[Effects of different color paper bags on volatile constituents of Kyoho grape berries].

Taking'Kyoho' as the test material, the effect of white, green, red and blue bags on volatile compounds in mature grape berries was explored in this study. The results indicated that bags with different colors could create special light conditions, which resulted in the difference of volatile compounds in grape berries. A total of 37, 38, 32, 34 kinds of volatile compounds which included esters, aldehydes, alcohols, ketones, terpenes and aromatic compounds, were detected in 'Kyoho' berries covered with the white, green, red and blue bags respectively, and 33 in the control.

Can K-Line Predict the Clinical Outcome of Anterior Controllable Antedisplacement and Fusion Surgery for Cervical Myelopathy Caused by Multisegmental Ossification of the Posterior Longitudinal Ligament?

Objective: To analyze the correlation between the K-line-based classification of patients with ossification of the posterior longitudinal ligament (OPLL) and their outcome after anterior controllable antedisplacement and fusion (ACAF) surgery.

Methods: A series of 24 patients with multisegmental OPLL were enrolled. All patients underwent ACAF surgery.

[Effects of different rootstocks on the weak light tolerance ability of summer black grape based on 4 photo-response models].

The photo response curves of 11 rootstock-scion combinations including summer black/Beta, summer black/1103P, summer black/101-14, summer black/3309C, summer black/140Ru, summer black/5C, summer black/5BB, summer black/420A, summer black/SO4, summer black/Kangzhen No.1, summer black/Huapu No.1 were fitted by rectangular hyperbola mo-del, non-rectangular hyperbola model, modified rectangular hyperbola model and exponential model respectively, and the differences of imitative effects were analyzed by determination coefficiency, light compensation point, light saturation point, initial quantum efficiency, maximum photosynthetic rate and dark respiration rate.

[Effects of light quality on leaf senescence and endogenous hormones content in grapevine under protected cultivation].

Two grape varieties 'Italia' and 'Centenial seedless' cultured in protected and delayed cultivation were used as experimental materials to study the effects of red and blue light quality on chlorophyll content, net photosynthetic rate, and endogenous hormone content during leaf senescence. The results showed that the chlorophyll content and net photosynthetic rate of the grapes were significantly enhanced in the red light treatment. Although the content of endogenous GA was decreased, an increase in abscisic acid (ABA) content and a decrease of total content of zeaxanthin (ZR) were obviously slowed, which led to the significant increase in value of (GA+ZR)/ABA, delaying leaf senescence.

Glycated Apolipoprotein A-IV Induces Atherogenesis in Patients With CAD in Type 2 Diabetes.

Background: Nonenzymatic glycation of apolipoproteins plays a role in the pathogenesis of the vascular complications of diabetes.

Objectives: This study investigated whether apolipoprotein (apo) A-IV was glycated in patients with type 2 diabetes mellitus (T2DM) and whether apoA-IV glycation was related to coronary artery disease (CAD). The study also determined the biological effects of glycated apoA-IV.

[Effects of supplementary light with different wavelengths on fruit quality of 'Ruidu Xiangyu' grape under promoted cultivation].

To study the effects of supplemental lighting with ultraviolet (UV), blue and red-blue lights on quality development of grape berries under protected cultivation to promote early maturing, four-year-old vines of 'Ruidu Xiangyu' grape using 'Beta' as rootstock were used in this experiment. The results indicated that blue and ultraviolet lights were poor in greenhouse environment. Compared with the control, blue and ultraviolet lights lasting 6 hours at night significantly accele-rated the increase of berry mass, fruit diameter, sugar content and the decrease of acidity content during the grape development, but the effect of red-blue light was not obvious.

Increment of HFABP Level in Coronary Artery In-Stent Restenosis Segments in Diabetic and Nondiabetic Minipigs: HFABP Overexpression Promotes Multiple Pathway-Related Inflammation, Growth and Migration in Human Vascular Smooth Muscle Cells.

Background: Our previous study suggested that heart-type fatty acid-binding protein (HFABP) levels were greatly elevated in the conditioned medium of explant culture of in-stent restenosis (ISR) tissue from diabetic minipigs compared with those of non-ISR tissue. We here verified this result in animal tissues and investigated the impact of HFABP overexpression in human aortic smooth muscle cells (hASMCs).

Methods And Results: In Western blot and real-time RT-PCR, HFABP protein and mRNA levels were significantly higher in ISR than in non-ISR tissues from minipigs, and higher in the ISR tissue from diabetic minipigs than that from nondiabetic minipigs.

C1q/TNF-related protein-1: an adipokine marking and promoting atherosclerosis.

Aims: We investigated the association of the adipokine C1q/TNF-related protein (CTRP) 1 with coronary artery disease (CAD), and the biological vascular effects of CTRP1.

Methods And Results: We analysed CTRP1 levels in sera of CAD patients (n = 451) and non-CAD controls (n = 686), and in coronary endarterectomy specimens (n = 32), non-atherosclerotic internal mammary arteries (n = 26), aortic atherosclerotic plaques (n = 15), and non-atherosclerotic aortic samples (n = 10). C1q/TNF-related protein-levels were higher in sera, endarterectomy specimens, aortic atherosclerotic plaques, and peripheral blood mononuclear cells (PBMCs) from CAD patients compared with controls, and were related to CAD severity.

Evaluation of microbially enhanced composting of sophora flavescens residues.

The effects of inoculants on the composting of Sophora flavescens residues were evaluated based on several physical, chemical and biological parameters, as well as the infrared spectra. Compared to the control compost without inoculants, the treatment compost with inoculants (Bacillus subtilis strain G-13 and Chaetomium thermophilum strain GF-1) had a significantly longer thermophilic duration, higher cellulase activity and a higher degradation rate of cellulose, hemicellulose and lignin (P < 0.05).

p-Cresyl sulfate is associated with carotid arteriosclerosis in hemodialysis patients and promotes atherogenesis in apoE-/- mice.

p-Cresyl sulfate (PCS) is a risk factor of cardiovascular disease in patients with chronic kidney disease. Here we tested whether serum PCS levels were related to the rate and evolution of carotid atherosclerosis in hemodialysis patients and identified a potential mechanism. A total of 200 hemodialysis patients were categorized as with or without carotid atherosclerotic plaque and followed for 5 years.

HIV-infected female sex workers' high risk behavior and attitude changes in Kaiyuan City, Yunnan Province, China.

Objective: To investigate the attitude and sexual behavior status and change among HIV positive female workers in entertainment sites in Kaiyuan city, Yunnan province, China. The key information should be applied in the integrated intervention program in future.

Methods: A cohort survey among HIV positive female workers was conducted during 12 months, between 2010 and 2012.

Antimicrobial susceptibility of Streptococcus sp. to quinupristin-dalfopristin in China.

This study aimed to determine the in vitro activity of quinupristin-alfopristin against Streptococcus sp. isolated in China. This agent is not yet available for clinical use, but it has been tested against a high proportion of resistant Staphylococcus aureus strains.

Rapid and high-throughput identification of recombinant bacteria with mass spectrometry assay.

Objective: To construct a rapid and high-throughput assay for identifying recombinant bacteria based on mass spectrometry.

Methods: Matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) techniques were used to identify 12 recombinant proteins (10 of Yersinia pestis, 1 of Campylobacter jejuni and 1 of Helicobacter pylori). A classification model for the various phase of recombinant bacteria was established, optimized and validated, using MALDI-TOF MS-ClinProTools system.

Molecular epidemiological characteristics of Streptococcus pyogenes strains involved in an outbreak of scarlet fever in China, 2011.

Objective: To investigate molecular characterization of streptococcus pyogenes isolates involved in an outbreak of scarlet fever in China in 2011.

Methods: Seventy-four Streptococcal pyogenes involved in an outbreak of scarlet fever were isolated from pediatric patients in the areas with high incidence in China from May to August of 2011. Emm genotyping, pulsed-field gel electrophoresis (PFGE), superantigen (SAg) genes and antimicrobial susceptibility profiling were analyzed for these isolates.

Pyruvate Ringer's solution corrects lactic acidosis and prolongs survival during hemorrhagic shock in rats.

Background: Lactic acidosis is a life-threatening complication of hemorrhagic shock. There is no ideal therapy for it in the clinical setting.

Objective: This study was designed to investigate whether pyruvate Ringer's solution could treat hypoxic lactic acidosis associated with lethal hemorrhagic shock in rats.

Preclinical studies of targeted therapies for CD20-positive B lymphoid malignancies by Ofatumumab conjugated with auristatin.

Utilization of antibodies to deliver highly potent cytotoxic agents to corresponding antigen-overexpressed tumor cells is a clinically validated therapeutic strategy. Ofatumumab (OFA, trade name Arzerra) is a fully human CD20-specific antibody that is active against CD20-positive B-cell lymphoma/chronic lymphocytic leukemia cells. In order to further enhance the anticancer effect of OFA, anti-CD20 OFA has been conjugated with highly cytotoxic monomethyl auristatin E (MMAE) through a cathepsin-B-cleavable valine-citrulline (vc) dipeptide linkage to form OFA-vcMMAE and the anti-tumor activity of OFA-vcMMAE against CD20-positive B lymphoma cells are then evaluated in vitro and in vivo.

Herpes simplex virus type 2 risks in female sex workers in the China-Vietnam border county of Hekou.

Objective: To survey the prevalence and risk factors of HSV-2 among Chinese and Vietnamese female sex workers (FSW) in the border county of Hekou, Yunnan Province, China.

Methods: A cross-sectional survey was conducted on demographics, sexual behavior, medical history, and drug use among FSWs. Laboratory samples were obtained to test for HSV-2 and other STIs such as HIV, Syphilis, Trichomonas vaginalis, Neisseria gonorrhoeae, Chlamydia trachomatis, Bacterial vaginosis, and Yeast infections.

Reduced serum levels of vasostatin-2, an anti-inflammatory peptide derived from chromogranin A, are associated with the presence and severity of coronary artery disease.

Aims: We here investigated the endothelial effects of the chromogranin A-derived peptide vasostatin-2 and its relation to coronary artery disease (CAD).

Methods And Results: We assessed the impact of recombinant vasostatin-1 and vasostatin-2 on tumour necrosis factor-alpha (TNFα)-, angiotensin II-, and oxidized low-density lipoprotein (oxLDL)-induced expression of adhesion molecules in human arterial endothelial cells. Vasostatin-1 and vasostatin-2 levels were examined in coronary endarterectomy specimens (n= 23), atherosclerotic aortas (n= 16), non-significant-atherosclerotic internal mammary arteries (n= 30), and non-atherosclerotic aortas (n= 10), as well as in peripheral blood mononuclear cells (PBMCs) from severe CAD patients (n= 50) and healthy volunteers (n= 21).

Separation of allelopathy from resource competition using rice/barnyardgrass mixed-cultures.

Plant-plant interference is the combined effect of allelopathy, resource competition, and many other factors. Separating allelopathy from resource competition is almost impossible in natural systems but it is important to evaluate the relative contribution of each of the two mechanisms on plant interference. Research on allelopathy in natural and cultivated plant communities has been hindered in the absence of a reliable method that can separate allelopathic effect from resource competition.

[Dicer interacts with Wig-1 protein].

Dicer is a key dsRNase in RNA interference, and Wig-1 is a p53-induced zinc finger protein with dsRNA-binding activity. In this report, we demonstrated a direct interaction between Dicer and Wig-1. Dicer associated with Wig-1, which was expressed as a glutathione S-transferase fusion protein, in a dose-dependent manner by GST pull-down assay.

[Reduction of tumorigenicity of SMMC7721 hepatoma cell line by VEGF siRNA].

In this study the small interfering RNA(siRNA) targeting human VEGF effectively inhibited the expression of VEGF in human hepatoma cell line, SMMC7721, and could dramatically decrease the tumorigenicity of SMMC7721 s.c. xenograft tumor.

Construction and characterization of a goat mammary gland cDNA library.

A lactating goat mammary gland cDNA library was constructed by using a modified commercially available cDNA library construction kit protocol. The resulting clones were sequenced and functionally analyzed through cross-species genomic comparison to assess (1) the capacity and functional quality of the constructed library for subsequent research and (2) the efficiency of the procedural modifications. The study resulted in the construction of a high-quality mammary gland cDNA library, which was characterized by (1) the total recombinants number of 1.