Quantitative evaluation of software packages for single-molecule localization microscopy.

The quality of super-resolution images obtained by single-molecule localization microscopy (SMLM) depends largely on the software used to detect and accurately localize point sources. In this work, we focus on the computational aspects of super-resolution microscopy and present a comprehensive evaluation of localization software packages. Our philosophy is to evaluate each package as a whole, thus maintaining the integrity of the software.

FALCON: fast and unbiased reconstruction of high-density super-resolution microscopy data.

Super resolution microscopy such as STORM and (F)PALM is now a well known method for biological studies at the nanometer scale. However, conventional imaging schemes based on sparse activation of photo-switchable fluorescent probes have inherently slow temporal resolution which is a serious limitation when investigating live-cell dynamics. Here, we present an algorithm for high-density super-resolution microscopy which combines a sparsity-promoting formulation with a Taylor series approximation of the PSF.

Adaptive image resizing based on continuous-domain stochastic modeling.

We introduce an adaptive continuous-domain modeling approach to texture and natural images. The continuous-domain image is assumed to be a smooth function, and we embed it in a parameterized Sobolev space. We point out a link between Sobolev spaces and stochastic auto-regressive models, and exploit it for optimally choosing Sobolev parameters from available pixel values.

On the role of exponential splines in image interpolation.

A Sobolev reproducing-kernel Hilbert space approach to image interpolation is introduced. The underlying kernels are exponential functions and are related to stochastic autoregressive image modeling. The corresponding image interpolants can be implemented effectively using compactly-supported exponential B-splines.