Substrate Characterization for Hydrolysis of Multiple Types of Aromatic Esters by Promiscuous Aminopeptidases.

Synthetic aromatic esters, widely employed in agriculture, food, and chemical industries, have become emerging environmental pollutants due to their strong hydrophobicity and poor bioavailability. This study attempted to address this issue by extracellularly expressing the promiscuous aminopeptidase (Aps) from GF31 in , achieving an impressive enzyme activity of 13.7 U/mg.

Luminol-Eu-Gd-functionalized mesoporous silica for ultrasensitive detection of tetracycline antibiotics and smartphone-assisted sensing analysis.

An organic-inorganic hybrid nanoprobe, namely LML-D-SBA@Eu-Gd, was constructed, with SBA-15 acting as the carrier material, and luminol and Eu acting as fluorescence channels to achieve ratiometric signals that eliminate external interference (accurate detection). Gd was used as a sensitizer to amplify the red emission of Eu (ultrasensitive detection). In TCs detection, the luminol emission at 428 nm was quenched due to the photoinduced electron transfer mechanism, and the Eu emission at 617 nm was sensitized due to the synergistic energy transfer from TCs and Gd to Eu.

A novel lncRNA TCONS_00071187 upregulated by activated GSK3β promotes high glucose-induced mesangial cell proliferation in the diabetic nephropathy.

Inhibiting mesangial cell proliferation is one of the strategies to control the early progression of diabetic nephropathy (DN). GSK3β is closely related to cell apoptosis as well as the development of DN, but whether it acts on the proliferation of mesangial cells is unclear. This study aimed to elucidate the role and mechanism of GSK3β-mediated lncRNA in high glucose-induced mesangial cell proliferation.

Visible light-promoted anti-biofouling performance of cellulose acetate membrane for reverse osmosis desalination.

Sea water desalination is regarded as a major solution that could alleviate the water scarcity problem. Reverse osmosis (RO) is typically employed to recover fresh water from sea and brackish water via economical means. RO membrane fouling remains a critical issue restricting their widespread application.

Antifungal activity of the repurposed drug disulfiram against .

Fungal infections have become clinically challenging owing to the emergence of drug resistance in invasive fungi and the rapid increase in the number of novel pathogens. The development of drug resistance further restricts the use of antifungal agents. Therefore, there is an urgent need to identify alternative treatments for ().

Anti-adhesion multifunctional poly(lactic-co-glycolic acid)/polydimethylsiloxane wound dressing for bacterial infection monitoring and photodynamic antimicrobial therapy.

Wound infection and adhesion are important factors affecting wound healing. Early detection of pathogen infection and reduction of wound-to-dressing adhesion are critical for improving wound healing. Herein, Ester-J, which can rapidly respond to lipase secreted by bacteria, was designed and synthesized.

A patient with acute myocardial infarction with electrocardiogram Aslanger's pattern.

Background: Aslanger's pattern in electrocardiogram (ECG) indicates that patients may have acute inferior myocardial infarction(AMI) with concomitant critical stenoses on other coronary arteries, which needs to be evaluated the timing of revascularization as risk equivalents of ST elevation myocardial infarction(STEMI).

Case Presentation: The patient was a 62-year-old male with chief complaint of intermittent exertional subxiphoid pain for 20 days from 30th June. One day after the last episode (19th July), the 18-lead electrocardiogram showed ST segment elevation of 0.

Pyrene and Eu modified mesoporous silica used as a fluorescent probe for ratiometric detection of tetracycline antibiotics.

The detection of residual tetracycline antibiotics (TCs) in the aquatic environment is important because of the irreversible damage of TCs to the ecosystem and human health. Considering the photostability, sensitivity, and accuracy of the detection systems, mesoporous SBA-15 silica material was an ideal support matrix for improving photostability because it can protect the optical properties of fluorescent signals. To achieve ratiometric detection (with sensitivity and accuracy), pyrene and Eu as two fluorescent signals were implemented into SBA-15, and an organic-inorganic hybrid fluorescent nanoprobe Py/EDTA-SBA-15@Eu was constructed.

A smartphone-based fluorescent sensor for rapid detection of multiple pathogenic bacteria.

In this study, we developed a fluorescent sensor for the sensitive detection of multiple pathogenic bacteria based on magnetic separation, fluorescent probes, and smartphone image processing. A microchannel device was assembled using high-transparency resin and 3D printing technology. This device was combined with a smartphone and an external lens to develop a fluorescent sensor for autonomous detection of multiple pathogenic bacteria.

Pterostilbene alleviated cerebral ischemia/reperfusion-induced blood-brain barrier dysfunction inhibiting early endothelial cytoskeleton reorganization and late basement membrane degradation.

Pterostilbene, an important analogue of the star molecule resveratrol and a novel compound naturally occurring in blueberries and grapes, exerts a significant neuroprotective effect on cerebral ischemia/reperfusion (I/R), but its mechanism is still unclear. This study aimed to follow the molecular mechanisms behind the potential protective effect of pterostilbene against I/R induced injury. For fulfilment of our aim, we investigated the protective effects of pterostilbene on I/R injury caused by middle cerebral artery occlusion (MCAO) and oxygen-glucose deprivation (OGD) .

Dispelling Dampness, Relieving Turbidity and Dredging Collaterals Decoction, Attenuates Potassium Oxonate-Induced Hyperuricemia in Rat Models.

Purpose: Dispelling dampness, relieving turbidity and dredging collaterals decoction (DED), is a traditional Chinese medicine used in the treatment of hyperuricemia. We aimed to explore the effect and mechanism of DED in the treatment of hyperuricemia.

Methods: The effects of DED (9.

Rhodamine derivative-functionalized mesoporous silica-Al hybrid material for fluorescence "turn-on" detection of tetracycline antibiotics in aqueous media.

The organic-inorganic hybrid material was prepared by embedding 2-amino-3',6'-bis(diethylamino)spiro[isoindoline-1,9'-xanthen]-3-one (RBH) onto mesoporous SBA-15 silica and coordinating it with Al (RBH-SBA-15-Al). RBH-SBA-15-Al was used for the selective and sensitive detection of tetracycline antibiotics (TAs) in aqueous media based on the binding site-signaling unit mechanism, in which Al acted as the binding site and the fluorescence intensity at 586 nm as the response signal. The addition of TAs to RBH-SBA-15-Al suspensions resulted in the formation of RBH-SBA-15-Al-TAs conjugates, which realized the electron transfer process and turned-on fluorescence signal at 586 nm.

An effective and chemotherapy-free strategy of all-trans retinoic acid and arsenic trioxide for acute promyelocytic leukemia in all risk groups (APL15 trial).

The combination of all-trans retinoic acid (ATRA) and arsenic trioxide (ATO) has been demonstrated to have comparable effectiveness or better to ATRA and chemotherapy (CHT) in non-high-risk acute promyelocytic leukemia (APL). However, the efficacy of ATRA-ATO compared to ATRA-ATO plus CHT in high-risk APL remains unknown. Here we performed a randomized multi-center non-inferiority phase III study to compare the efficacy of ATRA-ATO and ATRA-ATO plus CHT in newly diagnosed all-risk APL to address this question.

Fluorescence ''turn-on'' probe for the selective detection of water in organic solvents based on functionalized mesoporous silica.

A fluorescence ''turn-on'' probe, namely, TTA-SBA-15, for the selective detection of water (HO) was designed by grafting terthiophene fluorophore onto ethylenediamine functionalized mesoporous SBA-15 silica. The maximum fluorescence emission peak of TTA-SBA-15 ranged from 462 nm (toluene) to 525 nm (methanol) in various organic solvents. No fluorescence was observed in HO due to the donor-excited photoinduced electron transfer mechanism, in which terthiophene acted as the donor and the amino group acted as the acceptor.

Subchronic Oral Toxicity Evaluation of Sodium Dehydroacetate: A 90-day Repeated Dose Study in Rats.

Objective: The present study was undertaken to evaluate the subchronic oral toxicity of sodium dehydroacetate (DHA-Na) and to determine the point of departure (POD), which is a critical factor in the establishment of an acceptable dietary intake.

Methods: DHA-Na was administered once daily by gavage to Sprague-Dawley rats at dose levels of 0.0, 31.

Introducing electrolysis to enhance anaerobic digestion resistance to acidification.

The phenomenon that the anaerobic system is inhibited by acid has always been a bottleneck hindering the application of anaerobic digestion (AD) technology. We tried to introduce electrolysis into AD to improve the acidification resistance, and eventually the productivity of the energy. In a batch fermentation device, the ability of electrochemical anaerobic digestion (EAD) to resist acidification was evaluated in current intensity, electrode potential, AC impedance, microbial community, pH value, and volatile fatty acids (VFAs).

Terthiophene-functionalized mesoporous silica-based fluorescence sensor for the detection of trace methyl orange in aqueous media.

A terthiophene-functionalized mesoporous SBA-15 silica, i.e., TTU-SBA-15, was successfully developed and used as a highly selective and ultrasensitive fluorescence sensor for methyl orange (MO) detection.

Multifunctional wound dressing for rapid hemostasis, bacterial infection monitoring and photodynamic antibacterial therapy.

Wound management is a major global issue, and there is a growing challenge to develop more effective hemostatic dressings to control bleeding and prevent pathogen infections. In this study, a multifunctional wound dressing was developed to meet the clinical need. The hemostatic layer of wound dressing can quickly stop the bleeding.

Comparison of anti-inflammatory effects of and based on network pharmacology.

Objective: In Chinese herbal medicine (CHM) history, and were used clinically as one drug, but now they are admitted as two herbal medicines in (2010 edition). This study used network pharmacology to investigate whether the two can be used interchangeably for the treatment of inflammatory diseases in TCM clinical practice.

Methods: and were compared in the inflammation mechanism including core targets, Gene Ontology (GO), pathway and principle chemical components by the method of network pharmacology.

A novel double target fluorescence probe for Al/Mg detection with distinctively different responses and its applications in cell imaging.

The metal cations, Al and Mg, could affect human health and cell biological processes. Their fast and selective detection using one probe remains a challenge. A novel fluorescence probe, N'-((1-hydroxynaphthalen-2-yl)methylene)isoquinoline-3-carbohydrazide (NHMI), was developed for selectively monitoring Al and Mg.

Coronary artery disease: differential expression of ceRNAs and interaction analyses.

Background: Previous studies have demonstrated associations between cardiovascular disease and the expression of various messenger RNAs (mRNAs), microRNAs (miRNAs), and long non-coding RNAs (lncRNAs). This study aimed to investigate the differential expression of mRNAs, lncRNAs, and miRNAs between tissues from patients with coronary artery disease (CAD) and healthy controls, and to determine the interactions between these molecules in CAD.

Methods: We investigated the differential expression of competitive endogenous RNAs (ceRNAs) between patients with CAD and healthy controls by collecting data from Gene Expression Omnibus (GEO) microarrays.

N-hydroxypropyl substituted 4-hydroxynaphthalimide: Differentiation of solvents and discriminative determination of water in organic solvents.

A naphthalimide-based fluorophore (HONIOH) was designed by introducing a hydroxy unit into the 4th position of the aromatic core and a hydroxypropyl unit into the N-imide site. Photophysical properties of HONIOH were highly dependent on solvents, which was ascribed to the excited state proton transfer (ESPT) coupled with intramolecular charge transfer (ICT) mechanism. Further studies demonstrated that HONIOH can be used to recognize N, N-dimethylformamide (DMF) qualitatively and differentiate methanol from ethanol.