Tunisian Multicenter Study on the Prevalence of Colistin Resistance in Clinical Isolates of Gram Negative Bacilli: Emergence of Harbouring the Gene.

Background: Actually, no data on the prevalence of plasmid colistin resistance in Tunisia are available among clinical bacteria.

Objectives: This study aimed to investigate the current epidemiology of colistin resistance and the spread of the gene in clinical Gram-negative bacteria (GNB) isolated from six Tunisian university hospitals.

Methods: A total of 836 GNB strains were inoculated on COL-R agar plates with selective screening agar for the isolation of GNB resistant to colistin.