Lipolysis in homogenates of adipose tissue: an inhibitor found in fat from obese rats.

The presence of a lipidbound inhibitor in adipose tissue of rats with hypothalamic obesity may explain the failure of the tissue to release fatty acids on epinephrine stinmulation. Aqueous extracts of tissue from obese animals showed no deficiency of lipase activity, but when whole homogenates of epididymal fat from lean and obese animals were mixed, 25 percent tissue from obese animals reduced by 73 percent the release expected from tissue of lean controls.

THE RENAL ELIMINATION OF BILIRUBIN.

The elimination of bile pigment during jaundice is, for practical purposes, unincreased by diuresis from water by mouth. Possibly, though, the flushing of the kidneys tends to lessen pigment accumulation within these organs and thus to diminish a serious potential source of trouble in long continued jaundice. Flood diuresis from intravenous injections of salt solution markedly increases the output of bile pigment.

THE EFFECT OF FLOOD DIURESIS ON HEMOGLOBINURIA.

Flood diuresis so far lowers the renal threshold for hemoglobin that the pigment appears in quantity in the urine as result of a hemoglobinemia insufficient under ordinary circumstances to lead to the elimination of even a trace of it. In pathological conditions that involve blood destruction hemoglobin probably passes into the tubules much more often than it reaches the urine, being prevented therefrom by the resorptive activity of the tubular epithelium.

THE FACTOR DETERMINING THE SPREAD OF RED MARROW DURING ANEMIA.

Rabbits in which a chronic anemia of moderate grade is induced by repeated bleedings repair the hemoglobin loss much more rapidly when given subcutaneous injections of hemoglobin than when this is not the case. But the effect of the injections is not manifest for several weeks, during which many pale corpuscles are put out by the marrow; whence it follows that the introduced pigment does not find its way in quantity direct to the new-formed cells but must follow a more or less roundabout metabolic route, perhaps the same one as that of ordinary iron compounds destined for the blood. The rapid replacement of the circulating hemoglobin in the injected animals occurs chiefly through an increased production of corpuscular substance having the same color index as that found in uninjected, anemic controls.

EFFECT OF BILE ON THE CLOTTING TIME OF BLOOD.

1. Within certain limits the clotting time of blood, of blood plasma, and of solutions of fibrin to which bile salts have been added, is proportional to the quantity of bile present. 2.