Publications by authors named "Hafiz M Rizwan"

Background: Zinc finger homeodomain (ZF-HD) belongs to the plant-specific transcription factor (TF) family and is widely involved in plant growth, development and stress responses. Despite their importance, a comprehensive identification and analysis of ZF-HD genes in the soybean (Glycine max) genome and their possible roles under abiotic stress remain unexplored.

Results: In this study, 51 ZF-HD genes were identified in the soybean genome that were unevenly distributed on 17 chromosomes.

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A total of 384 animals (sheep, goat, cattle, and buffalo) were examined for the presence of hydatid cysts only in the lungs. The lung tissue samples associated with the hydatid cyst were collected immediately after slaughter, followed by fixation in 10% formalin. The fixed tissue was subjected to paraffin embedding technique.

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Entomopathogenic fungi (EPFs) can infect and kill a diverse range of arthropods, including ticks (Acari: Ixodidae) that can transmit various diseases to animals and humans. Consequently, the use of EPFs as a biocontrol method for managing tick populations has been explored as an alternative to chemical acaricides, which may have harmful effects on the environment and non-target species. This review summarizes studies conducted on EPFs for tick control between 1998 and 2024, identifying 9 different EPF species that have been used against 15 different species of ticks.

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Light-harvesting chlorophyll a/b-binding proteins (LHC) of photosystem II perform key functions in various processes, e.g., photosynthesis, development, and abiotic stress responses.

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  • The study aimed to evaluate how effective and safe second-line anti-TB drugs are for patients with multidrug-resistant tuberculosis (MDR-TB) in a hospital in South Punjab over a 24-month period.
  • It included a review of patient records to track important health metrics like weight and sputum smear results, while noting side effects experienced during treatment.
  • Results showed significant improvement in both treatment effectiveness (with more negative sputum smears) and patient weight gain, but also revealed some mild to moderate adverse drug reactions that require careful monitoring.
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For a sustainable ecology, slaughterhouse sludge must be managed effectively in preview of the parasitic or coliforms' spill over to the community. In order to determine the effectiveness of a customized biological decomposer solution in lowering the parasitic eggs and coliform bacteria, three composting units (Unit 1, Unit 2, and Unit 3) were treated with its different amounts. Over a period of 60 days, pH, temperature, humidity, number of the parasitic eggs per gram (EPG) of faecal material, viability of eggs, and coliform counts were evaluated.

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  • A study in Narowal, Punjab, found that 72.92% of ruminants slaughtered were infected with gastrointestinal parasites, with goats having the highest infection rate at 78.63%.
  • Female ruminants experienced significantly higher infection rates (85.62%) than males (65.13%).
  • The research revealed a severe economic impact, estimating annual losses of approximately Pak. Rs. 405.09 million (around USD 1.43 million) due to organ condemnation from GI parasites.
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  • - The study investigates the relationship between meteorological factors (temperature, humidity, and rainfall) and the abundance of mosquitoes, which are essential vectors for malaria-causing Plasmodium parasites.
  • - Culex mosquitoes were found to be more abundant (57.65%) compared to Anopheles (42.34%), with a peak population observed in March under specific climatic conditions in Mandi Bahauddin.
  • - Molecular testing revealed that 41.3% of female Anopheles mosquitoes carried Plasmodium, with P. vivax (78.1%) being more prevalent than P. falciparum (21.9%), providing valuable insights for future mosquito-borne disease risk assessment and control strategies. *
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Protein glycation in human body is closely linked to the onset/progression of diabetes associated complications. These glycated proteins are commonly known as advanced glycation end products (AGEs). Recent literature has also highlighted the involvement of AGEs in other non-communicable diseases (NCDs) such as cardiovascular, cancer, and Alzheimer's diseases and explored the impact of plant metabolites on AGEs formation.

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Enhancing enzyme activity and stability in biomass degradation can improve substrate saccharification and, increases biorefinery efficiency. For the first time, we identified 20 lytic polysaccharide monooxygenases (LPMOs) AA9 genes in the genome of Thermothelomyces fergusii. Our results showed that TfAA9 was categorized into LPMOs1, LPMOs2, and LPMOs3 subgroups based on protein diversity.

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  • The study investigates the prevalence of theileriosis, a tick-borne disease affecting buffalo and cattle in Toba Tek Singh, Pakistan, with significant economic implications globally.
  • DNA screening revealed various tick species, with an overall infection rate of 35.9% in large ruminants' blood samples, and higher rates of infection found in female ticks and those from riverine environments.
  • Findings indicate that cattle are more affected than buffalo (43.2% vs. 28.7%), and factors like age, sex, and habitat significantly influence infection rates, providing critical data for risk assessment in livestock.
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We started a campaign in the heart of Faisalabad, Punjab, Pakistan, to expose the hidden threats of parasitic illnesses in ruminants and the severe financial consequences associated with them. Our in-depth investigations focused on the prevalence, impact, and astounding financial losses brought on by organ contamination in slaughtered animals. Of the 384 slaughtered ruminants examined for gastrointestinal parasites, a prevalence of 44.

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  • This study explored the relationship between trace minerals in sheep forages and their gastrointestinal (GI) parasite burdens, aiming to find a connection between the two.
  • Researchers collected 384 fecal samples and analyzed trace minerals such as Mn, Co, Cu, and Zn in both the forages and sheep serum, discovering significant variations between the two districts, Sialkot and Multan.
  • Findings showed a prevalence of GI parasites at 34% in Sialkot and 32% in Multan, with certain trace minerals correlating with parasite egg counts, suggesting that improving forage mineral content could help manage GI parasites in sheep.
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Passion fruit is an essential commercial plant in the tropics and subtropics, which has lately seen a rise in demand for high-quality fruits and large-scale production. Generally, different species of passion fruit (Passiflora sp.) are propagated by sexual reproduction.

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Background: Among the blood-borne parasitic infections of dogs, trypanosomosis and babesiosis are highly prevalent in the Indian subcontinent and highly pathogenic.

Methods: A six-month-old dog was presented with complaint of lethargy, anorexia and loss of body weight over the previous 15 days on October 11, 2021. A full clinical and targeted haematologic and parasitological examination was undertaken.

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Avian coccidiosis is caused by genus i.e. and and lead to three billion US dollar per year economic loss in poultry industry and reduces the growth performance of birds.

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Introduction: (an intracellular protozoan) causes toxoplasmosis in warm-blooded animals, including humans and dogs. The present study was carried out to investigate the seroprevalence of canine toxoplasmosis in the owned and stray populations of dogs in Faisalabad District, Punjab, Pakistan.

Materials And Methods: Commercially available Latex Agglutination Test (LAT) kits were used for the screening of samples (139 stray and 150 owned), followed by confirmation through ELISA.

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The gene family is one of the largest plant transcription factors (TFs) families and plays important roles in plant growth, development, metabolism, and biotic and abiotic stresses. However, gene family has not been reported in passion fruit (). In this study, a total of 105 genes were identified in the passion fruit genome and were unevenly distributed across all nine-passion fruit chromomere, with a maximum of 48 genes on chromosome one.

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Objectives: To evaluate and compare the effects of redo percutaneous mitral valvuloplasty with initial percutaneous mitral valvuloplasty (PMV) in mitral restenosis (MR) and de novo mitral stenosis (MS) patients, respectively.

Methods: A retrospective study was conducted at the cardiology department of Ch. Pervaiz Elahi Institute of Cardiology Multan for the period of one year from 6 July 2020 to 6 July 2021.

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  • * Phylogenetic analysis categorized the genes into seven clades, revealing evolutionary relationships, while gene structure analysis showed a variety of exons and conserved motifs.
  • * Key findings indicated potential roles of genes in plant growth and stress response, with gene duplication and interactions with specific miRNAs and transcription factors also contributing to the biological processes.
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Juice sac granulation (a physiological disorder) leads to large postharvest losses of pomelo (). Previous studies have shown that juice sac granulation is closely related to lignin accumulation, while the molecular mechanisms underlying this disorder remain elusive in pomelo. Our results showed that the lignin content in NC (near the core) and FC (far away from the core) juice sacs overall increased from 157 DPA (days post anthesis) to 212 DPA and reached a maximum at 212 DPA.

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  • * There are about 38 viral species linked to ticks, with most being RNA viruses, and the interaction between ticks and their hosts is critical for effective pathogen transmission, facilitated by the ticks' saliva which contains proteins essential for virus uptake and reproduction.
  • * Immunological factors, such as specific receptors and pathways, significantly influence tick-virus interactions, and ticks can also harbor other viruses in their internal microbial communities, making the study of these interactions vital for understanding the spread of tick
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Plant and fruit surfaces are covered with cuticle wax and provide a protective barrier against biotic and abiotic stresses. Cuticle wax consists of very-long-chain fatty acids (VLCFAs) and their derivatives. β-Ketoacyl-CoA synthase () is a key enzyme in the synthesis of VLCFAs and provides a precursor for the synthesis of cuticle wax, but the gene family was yet to be reported in the passion fruit ().

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Passion fruit () is an important fruit crop with high economic value. Genetic engineering plays an important role in crop improvement with desired traits and gene functional studies. The lack of a simple, efficient, and stable transformation system for passion fruit has greatly limited gene functional studies.

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Flavonoids play a key role as a secondary antioxidant defense system against different biotic and abiotic stresses, and also act as coloring compounds in various fruiting plants. In this study, fruit samples of purple ( f. ) and yellow ( f.

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