to Detect Nanomolar-Level Acetylcholinesterase Inhibitors from Different Parts of .

Zanthoxyli radix is a popular tea among the elderly, and it is believed to have a positive effect on Alzheimer's disease. In this study, a highly effective three-step strategy was proposed for comprehensive analysis of the active components and biological functions of (ZN), including high-resolution LC-Q-TOF mass spectrometry (HRMS), multivariate statistical analysis for heterogeneity (MSAH), and experimental and virtual screening for bioactivity analysis (EVBA). A total of 117 compounds were identified from the root, stem, and leaf of ZN through HRMS.

A strategy to comprehensively analyze the bioactivity of complex herbal prescriptions via peak-by-peak cutting and knock-out chromatography: Qiliqiangxin capsule as an example.

An herbal prescription is usually composed of several herbal medicines. The complex and diverse components bring great challenges to its bioactivity study. To comprehensively analyze the bioactivity of an herbal prescription, a new strategy based on peak-by-peak cutting and knock-out chromatography was proposed.

Classification-based strategies to simplify complex traditional Chinese medicine (TCM) researches through liquid chromatography-mass spectrometry in the last decade (2011-2020): Theory, technical route and difficulty.

The difficulty of traditional Chinese medicine (TCM) researches lies in the complexity of components, metabolites, and bioactivities. For a long time, there has been a lack of connections among the three parts, which is not conducive to the systematic elucidation of TCM effectiveness. To overcome this problem, a classification-based methodology for simplifying TCM researches was refined from literature in the past 10 years (2011-2020).

Genome-Wide Identification and Expression Analysis of the β-Amylase Gene Family in .

β-Amylase (BAM) is an important starch hydrolase, playing a role in a variety of plant growth and development processes. In this study, 22 gene family members (GFMs) were identified in quinoa (), an ancient crop gaining modern consumer acceptance because of its nutritional qualities. The genetic structure, phylogenetic and evolutionary relationships, and expression patterns of GFMs in different tissues, were analyzed.

[Identification of Dioscorea nipponica and its sects based on psbA-trnH barcode].

A rapid and accurate method was established by psbA-trnH sequence to distinguish Dioscorea nipponica from other species belonging to the same genus.In this study, we collected 144 samples of D. nipponica from 17 different producing areas in China, the psbA-trnH sequenced of 23 nucleotide sequences were downloaded from GenBank, the sequences from Dioscorea genus.

Ceramic-on-ceramic bearings total hip arthroplasty in young patients.

Background: The results of ceramic-on-ceramic (CoC) total hip arthroplasty (THA) in younger patients were not univocal. This study aims to evaluate the results of CoC bearing THA in patients younger than 50 years.

Methods: A total of 90 younger patients performed CoC THAs during March 2003 and May 2008 were included in this study.

Functional validation of Phragmites communis glutathione reductase (PhaGR) as an essential enzyme in salt tolerance.

Reed plants (Phragmites communis (Linn.) Trin) are hydrophilic perennial grasses growing in fresh and brackish waters. These plants readily adapt to arid and high salinity conditions; however, their resistance mechanism against abiotic stresses, especially high salinity, is largely unknown.

[Research progress on MAPK signal pathway in the pathogenesis of osteoarthritis].

Mitogen-activated protein kinases (MAPKs) signal is one of the important ways in eukaryotic cell,which adjusts and controls the structure and function of the cell. MAPKs in eukaryotes include p38, ERK, JNK and ERK5, etc. With the deepening research,we found that the activation of p38, ERK, JNK signal pathways were closely related with osteoarthritis (OA) cartilage injury.

[Research progression of interleukin-1 in the pathogenesis of osteoarthritis].

Osteoarthritis is a common joint disease, which seriously affects the patient's health and quality of life. It results in substantial social and economic costs. Etiology and pathogenesis of OA is still not completely clear, but people paid more attention on Cytokines, especially IL-1, which is considered as core factor in the development of OA.

[Optimal extraction of polysaccharide from Dioscorea nipponica by central composite design and response surface methodology].

Objective: To optimize the extracting technology for polysaccharides from Dioscorea nipponica by response surface methodology.

Methods: The independent variables were extraction temperature, reflux time and liquid-to-solid ratio, the dependent variable was extraction rate of polysaccharides in Dioscorea nipponica. Linear or nonlinear mathematic models were used to estimate the relationship between independent and dependent variables.

[The evolution of related names of Bi syndrome and the theory of etiology and pathogenesis].

In the Traditional Chinese medical literature of ancient times, Bi referred to the pathogenesis, or the symptoms as well as the name of the disease. As the name of a disease, Bi has the different meanings of broad and narrow., joint-running, joint-running wind, white tiger joint-running, gout etc.

Overexpression of SOS (Salt Overly Sensitive) genes increases salt tolerance in transgenic Arabidopsis.

Soil salinity is a major abiotic stress that decreases plant growth and productivity. Recently, it was reported that plants overexpressing AtNHX1 or SOS1 have significantly increased salt tolerance. To test whether overexpression of multiple genes can improve plant salt tolerance even more, we produced six different transgenic Arabidopsis plants that overexpress AtNHX1, SOS3, AtNHX1+SOS3, SOS1, SOS2+SOS3, or SOS1+SOS2+SOS3.

[Identification on Geranium by X-ray diffraction fourier fingerprint patterns and similarity degree of different fingerprint patterns].

Objective: To provide a X-ray diffraction (XRD) new method to identify different of herbal drugs of Geranium.

Methods: Analyse X-ray diffraction Fourier pattern of 1 plants of Geranium, 7 kinds substitute, and degree of different fingerprint patterns was calculated and analyzed according to the position (2 theta value) of peaks searched.

Results: Obtain the different X-ray diffraction Fourier pattern of 1 kinds plants of Geranium and 7 kinds substitutes of Geranium.

[Effect of compound duzhong jiangu granule on knee joint osteoarthritis: a report of 400 cases].

Objective: To study the clinical efficacy and adverse reaction of compound Duzhong Jiangu granule (CDJG) on knee joint osteoarthritis (KJO) of Gan-Shen deficiency with stasis in tendon and muscle syndrome.

Methods: The randomized controlled method was adopted in this study, comparative study was conducted in 400 patients in the treated group treated with CDJG and 200 patients in the control group treated with Zhuanggu Guanjie pill (ZGP).

Results: The total effective rate in the treated group was 92% and the curative-markedly effective rate was 47%, which were higher than those in the control group respectively.