Publications by authors named "Hae-Jun Lee"
Background: Cerebral organoids (COs) are the most advanced in vitro models that resemble the human brain. The use of COs as a model for Alzheimer's disease (AD), as well as other brain diseases, has recently gained attention. This study aimed to develop a human AD CO model using normal human pluripotent stem cells (hPSCs) that recapitulates the pathological phenotypes of AD and to determine the usefulness of this model for drug screening.
View Article and Find Full Text PDFis a plant widespread in East Asia, used in folk medicine to treat various disorders, such as pneumonia, colitis, stomatitis, and carbuncle. Whether the essential oil from (ECB) and its active constituents have anti-proliferative activities in lung cancer is unknown. Therefore, we investigated the cytotoxic effects of ECB in A549 and NCI-H358 human lung cancer cells.
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- * The study found that SiONP exposure led to increased inflammatory cells and cytokines in the lung fluid, indicating a heightened inflammatory response and airway damage.
- * The research concluded that SiONPs are likely harmful to the respiratory system, especially for individuals with pre-existing respiratory conditions, due to their ability to worsen inflammation when combined with LPS.
Article Synopsis
- The study explored the anti-inflammatory effects of ethanol extracts from Potentilla supina (EPS) in both macrophage cells and mice exposed to lipopolysaccharide (LPS), a substance that triggers inflammation.
- EPS effectively reduced the production of several inflammatory markers, such as nitric oxide and various cytokines, by inhibiting key signaling pathways related to inflammation.
- In an animal model of endotoxemia, EPS not only decreased inflammatory gene expression but also improved survival rates, indicating its potential as a therapeutic agent for inflammation-related conditions.
Heat shock protein 27 (HSP27, HSPB1) is an anti-apoptotic protein characterized for its tumorigenic and metastatic properties, and now referenced as a major therapeutic target in many types of cancer. The biochemical properties of HSP27 rely on a structural oligomeric and dynamic organization that is important for its chaperone activity. Down-regulation by small interfering RNA or inhibition with a dominant-negative mutant efficiently counteracts the anti-apoptotic and protective properties of HSP27.
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- A study explored the effects of chikusetsusaponin IVa (CS) and its methyl ester (CS-ME) on inflammation in RAW264.7 macrophages exposed to lipopolysaccharide (LPS).
- CS-ME was found to be more effective than CS in inhibiting the production of nitric oxide (NO) and prostaglandin E2 (PGE2), as well as key inflammatory cytokines like TNF-α, IL-6, and IL-1β.
- The anti-inflammatory effects of CS-ME are likely due to its ability to decrease the expression of enzymes and proteins involved in inflammation (iNOS, COX-2) by downregulating transcription factors NF
α-Solanine, a trisaccharide glycoalkaloid, has been reported to possess anti-cancer effects. In this study, we investigated the anti-inflammatory effects of α-solanine isolated from "Jayoung" a dark purple-fleshed potato by examining its in vitro inhibitory effects on inducible nitric-oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and pro-inflammatory cytokines in LPS-induced RAW 264.7 macrophages and its in vivo effects on LPS-induced septic shock in a mouse model.
View Article and Find Full Text PDFIn this study, we investigated the molecular mechanisms underlying the anti-inflammatory effects of α-chaconine in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages and in LPS-induced septic mice. α-Chaconine inhibited the expressions of cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α) at the transcriptional level, and attenuated the transcriptional activity of activator protein-1 (AP-1) by reducing the translocation and phosphorylation of c-Jun.
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- Endothelial cell function is essential for maintaining the balance of blood vessel formation, influencing both normal processes like wound healing and disease states such as cancer.
- Soluble heat shock protein beta-1 (HSPB1), released from endothelial cells, interacts with vascular endothelial growth factor (VEGF) to regulate this angiogenic balance, with phosphorylation of HSPB1 by VEGF reducing its secretion and activity.
- Co-culture studies and in vivo experiments demonstrate that inhibiting HSPB1 secretion promotes tumor growth, while increasing HSPB1 can suppress metastasis, highlighting its role in both angiogenesis and wound healing.